Imaging of neuronal activity in the gut

Current Opinion in Pharmacology

Volumn 1, Issue 6, 2001, Pages 563-567

  Berghe, Pieter Vanden ^a   Bisschops, Raf ^a   Tack, Jan ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

Calcium imagina; Enteric neurons; Fluorescent dyes; Live imaging; Neuronal activation; Neuroscience; Optical imaging; Pharmacology; Techniques Methods

Indexed keywords

CALCIUM; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; FLUORESCENT DYE;

ANIMAL; CALCIUM SIGNALING; CELL CULTURE; CELL MEMBRANE POTENTIAL; CONFOCAL MICROSCOPY; CYTOLOGY; DIAGNOSTIC IMAGING; FLUORESCENCE MICROSCOPY; HUMAN; INTESTINE INNERVATION; METABOLISM; METHODOLOGY; NERVE CELL; PHYSIOLOGY; REVIEW; SYNAPTIC TRANSMISSION;

ANIMAL; CALCIUM; CALCIUM SIGNALING; CELLS, CULTURED; DIAGNOSTIC IMAGING; ENTERIC NERVOUS SYSTEM; FLUORESCENT DYES; HUMAN; INDICATORS AND REAGENTS; MEMBRANE POTENTIALS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; NEURONS; SYNAPTIC TRANSMISSION;

EID: 0035653310     PISSN: 14714892     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1471-4892(01)00097-2     Document Type: Review

References (27)

1. Costa M., Brookes S.J., Hennig G.W. Anatomy and physiology of the enteric nervous system. Gut. 47:2000;15-19.
2. Kirchgessner A.L., Tamir H., Gershon M.D. Identification and stimulation by serotonin of intrinsic sensory neurons of the submucosal plexus of the guinea pig gut: activity-induced expression of Fos immunoreactivity. J Neurosci. 12:1992;235-248.
3. Karaosmanoglu T., Irmak M.K., Seftalioglu A. Basal Fos immunoreactivity in myenteric neurons of the guinea pig. Okajimas Folia Anat Jpn. 73:1996;83-87.
4. Kirchgessner A.L., Liu M.T., Gershon M.D. In situ identification and visualization of neurons that mediate enteric and enteropancreatic reflexes. J Comp Neurol. 371:1996;270-286.
5. Pan H., Gershon M.D. Activation of intrinsic afferent pathways in submucosal ganglia of the guinea pig small intestine. J Neurosci. 20:2000;3295-3309. This paper describes a study using electrophysiology, c-fos expression and FM2-10 uptake to investigate the activation of submuscosal primary afferent neurons by 5-HT and by mucosal stimuli.
6. Grady E.F., Gamp P.D., Jones E., Baluk P., McDonald D.M., Payan D.G., Bunnett N.W. Endocytosis and recycling of neurokinin 1 receptors in enteric neurons. Neuroscience. 75:1996;1239-1254.
7. Southwell B.R., Woodman H.L., Royal S.J., Furness J.B. Movement of villi induces endocytosis of NK1 receptors in myenteric neurons from the guinea pig ileum. Cell Tissue Res. 292:1998;37-45.
8. Obaid A.L., Koyano T., Lindstrom J., Sakai T., Salzberg B.M. Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution: optical studies of nicotinic activity in an enteric plexus. J Neurosci. 19:1999;3073-3093.
9. Neunlist M., Peters S., Schemann M. Multisite optical recording of excitability in the enteric nervous system. Neurogastro Motil. 11:1999;393-402.
10. 2+ waves by enteric neural reflexes. Nature. 399:1999;62-66.
11. Stevens R.J., Publicover N.G., Smith T.K. Propagation and neural regulation of calcium waves in longitudinal and circular muscle layers of guinea pig small intestine. Gastroenterology. 118:2000;982-984.
12. 2+ concentration changes in cultured myenteric neurones. Neurogastro Motil. 12:2000;117-124. This paper describes the establishment of calcium transients as a marker for synaptic activation.
13. 2+ signaling in cultured myenteric neurons. Am J Physiol - Gastrointest Liver Physiol. 278:2000;G905-G914.
14. 2+ signaling characteristics of inhibitory and excitatory myenteric motor neurons in culture. Am J Physiol - Gastrointest Liver Physiol. 279:2000;G1121-G1127. This paper describes a study that combined retrograde labeling with DiI and calcium imaging in cultures. Retrogradely labeled cells in culture retained fluorescent dye responsiveness. Inhibitory and excitatory motor neurons displayed differential receptor expression in culture.
15. Shuttleworth C.W., Smith T.K. Action potential-dependent calcium transients in myenteric S neurons of the guinea-pig ileum. Neuroscience. 92:1999;751-762.
16. Hillsley K., Kenyon J.L., Smith T.K. Ryanodine-sensitive stores regulate the excitability of AH neurons in the myenteric plexus of guinea-pig ileum. J Neurophysiol. 84:2000;2777-2785. The authors describe a study that established the role of intracellular stores in the control of excitability of myenteric AH neurons.
17. 2+ uptake regulates afterhyperpolarizations in guinea-pig myenteric neurons [abstract A-200]. Gastroenterology. 120:2001;1053.
18. Hanani M., Lasser-Ross N. Activity-dependent changes in intracellular calcium in myenteric neurons. Am J Physiol. 273:1997;1359-1363.
19. Simeone D.M., Kimball B.C., Mulholland M.W. Acetylcholine-induced calcium signaling associated with muscarinic receptor activation in cultured myenteric neurons. J Am Coll Surg. 182:1996;473-481.
20. Kimball B.C., Mulholland M.W. Neuroligands evoke calcium signaling in cultured myenteric neurons. Surgery. 118:1995;162-169.
21. Brookes S.J., Costa M. Identification of enteric motor neurons which innervate the circular muscle of the guinea pig small intestine. Neurosci Lett. 16:1990;227-230.
22. Vogalis F., Hillsley K., Smith T. Recording ionic events from cultured, DiI-labelled myenteric neurons in the guinea-pig proximal colon. J Neurosci Methods. 96:2000;25-34.
23. Vanden Berghe P., Smith T.K. Confocal monitoring of spontaneous activity in fibers and varicosities in myenteric neuronal networks [abstract]. Gastroenterology. 120:2001;A-335.
24. Peters S., Neunlist M., Bischoff S.C., Schemann M. Optical recordings of excitability in the human enteric nervous system [abstract]. Gastroenterology. 118:2000;A185.
25. 2+ recordings from myenteric neurons in multi-layer intestinal preparations. Neurogastro Motil 2001, 13:in press.
26. Bisschops R., Vanden Berghe P., Bellon E., Raeymaekers P., Janssens J., Tack J. Optical monitoring of electrical stimulation-induced activation of the in situ myenteric plexus in the guinea pig jejunum [abstract A-717]. Gastroenterology. 120:2001;3864.
27. Takahashi A., Camacho P., Lechleiter J.D., Herman B. Measurement of intracellular calcium. Physiol Rev. 79:1999;1089-1125.