Identification of GlcNAcylation sites of peptides and α-crystallin using Q-TOF mass spectrometry

Journal of the American Society for Mass Spectrometry

Volumn 12, Issue 10, 2001, Pages 1106-1113

  Chalkley, Robert J ^a   Burlingame, A L ^a,b  

^a ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; MASS SPECTROMETRY; RADIOACTIVITY;

IDENTIFICATION;

PROTEINS;

MASS SPECTROMETRY;

ACYLATION; ARTICLE; CHEMICAL MODIFICATION; CIRCULAR DICHROISM; ENERGY; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDE ANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; PROTEIN MODIFICATION;

ACYLATION; AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRYSTALLINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GALACTOSE; HYDROLYSIS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EUKARYOTA;

EID: 0035567107     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1044-0305(01)00295-1     Document Type: Article

References (27)

1. Topography and Polypeptide Distribution of Terminal N-Acetylglucosamine Residues on the Surface of Intact Lymphocytes
2. O-Glycosylation of Nuclear and Cytoplasmic Proteins; Regulation analogous to phosphorylation?
3. Dynamic O-linked Glycosylation of Nuclear and Cytoskeletal Proteins
4. Glycosylation of Nuclear and Cytoplasmic Proteins. Purification and Characterization of a Uridine Diphospho-N-Acetylglucosamine; Polypeptide β-n-acetylglucosaminyltransferase
5. Dynamic Glycosylation of Nuclear and Cytosolic Proteins. Cloning and Characterization of a Unique O-GlcNAc Transferase with Multiple Tetratricopeptide Repeats
6. Purification and Characterisation of an O-GlcNAc Selective N-Acetyl-(-D-Glucosaminidase from Rat Spleen Cytosol
7. Dynamic O-Glycosylation of Nuclear and Cytosolic Proteins; Cloning and characterization of a neutral, cytosolic β-n-acetylglucosaminidase from human brain
8. O-GlcNAcylation of Key Nuclear and Cytoskeletal Proteins; Reciprocity with o-phosphorylation and putative roles in protein multimerisation
9. RNA Polymerase II is a Glycoprotein
10. c-Myc is Glycosylated at Threonine 58, a Known Phosphorylation Site and a Mutational Hot Spot in Lymphomas
11. O-linked N-AcetylGlucosamine; The yin-yang of ser/thr phosphorylation
12. Reduced O-Glycosylation of Sp1 is Associated with Increased Proteasome Susceptibility
13. Detection of O-linked N-Acetylglucosamine (O-GlcNAc) on Cytoplasmic and Nuclear Proteins
14. Selective Detection and Site-Analysis of O-GlcNAc-Modified Glycopeptides by β-Elimination and Tandem Electrospray Mass Spectrometry
15. Mass Spectrometric Determination of the Sites of O-Glycan Attachment with Low Picomolar Sensitivity
16. Characterization of O-Glycosylation Sites in MUC2 Glycopeptides by Nanoelectrospray QTOF Mass Spectrometry
17. C-Mannosylation and O-Fucosylation of the Thrombospondin Type 1 Module
18. Two Dimensional Gel Analysis of Posttranslational Modifications
19. Collisional Fragmentation of Glycopeptides by Electrospray Ionization LC/MS and LC/MS/MS; Methods for selective detection of glycopeptides in protein digests
20. Simultaneous Detection and Identification of O-GlcNAc-Modified Glycoproteins Using Liquid Chromatography-Tandem Mass Spectrometry
21. Contributions of Mass Spectrometry to Peptide and Protein Structure
22. Specific Isolation of O-linked N-Acetylglucosamine Glycopeptides from Complex Mixtures
23. Vertebrate Lens α-Crystallins are Modified by O-Linked N-Acetylglucosamine
24. Dynamic O-GlcNAcylation of the Small Heat Shock Protein αB-crystallin
25. Glycosylation of Proteins-A Major Challenge in Mass Spectrometry and Proteomics
26. Isolation and Structure Elucidation of a Novel Adipokinetic Hormone (Lom-AKH-III) from the Glandular Lobes of the Corpus Cardiacum of the Migratory Locust, Locusta Migratoria
27. The Advantages and Versatility of a High-Energy Collision-Induced Dissociation-Based Strategy for the Sequence and Structural Determination of Proteins