A new data analysis method to determine binding constants of small molecules to proteins using equilibrium analytical ultracentrifugation with absorption optics

Analytical Biochemistry

Volumn 299, Issue 1, 2001, Pages 98-107

  Arkin, Michelle ^a,b   Lear, J D ^a,b  

^a Sunesis Pharmaceuticals Inc   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CENTRIFUGATION; CENTRIFUGES; DATA HANDLING; DISSOCIATION; LIGANDS; MOLECULES; PROTEINS; STOICHIOMETRY;

ANALYTICAL ULTRACENTRIFUGATION; COMPUTATIONAL PROGRAM; DATA ANALYSIS METHODS; DISSOCIATION CONSTANT; FUNCTIONAL ASSAYS; RADIAL CONCENTRATION; SINGLE WAVELENGTH; TWO DIFFERENT WAVELENGTHS;

EQUILIBRIUM CONSTANTS;

INTERLEUKIN 2;

ABSORPTION; AQUEOUS SOLUTION; ARTICLE; BINDING AFFINITY; DATA ANALYSIS; DISSOCIATION CONSTANT; MATHEMATICAL ANALYSIS; METHODOLOGY; OPTICS; PRIORITY JOURNAL; PROTEIN BINDING; STOICHIOMETRY; ULTRACENTRIFUGATION; WAVEFORM;

EID: 0035577805     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5396     Document Type: Article

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