Improvement on lipase catalysed regioselective O-acylation of lactose: A convenient route to 2′-O-fucosyllactose

Journal of Carbohydrate Chemistry

Volumn 20, Issue 7-8, 2001, Pages 761-765

  Rencurosi, Anna ^a   Poletti, Laura ^a   Panza, Luigi ^b   Lay, Luigi ^b  

^a UNIVERSITY OF MILAN   (Italy)

^b UNIVERSITY OF EASTERN PIEDMONT   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0035648844     PISSN: 07328303     EISSN: None     Source Type: Journal    
DOI: 10.1081/CAR-100108289     Document Type: Article

References (23)

1. th anniversary.
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11. General procedure for enzymatic acylation reaction: The substrate (1 or 2a) (100 mg) suspended in the solvent (10 mL) and the activated ester (2 mL) were shaken in the presence of Candida antarctica lipase (300 mg), immobilized on macroporus acrylic resin (CHIRAZYME® L-2, c.-f. C2, lyo; purchased from Roche Diagnostics), at the temperature reported in Table 1. The enzyme was filtered off and the solvent removed at reduced pressure. The acylated product and the unreacted substrate were isolated by chromatography on silica gel with ethyl acetate/methanol 9/1.
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22. 23 (1004.80): C, 57.37; H, 6.02. Found: C, 57.42; H, 6.05.
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