Identification by site-directed mutagenesis of amino acid residues flanking RGD motifs of snake venom disintegrins for their structure and function

Acta Biochimica et Biophysica Sinica

Volumn 33, Issue 2, 2001, Pages 153-157

  Xu, Cun Shuan ^a   Rahman, Salman ^a  

^a HENAN NORMAL UNIVERSITY   (China)

Author keywords

Disintegrin; Integrin; RGD motif; Site directed mutagenesis; Viper venom

Indexed keywords

EID: 0035590149     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Hynes R O. Integrins: Versatility, modulation and signaling in cell adhesion. Cell, 1992, 69: 11-25
2. Ruoslahti E. RGD and other recognition sequences for integrins. Ann Rev Cell Dev Biol, 1996, 12: 697-715
3. Shattil S J. Function and regulation of the integrins in hemostasis and vascular biology. Tromb Haemost, 1995, 74: 149-155
4. Loftus J C, Smith J W, Ginsberg M H. Integrin-mediated cell adhesion: The extracellular face. J Biol Chem, 1994, 269: 25235-25238
5. Calvete J J, Juergens M, Marcinkiewicz C, Romero A, Schrader M, Niewiarowski S. Disulphide-bond pattern and molecular modeling of the dimeric disintegrin EMF-10, a potent and selective integrin 5 antogonist from Eristocophis macmahoni venom. Biochem J, 2000, 345: 573-581
6. Alder M, Lazarus R A, Dennis M S, Wagner G. Solution structure of kistrin, a potent platelet aggregation inhibitor of GPIIb/IIIa anagonist. Science, 1991, 253: 7369-7372
7. Leahy D J, Hendrickson W A, Aukhil I, Erickson H P. Structure of a fibronectin type III domain from tenascin phased by MAD analysis of the selenomethionyl protein. Science, 1992, 258: 987-991
8. Tomiyama Y, Brojer E, Ruggeri Z M, Shattil S J, Smiltneck J, Gorski J, Kumar A et al. A molecular model of RGD ligands. Antibody D gene segments that direct specificity for the integrin alpha IIb beta 3. J Biol Chem, 1992, 267: 18085-18092
9. Main A L, Harvey T S, Baron M, Boyd J, Campbell I D. The three-dimensional structure of the tenth type III module of fibronectin: An insight into RGD-mediated interactions. Cell, 1992, 71: 671-678
10. Jones E Y, Harlos K, Bottomley M J, Robinson R C, Driscoll P C, Edwards R M, Clements J M et al. Crystal structure of an integrin-binding fragment of vascular cell adhesion molecule-1 at 1.8 Å resolution. Nature, 1995, 373: 539-544
11. Diamond M S, Garcia-Aguilar J, Rickford J K, Corbi A L, Springer T A. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J Cell Biol, 1993, 120: 1031-1043
12. Scarborough R M, Naughton M A, Teng W, Rose J W, Phillips D R, Nannizzi L, Arfsten A et al. Characterization of the integrin specificities of disintegrins isolated from American pit viper venoms. J Biol Chem, 1993, 268: 1058-1065
13. Lu X J, Williams J A, Deadman J J, Salmon G P, Kakkar V V, Wilkinson J M, Baruch D et al. Preferential antagonism of the interactions of the integrin alpha IIb beta 3 with immobilized glycoprotein ligands by snake-venom RGD (Arg-Gly-Asp) proteins. Evidence supporting a functional role for the amino acid residues flanking the tripeptide RGD in determining the inhibitory properties of snake-venom RGD proteins. Biochem J, 1994, 304: 929-936
14. Rahman S, Lu X, Kakkar V V, Authi K S. The integrin alpha IIb beta 3 contains distinct and interacting binding sites for snake-venom RGD (Arg-Gly-Asp) proteins. Evidence that the receptor-binding characteristics of snake-venom RGD proteins are related to the amino acid environment flanking the sequence RGD. Biochem J, 1995, 312: 223-232
15. 1 integrin complexes. Biochem J, 1998, 335: 247-257
16. Stratagene. Quikchange™ site-directed mutagenesis kit. Instruction Manual, 1999, 1-9
17. Saiki R K, Gelfand D H, Stoffel S, Scharf S J, Higuchi R, Horn G T, Mullis K B et al. Primer-directed enzymatic amplification of DMA with a thermostable DNA polymerase. Science, 1988, 239: 487-491
18. Pharmacia. GST gene fusion system. Pharmacia Biotech, 1994, 6-15
19. Pharmacia. Development technique file No. 200 Phastsystem™. Pharmacia LKB Biotech, 1997, 47-73
20. Tozer E C, Liddington R C, Sutcliffe M J, Smeeton A H, Loftus J C. Ligand binding to integrin alpha IIb beta3 is dependent on a MIDAS-like domain in the beta3 subunit. J Biol Chem, 1996, 271: 21978-21984
21. Huang T F. What have snakes taught us about integrins? Cell Mol Life Sci, 1998, 54: 527-540
22. Rahman S, Flynn G, Altken A, Patel Y, Hassain F, Lu X, Loftus J C et al. Differential recognition of snake venom proteins expressing specific Arg-Gly-Asp (RGD) sequence motifs by wilde-type and variant integrin lib: Further evidence for distinct sites of RGD ligand recognition exhibiting negative allostery. Biochem J, 2000, 345: 701-709