Cooperative Activity of Subunits of Human Ferritin Heteropolymers in Escherichia coli

Journal of Biochemistry and Molecular Biology

Volumn 34, Issue 4, 2001, Pages 365-370

  Lee, Jung ^a   Seo, Hyang Yim ^a   Jeon, Eun Soon ^a   Park, Ok Soon ^a   Lee, Kang Min ^a   Park, Chung Ung ^a   Kim, Kyung Suk ^a  

^a Chonbuk National University   (South Korea)

Author keywords

Cooperativity; E. coli; H and L subunits; Heteropolymer; Human ferritin

Indexed keywords

EID: 0035596790     PISSN: 12258687     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Abdul-Tehrani, H., Hudson, A. J., Chang, Y. S, Timms, A. R., Hawkins, C., Williams, J. M., Harrison, P. M., Guest, J. R. and Andrews, S. C. (1999) Ferritin mutants of Escherichia coli are iron deficient and growth impaired, and fur mutants are iron deficient. J. Bacteriol. 181, 1415-1428.
2. Beveridge, T. J. (1989) Metal ions and bacteria; in Metal Ions and Bacteria, Beveridge, T. J. and Doyle, R. J. (eds.), pp. 1-29, John Wiley & Sons, New York.
3. Burnette, W. N. (1981) Western blotting: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112, 195-203.
4. Chang, S.-R., Kim, Y.-T. and Kim, K.-S. (1995) Purification and characterization of recombinant tadpole H-chain ferritin in Escherichia coli. J. Biochem. Mol. Biol. 28, 238-242.
5. Chung, Y.-J., Kim, K.-S., Jeon, E.-S., Park, K.-I. and Park, C.-U. (1998) Effect of the Vitreoscilla hemoglobin gene on the expression of the ferritin gene in Escherichia coli. J. Biol. Mol. Biol. 31, 503-507.
6. Cozzi, A., Corsi, B., Levi, S., Santambrogio, P., Albertini, A. and Arosio, P. (2000) Overexpression of wild type and mutated human ferritin H-chain in HeLa cells: in vivo role of ferritin ferroxidase activity. J. Biol. Chem. 275, 25122-25129.
7. Cozzi, A., Santambrogio, P., Levi, S. and Arosio P. (1990) Iron detoxifying activity of ferritin. FEBS Lett. 277, 119-122.
8. Harrison, P. M., Andrews, S. C., Artymiuk, P. J., Ford, G. C., Lawson, D. M., Smith, J. M. A., Treffry, A. and White, J. L. (1990) Ferritin; in Iron Transport and Storage, Ponka, P., Schulman, H. M., Woodworth, R. C. (eds.), pp. 82-101, CRC Press, Boca Raton, Florida.
9. Harrison, P. M. and Arosio, P. (1996) The ferritins: molecular properties, iron storage function and cellular regulation. Biochim. Biophys. Acta 1275, 161-203.
10. Lawson, D. M., Treffry, A., Artymiuk, P. J., Harrison, P. M., Yewdall, S. J., Lussago, A., Cesareni, G., Levi S. and Arosio P. (1989) Identification of the ferroxidase center in ferritin. FEBS Lett. 254, 207-210.
11. Levi, S., Corsi, B., Rovida, E., Cozzi, A. Santambrogio, P., Albertini, A. and Arosio, P. (1994) Construction of a ferroxidase center in human ferritin L-chain. J. Biol. Chem. 269, 30334-30339.
12. Levi, S., Salfeld, J., Franceschinelli, F., Cozzi, A., Dorner, M. H. and Arosio, P. (1989) Expression and structural and functional properties of human ferritin L-chain from Escherichia coli. Biochemistry 28, 5179-5184.
13. Levi, S., Yewdall, S. J., Harrison, P. M., Santambrogio, P., Cozzi, A., Rovida, E., Albertini, A. and Arosio, P. (1992) Evidence that H-and L-chains have cooperative roles in the iron-uptake mechanism of human ferritin. Biochem. J. 288, 591-596.
14. Mejáre, M. and Bülow, L. (2001) Metal-binding proteins and peptides in bioremediation and phytoremediation of heavy metals. Trends Biotech. 19, 67-73.
15. Min, S. Y., Park, H. Y., Kim, M. H. and Kim, J. I. (1988) High-level expression of the insecticidal crystal protein gene from Bascillus thuringiensis subsp. kurstaki HD-73 in Escherichia coli. J. Biol. Mol. Biol. (formerly Korean Biochem. J.) 21, 372-377.
16. Rose, R. E. (1988) The nucleotide sequence of pACYC184. Nucleic Acids. Res. 11, 335-355.
17. Rucker, P., Torti, F. M. and Torti, S. V. (1997) Recombinant ferritin: modulation of subunit stoichiometry in bacterial expression systems. Protein Eng. 271, 33352-33357.
18. Santambrogio, P., Levi, S., Arosio, P., Palagi, L., Vecchio, G., Lawson, D., Yewdall, S. J., Artymiuk, P. J., Harrison, P. M., Jappelli, R. and Cesareni, G. (1992) Evidence that a salt bridge in the light chain contributes to the physical stability difference between heavy and light human ferritins. J. Biol. Chem. 267, 14077-14083.
19. Santambrogio, P., Levi, S., Cozzi, A., Rovida, E., Albertini, A. and Arosio, P. (1993) Production and characterization of recombinant heteropolymers of human ferritin H and L chains. J. Biol. Chem. 268, 12744-12748.
20. Santambrogio, P., Levi, S., Cozzi, A., Corsi, B. and Arosio, P. (1996) Evidence that the specificity of iron incorporation into homopolymers of human ferritin L-and H-chains is conferred by the nucleation and ferroxidase centers. Biochem. J. 314, 139-144.