Activation and stabilization of α-chymotrypsin by cationic additives

European Journal of Biochemistry

Volumn 268, Issue 24, 2001, Pages 6491-6497

  Spreti, Nicoletta ^a   Di Profio, Pietro ^b   Marte, Luisa ^b   Bufali, Simone ^b   Brinchi, Lucia ^b   Savelli, Gianfranco ^b  

^a UNIVERSITY OF L'AQUILA   (Italy)

^b UNIVERSITY OF PERUGIA   (Italy)

Author keywords

Chymotrypsin; Cationic surfactants; Enzyme activation; Enzyme stabilization; Organic additives

Indexed keywords

4 OCTYLOXYBENZYLTRIBUTYLAMMONIUM BROMIDE; ANILIDE; BROMINE DERIVATIVE; CATIONIC SURFACTANT; CHYMOTRYPSIN A; N GLUTARYLPHENYLALANINE 4 NITROANILIDE; TETRABUTYLAMMONIUM; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION RESPONSE; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SPECTROSCOPY; SURFACE PROPERTY;

ANIMALS; CATIONS; CATTLE; CHYMOTRYPSIN; CIRCULAR DICHROISM; ENZYME STABILITY; KINETICS; SPECTROMETRY, FLUORESCENCE; SURFACE-ACTIVE AGENTS;

EID: 0035667357     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02604.x     Document Type: Article

References (29)

1. Enzyme activity and stability control by amphiphilic self-organizing systems in aqueous solutions
2. Surfactant interactions with biomembranes and proteins
3. The effect of surfactant structure on ribonuclease A - Surfactant interactions
4. Interaction between lysozyme and n-alkyl sulphates in aqueous solution
5. The binding of detergents to proteins
6. Dissociation of bovine and bacterial catalases by sodium n-dodecyl sulfate
7. The dissociation of glucose oxidase by sodium n-dodecyl sulphate
8. A microcalorimetric study of the interaction between trypsin and sodium n-dodecyl sulphate
9. The effect of aqueous surfactant solutions on alcohol dehydrogenase
10. Interaction of enzymes with surfactants in aqueous solution and in water-in-oil microemulsions
11. Stability and kinetic behaviour of some enzymes in surfactant environment
12. Protein-decorated micelle structure of sodium-dodecyl-sulfate-protein complexes as determined by neutron scattering
13. Stability and activity of potato acid phosphatase in aqueous surfactant media
14. Kinetic and structural studies on the interaction of surfactants with lipoxigenase L1 from soybeans (Glycine max)
15. Kinetic changes following modification of rat liver alcohol dehydrogenase by deoxycholate
16. The activation of Aspergillus niger catalase by sodium n-dodecyl-sulphate
17. The structure of chymotrypsin
18. Chymotrypsin - Chemical properties and catalysis
19. α-Chymotrypsin superactivity in aqueous solutions of cationic surfactants
20. α-Chymotrypsin superactivity in cethyltrialkylammonium bromide rich media
21. Kinetic and thermodynamic evidence for the existence of at least two salt-dependent forms of α-chymotrypsin at slightly acid pH
22. Physical evidence for the existence of at least two salt-dependent forms of α-chymotrypsin at slightly acid pH
23. Decarboxylation of 6-nitrobenzisoxazole-3-carboxylate ion in cationic micelles: Effect of head group size
24. Kinetic properties of a-chymotrypsin in water-in-oil microemulsions: Studies with a variety of substrates and microemulsion system
25. Micellar solubilisation of biopolymers in organic solvents. 5. Activity and conformation of α-chymotrypsin in isooctane-AOT reverse micelles
26. Structure of crystalline α-chymotrypsin. V. The atomic structure of tosyl-α-chymotrypsin at 2Å resolution
27. Structure of α-chymotrypsin refined at 1.68Å resolution