An improved method for the detection and presumptive identification of Paenibacillus larvae subsp. larvae spores in honey

Journal of Apicultural Research

Volumn 40, Issue 2, 2001, Pages 59-64

  Schuch, Dulce Maria Tocchetto ^a   Madden, Robert Heaviside ^b   Sattler, Aroni ^c  

^a PECUÁRIA E ABASTECIMENTO   (Brazil)

^b Department of Agriculture and Rural Development ^*  (United Kingdom)

^c FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

American foulbrood; Brazil; Brood diseases; Culture techniques; Diagnosis; Honey; Paenibacillus larvae larvae; Spores

Indexed keywords

EID: 0035636682     PISSN: 00218839     EISSN: 20786913     Source Type: Journal    
DOI: 10.1080/00218839.2001.11101052     Document Type: Article

References (14)

1. ALIPPI, A M, (1995) Detection of Bacillus larvae spores in Argentinean honeys by using a semiselective medium. Microbiología SEM 11:343–350.
2. BAILEY, L,; LEE, D C, (1962) Bacillus larvae:its cultivation in vitro and its growth in vivo. Journal of General Microbiology 29:711–717.
3. GORDON, R E,; HAYNES, W C,; PANG, C H, (1973) The genus Bacillus. Agriculture Handbook No. 427. Agricultural Research Service, United States Department of Agriculture, USA; 283 pp.
4. HANSEN, H, (1984) The incidence of the foulbrood bacterium Bacillus larvae in honeys retailed in Denmark. Danish Research Service for Plant and Soil Science, Report No. 1725. Administrative Centre State Bee Disease Committee; Lyngby, Denmark; pp 329–336.
5. HARRISON, H,; PATEL, R,; YOUSTEN, A A, (2000) Paenibacillus associated with milky disease in Central and South American scarabs. Journal of Invertebrate Pathology 76:169–175.
6. HORNITZKY, M A Z,; CLARK, S, (1991) Culture of Bacillus larvae from bulk honey samples for the detection of American foulbrood. Journal of Apicultural Research 30:13–16.
7. MOSSEL, D A A, (1982) Fundamentos del analisis microbiológico de los alimentos. In Acribia, S A, (ed) Microbiologia de los Alimentos. Ed. Zaragoza; pp 123–172.
8. OFFICE INTERNATIONAL DES EPIZOOTIES (1996) American foulbrood. Manual of standards for diagnostic tests and vaccines:lists A and B diseases of mammals, birds and bees. Office International des Epizooties; Paris, France; pp 603–607.
9. SATTLER, A, (1993) Investigação da ocorrência de esporos de Bacillus larvae em mel no Rio Grande do Sul, Brasil, e subsçdios para a prevenção e controle da Cria Ptrida Americana. Dissertação de mestrado do Programa de Pós Graduação em Agronomia da Universidade Federal do Rio Grande do Sul. Porto Alegre, Outubro; 84 pp.
10. SATTLER, A,; LELL C,; BASSI, E A,; MASSAO, H, (1996) Diagnóstico patológico da apicultura no Estado do Paraná—CITPAR-SIDEE/FEPA (GTZ). Proceedings; XI Simpósio Estadual de Apicultura do Paraná e VIII Exposição de Equipamentos e Materiais Apícolas; Pato Branco, PR; pp 50–53.
11. SCHUCH, D M T, (2000) Barreiras Sanitárias na Apicultura—Ações do Governo Brasileiro para o controle da entrada da cria ptrida americana. Proceedings 2nd Seminário Sul Brasiliero de Apicultores; Balnerio Pinhal/RS; pp 47–52.
12. SHIMANUKI, H,; KNOX, D A, (1988) Improved method for the detection of Bacillus larvae spores in honey. American Bee Journal 128(5):353–354.
13. SHIMANUKI, H,; KNOX, D A, (1991) American Foulbrood. Diagnosis of honey bee diseases. Agriculture Handbook No. 690. Agricultural Research Service, US Department of Agriculture; pp 4–12.
14. SHIMANUKI, H,; KNOX, D A,; FURGALA, B,; CARON, D M,; WILLIAMS, J L, (1992) Diseases and pests of honey bees. In Graham, J M, (ed) The hive and the honey bee. Dadant & Sons Inc.; Hamilton, II, USA; pp 1083–1151.