Cys359 of GrdD is the active-site thiol that catalyses the final step of acetyl phosphate formation by glycine reductase from Eubacterium acidaminophilum

European Journal of Biochemistry

Volumn 268, Issue 24, 2001, Pages 6417-6425

  Kohlstock, Ulf Martin ^a   Rücknagel, Karl Peter ^b   Reuter, Michael ^a   Schierhorn, Angelika ^b   Andreesen, Jan R ^a   Söhling, Brigitte ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b MAX PLANCK RESEARCH UNIT FOR ENZYMOLOGY OF PROTEIN FOLDING   (Germany)

Author keywords

Acetyl phosphate; Acetyl thioester; Eubacterium acidaminophilum; Glycine reductase; Thiol modification

Indexed keywords

ACETYL PHOSPHATE; CYSTEINE; GLYCINE REDUCTASE; IODOACETIC ACID; ISOENZYME; PHOSPHATE; PROTEIN; PROTEIN C; PROTEIN GRDD; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHEMICAL MODIFICATION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME DENATURATION; EUBACTERIUM; HYDROLYSIS; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS;

AMINO ACID OXIDOREDUCTASES; AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; CYSTEINE; DNA PRIMERS; ESCHERICHIA COLI; EUBACTERIUM; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; MUTAGENESIS, SITE-DIRECTED; PHOSPHATES; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SULFHYDRYL COMPOUNDS;

ACTINOBACTERIA (CLASS); ESCHERICHIA COLI; EUBACTERIUM ACIDAMINOPHILUM; UNCULTURED ACTINOMYCETE;

EID: 0035667024     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02590.x     Document Type: Article

References (30)

1. Urkinase: Structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases
2. Introduction to the physiology and biochemistry of the genus Clostridium
3. Energy conservation in anaerobic bacteria
4. Reductive cleavage of sarcosine and betaine by Eubacterium acidaminophilum via enzyme systems different from glycine reductase
5. Various functions of selenols and thiols in anaerobic gram-positive, amino cid-utilizing bacteria
6. Identification of acetyl phosphate as the product of clostridial glycine reductase: Evidence for an acyl enzyme intermediate
7. Clostridial glycine reductase: Protein C, the acetyl group acceptor, catalyzes the arsenate-dependent decomposition of acetyl phosphate
8. Isolation and characterization of a covalent selenocysteine intermediate in the glycine reductase system
9. Mechanism of action of clostridial glycine reductase: Isolation and characterization of a covalent acetyl enzyme intermediate
10. Glycine reductase protein C. Properties and characterization of its role in the reductive cleavage of Se-carboxymethyl-selenoprotein A
11. c, a component of glycine reductase from Eubacterium acidaminophilum
12. Sarcosine reductase of Tissierella creatinophila: Purification and characterization of its components
13. The substrate-specific selenoprotein B of glycine reductase from Eubacterium acidami-nophilum. Biochemical and molecular analysis
14. Selenium-dependent clostridial glycine reductase. Purification and characterization of the two membrane-associated protein components
15. Mechanistic and stereochemical studies on the glycine reductase of Clostridium sticklandii
16. A
17. 2 or formate. Description and enzymatic studies
18. Preparation of transforming deoxyribonucleic acid by phenol treatment
19. A selDABC cluster for selenocysteine incorporation in Eubacterium acidaminophilum
20. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
21. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye-binding
22. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
23. Molecular analysis of the grd operon coding for genes of the glycine reductase and of the thioredoxin system from Clostridium sticklandii
24. Carboxydothermus hydrogenoformans gen. nov., sp. nov., a CO-utilizing thermophilic anaerobic bacterium from hydrothermal environments of Kunashir Island
25. Amino acid and glucose fermentation by Treponema denticola
26. Acetate via glycine: A different form of acetogenesis
27. SOPMA: Significant improvement in protein secondary structure prediction by consensus prediction from multiple alignments
28. Lipid biosynthetic genes and a ribosomal protein gene are cotranscribed