Construction of a Xylanase-Producing Strain of Brevibacterium lactofermentum by Stable Integration of an Engineered xysA Gene from Streptomyces halstedii JM8

Applied and Environmental Microbiology

Volumn 67, Issue 12, 2001, Pages 5425-5430

  Adham, Sirin A I ^a   Campelo, Ana B ^a   Ramos, Angelina ^a   Gil, José A ^a  

^a UNIVERSITY OF LEÓN   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ENDO-1,3-BETA-XYLANASE; XYLAN 1,4 BETA XYLOSIDASE; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYS1 PROTEIN;

ARTICLE; BACTERIUM CONJUGATION; BIOSYNTHESIS; BREVIBACTERIUM; ENZYMOLOGY; GENETIC RECOMBINATION; GENETICS; METABOLISM; PLASMID; STREPTOMYCES;

BREVIBACTERIUM; CONJUGATION, GENETIC; PLASMIDS; RECOMBINATION, GENETIC; STREPTOMYCES; SUPPORT, NON-U.S. GOV'T; XYLOSIDASES;

EID: 0035654179     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.12.5425-5430.2001     Document Type: Article

References (24)

1. 1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC 13869. Arch. Microbiol., in press.
2. 1a. Bernfeld, P. 1951. Enzymes of starch degradation and synthesis. Adv. Enzymol. 12:379-428.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantification of micrograms quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Cadenas, R. F., J. A. Gil, and J. F. Martin. 1992. Expression of Streptomyces genes encoding extracellular enzymes in Brevibacterium lactofermentum: secretion proceeds by removal of the same leader peptide as in Streptomyces lividans. Appl. Microbiol. Biotechnol. 38:362-369.
5. Fernández-González, C., J. A. Gil, L. M. Mateos, A. Schwarzer, A. Schäfer, J. Kalinowski, A. Pühler, and J. F. Martín. 1996. Construction of L-lysine-overproducing strains of Brevibacterium lactofermentum by targeted disruption of the hom and thrB genes. Appl. Microbiol. Biotechnol. 46:554-558.
6. Gosalbes, M. J., C. D. Esteban, J. L. Galán, and G. Pérez-Martínez. 2000. Integrative food-grade expression system based on the lactose regulon of Lactobacillus casei. Appl. Environ. Microbiol. 66:4822-4828.
7. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
8. Holmes, D. S., and M. Quigley. 1981. A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114:193-197.
9. Honrubia, M. P., A. Ramos, and J. A. Gil. 2001. The cell division genes ftsQ and ftsZ, but not the three downstream open reading frames YFIH, ORF5 and ORF6, are essential for growth and viability in Brevibacterium lactofermentum ATCC 13869. Mol. Gen. Genet. 265:1022-1030.
10. Honrubia, M. P., F. J. Fernández, and J. A. Gil. 1998. Identification, characterization, and chromosomal organization of the ftsZ gene of Brevibacterium lactofermentum. Mol. Gen. Genet. 259:97-104.
11. Hopwood, D. A., M. J. Bibb, K. F. Chater, T. Kieser, C. J. Bruton, H. M. Kieser, D. J. Lydiate, C. P. Smith, J. M. Ward, and H. Schrempf. 1985. Genetic manipulation of Streptomyces: a laboratory manual. John Innes Foundation, Norwich, England.
12. Janssen, G. R., and M. J. Bibb. 1993. Derivatives of pUC18 that have Bg/II sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene 124:133-134.
13. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-686.
14. Malumbres, M. 1993. Cloning and characterisation of genes involved in threonine and lysine biosynthesis in Brevibacterium lactofermentum. Ph.D. thesis. University of León, León, Spain.
15. Martín, M. C., J. C. Alonso, J. E. Suárez, and M. A. Álvarez. 2000. Generation of food-grade recombinant lactic acid bacterium strains by site-specific recombination. Appl. Environ. Microbiol. 66:2599-2604.
16. Mateos, L. M., A. Schafer, J. Kalinowski, J. F. Martín, and A. Puhler. 1996. Integration of narrow-host-range vectors from Escherichia coli into the genomes of amino acid-producing corynebacteria after intergeneric conjugation. J. Bacteriol. 178:5768-5775.
17. Mesas, J. M. 1986. Characterization of asparaginase, aspartase and aspartokinase of Corynebacterium glutamicum. Ph.D. thesis. University of León, León, Spain.
18. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Pisabarro, A., A. Correia, and J. F. Martín. 1998. Characterization of the rrnB operon of the plant pathogen Rhodococcus fascians and targeted integrations of exogenous genes at rrn loci. Appl. Environ. Microbiol. 64:1276-1282.
20. Reyes, O., A. Guyonvarch, C. Bonamy, V. Salti, F. David, and G. Leblon. 1991. 'Integron'-bearing vectors: a method suitable for stable chromosomal integration in highly restrictive corynebacteria. Gene 107:61-68.
21. Ruiz-Arribas, A., P. Sánchez, J. J. Calvete, M. Raida, J. M. Fernández-Ábalos, and R. I. Santamaría. 1997. Analysis of xysA, a gene from Streptomyces halstedii JM8 that encodes a 45-kilodalton modular xylanase, Xys1. Appl. Environ. Microbiol. 63:2983-2988.
22. Santamaría, R. I., J. A. Gil, and J. F. Martín. 1985. High-frequency transformation of Brevibacterium lactofermentum protoplasts by plasmid DNA. J. Bacteriol. 162:463-467.
23. Schäfer, A., A. Tauch, W. Jäger, J. Kalinowsski, G. Thierbach, and A. Pühler. 1994. Small mobilizable multi-purpose cloning vectors derived from Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73.
24. Smith, M. D., J. L. Flickinger, D. W. Lineberger, and B. Schmidt. 1986. Protoplast transformation in coryneform bacteria and introduction of an α-amylase gene from Bacillus amyloliquefaciens into Brevibacterium lactofermentum. Appl. Environ. Microbiol. 51:634-639.