Dissociation between stem cell phenotype and NOD/SCID repopulating activity in human peripheral blood CD34+ cells after ex vivo expansion

Experimental Hematology

Volumn 29, Issue 12, 2001, Pages 1465-1473

  Danet, Gu�nahel H ^a,b   Lee, Hubert W ^b   Luongo, Jennifer L ^c   Simon, M Celeste ^a,b   Bonnet, Dominique A ^c  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c Laboratory of Molecular and Cellular Biology of Hematopoiesis   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD34 ANTIGEN; FLT3 LIGAND; GRANULOCYTE COLONY STIMULATING FACTOR; STEM CELL FACTOR; THROMBOPOIETIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD; CELL CULTURE; CELL DIVISION; CELL FUNCTION; CELL VIABILITY; CONTROLLED STUDY; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; STEM CELL;

ADP-RIBOSYL CYCLASE; ANIMALS; ANTIGENS, CD; ANTIGENS, CD34; ANTIGENS, CD38; ANTIGENS, DIFFERENTIATION; CELL DIVISION; CELLS, CULTURED; GRANULOCYTE COLONY-STIMULATING FACTOR; HEMATOPOIETIC STEM CELL MOBILIZATION; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; HUMANS; KINETICS; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED NOD; MICE, SCID; NAD+ NUCLEOSIDASE; PROTO-ONCOGENE PROTEINS C-KIT; RECEPTORS, CXCR4; REFERENCE VALUES; T-LYMPHOCYTES; THROMBOPOIETIN; TIME FACTORS; TRANSPLANTATION, HETEROLOGOUS;

EID: 0035652621     PISSN: 0301472X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0301-472X(01)00750-0     Document Type: Article

References (47)

1. Expansion in vitro of transplantable human cord blood stem cells demonstrated using a quantitative assay of their lympho-myeloid repopulating activity in nonobese diabetic-scid/scid mice
2. Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice
3. - cells in ex vivo culture during retroviral transduction without a corresponding increase in SCID repopulating cell (SRC) frequency; dissociation of SRC phenotype and function
4. + peripheral blood progenitor cells
5. Immune-deficient SCID and NOD/SCID mice models as functional assays for studying normal and malignant human hematopoiesis
6. - cells with preserved ability to engraft SCID-hu bone
7. low/- human primitive progenitors exposed to cytokines independent of their mitotic history
8. - bone marrow cells with multilineage potential at the single-cell level; key role of thrombopoietin
9. Thrombopoietin supports proliferation of human primitive hematopoietic cells in synergy with steel factor and/or interleukin-3
10. Extensive amplification and self-renewal of human primitive hematopoietic stem cells from cord blood
11. Differential growth factor requirement of primitive cord blood hematopoietic stem cell for self-renewal and amplification vs proliferation and differentiation
12. + cord blood cells after ex vivo expansion; evidence for the amplification and self-renewal of repopulating stem cells
13. + umbilical cord blood cells in a defined serum-free medium (QBSF-60) with early effect cytokines
14. + cells in nonobese diabetic/severe combined immune-deficient mice
15. Quantitative assessment of retroviral transfer of the human multidrug resistance 1 gene to human mobilized peripheral blood progenitor cells engrafted in nonobese diabetic/severe combined immunodeficient mice
16. + subset following cell division ex vivo
17. + cells into rapid division
18. Antigenic analysis of hematopoiesis
19. + marrow cells in patients with breast cancer or neuroblastoma
20. Engraftment and long-term expression of human fetal hemopoietic stem cells in sheep following transplantation in utero
21. Sustained human hematopoiesis in sheep transplanted in utero during early gestation with fractionated adult human bone marrow cells
22. Further phenotypic characterization and isolation of human hematopoietic progenitor cells using a monoclonal antibody to the c-kit receptor
23. Enhancement of murine hematopoiesis by synergistic interactions between steel factor (ligand for c-kit), interleukin-11, and other early acting factors in culture
24. c-kit is expressed by primitive human hematopoietic cells that give rise to colony-forming cells in stroma-dependent or cytokine-supplemented culture
25. Human primitive hematopoietic progenitor cells are more enriched in KITlow cells than in KIThigh cells
26. + human marrow cells that express low levels of Kit protein are enriched for long-term marrow-engrafting cells
27. +/c-kit low cells as primitive hematopoietic stem cells
28. Isolation of a candidate human hematopoietic stem-cell population
29. Expression of Thy-1 on human hematopoietic progenitor cells
30. Human fetal bone marrow early progenitors for T, B, and myeloid cells are found exclusively in the population expressing high levels of CD34
31. Optimization of retroviral gene transduction of mobilized primitive hematopoietic progenitors by using thrombopoietin, Flt3, and Kit ligands and RetroNectin culture
32. - bone marrow long-term culture-initiating cells (LTC-IC), extended LTC-IC, and murine in vivo long-term reconstituting stem cells
33. + cells expands progenitor cell numbers, preserves engraftment capacity in nonobese diabetic/severe combined immunodeficient mice, and enhances retroviral transduction efficiency
34. Efficient adenoviral vector transduction of human hematopoietic SCID-repopulating and long-term culture-initiating cells
35. Cytokine stimulation of multilineage hematopoiesis from immature human cells engrafted in SCID mice
36. Characterization of purified and ex vivo manipulated human hematopoietic progenitor and stem cells in xenograft recipients
37. Persistence of human multilineage, self-renewing lymphohematopoietic stem cells in chimeric sheep
38. lo human marrow cells after in vitro cytokine exposure
39. Multiparameter-fluorescence activated cell sorting analysis of retroviral vector gene transfer into primitive umbilical cord blood cells
40. Evaluation of ex vivo expansion potential of cord blood and bone marrow hematopoietic progenitor cells using cell tracking and limiting dilution analysis
41. + cells isolated in subcompartments of the G0/G1 phase of the cell cycle
42. The fluctuating phenotype of the lymphohematopoietic stem cell with cell cycle transit
43. Mobilization and homing of peripheral blood progenitors is related to reversible downregulation of α4 β1 integrin expression and function
44. + cells; role in transendothelial/stromal migration and engraftment of NOD/SCID mice
45. Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4
46. The human hematopoietic stem cell compartment is heterogeneous for CXCR4 expression