Targeting gene-modified hematopoietic cells to the central nervous system: Use of green fluorescent protein uncovers microglial engraftment

Nature Medicine

Volumn 7, Issue 12, 2001, Pages 1356-1361

  Priller, Josef ^a   Flügel, Alexander ^b   Wehner, Tim ^a   Boentert, Matthias ^a   Haas, Carola A ^c   Prinz, Marco ^d   Fernández Klett, Francisco ^a   Prass, Konstantin ^a   Bechmann, Ingo ^a   De Boer, Bauke A ^e   Frotscher, Michael ^c   Kreutzberg, Georg W ^b   Persons, Derek A ^f   Dirnagl, Ulrich ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

^c UNIVERSITY OF FREIBURG   (Germany)

^d MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^e DEUTSCHES RHEUMA FORSCHUNGSZENTRUM   (Germany)

^f ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; BLOOD BRAIN BARRIER; CELL TRANSPLANTATION; CENTRAL NERVOUS SYSTEM; GENE TARGETING; GENE THERAPY; HEMATOPOIETIC CELL; MICROGLIA; MOUSE; NEUROPATHY; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; BLOOD-BRAIN BARRIER; BONE MARROW CELLS; BONE MARROW TRANSPLANTATION; BRAIN ISCHEMIA; CELL DIFFERENTIATION; GENE TARGETING; GENE THERAPY; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MALE; MICE; MICE, INBRED C57BL; MICROGLIA; RECOMBINANT PROTEINS; RETROVIRIDAE;

ANIMALIA;

EID: 0035658031     PISSN: 10788956     EISSN: None     Source Type: Journal    
DOI: 10.1038/nm1201-1356     Document Type: Article

References (25)

1. Microglia: A sensor for pathological events in the CNS
2. Hematopoietic cells differentiate into both microglia and macroglia in the brains of adult mice
3. From marrow to brain: Expression of neuronal phenotypes in adult mice
4. Turning blood into brain: Ceils bearing neuronal antigens generated in vivo from bone marrow
5. Retroviral.mediated transfer of the green fluorescent protein gene into murine hematopoietic cells facilitates scoring and selection of transduced progenitors in vitro and identification of genetically modified cells in vivo
6. Sustained high-level reconstitution of the hematopoietic system by preselected hematopoietic cells expressing a transduced cell-surface antigen
7. Green mice as a source of ubiquitous green cells
8. A novel gene Iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage
9. Mature microglia resemble immature antigen-presenting cells
10. Region-specific activation of microglial cells in the rat septal complex following fimbria-fornix transection
11. Mature monocytic cells enter tissues and engraft
12. Purified hematopoietic stem cells can differentiate into hepatocytes in vivo
13. Bone marrow cells regenerate infarcted myocardium
14. Cells expressing human glucocerebrosidase from a retroviral vector repopulate macrophages and central nervous system microglia after murine bone marrow transplantation
15. Perivascular microglial cells of the CNS are bone marrow-derived and present antigen in vivo
16. Bone marrow-derived elements in the central nervous system: An immunohistochemical and ultrastructural survey of rat chimeras
17. Human neural progenitor cells: Better blue than green?
18. Kinetics of central nervous system microglial and macrophage engraftment: Analysis using a transgenic bone marrow transplantation model
19. + T cells: Selective analysis in CD45-congenic normal and Toxoplasma gondii-infected bone marrow chimeras
20. Turnover of resident microglia in the normal adult mouse brain
21. Gene-marked autologous hematopoietic stem cell transplantation of autoimmune disease
22. + donor hematogenous cells in twitcher mice after bone marrow transplantation
23. Microglial activation precedes acute neurodegeneration in Sandhoff disease and is suppressed by bone marrow transplantation
24. Neuronal MCP-1 expression in response to remote nerve injury
25. Reduced brain edema and infraction in mice lacking the neuronal isoform of nitric oxide synthase after transient MCA occlusion