Identification of functional domains of the extrinsic 12 kDa protein in red algal PSII by limited proteolysis and directed mutagenesis

Plant and Cell Physiology

Volumn 42, Issue 12, 2001, Pages 1331-1337

  Okumura, Akinori ^a   Ohta, Hisataka ^a   Inoue, Yasunori ^a   Enami, Isao ^a  

^a TOKYO UNIVERSITY OF SCIENCE   (Japan)

Author keywords

Directed mutagenesis; Extrinsic 12 kDa protein; Functional domains; Limited proteolysis; Oxygen evolving PSII; Red algae

Indexed keywords

ALGAE; EUKARYOTA; RHODOPHYTA;

ALGAL PROTEIN; CALCIUM CHLORIDE; CYTOCHROME C; CYTOCHROME C-550; CYTOCHROME C550; GLUTAMYL ENDOPEPTIDASE; OXYGEN; OXYGEN EVOLVING COMPLEX, 33 KDA PROTEIN; OXYGEN-EVOLVING COMPLEX, 33 KDA PROTEIN; SERINE PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; COMPARATIVE STUDY; CYANOBACTERIUM; DRUG EFFECT; GENETICS; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; MUTATION; OXYGEN CONSUMPTION; PHOTOSYNTHESIS; PHOTOSYSTEM II; PROTEIN BINDING; RED ALGA; SEQUENCE HOMOLOGY;

ALGAE, RED; ALGAL PROTEINS; AMINO ACID SEQUENCE; CALCIUM CHLORIDE; CYANOBACTERIA; CYTOCHROME C GROUP; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTATION; OXYGEN; OXYGEN CONSUMPTION; PHOTOSYNTHETIC REACTION CENTER COMPLEX PROTEINS; PHOTOSYSTEM II PROTEIN COMPLEX; PROTEIN BINDING; SEQUENCE HOMOLOGY, AMINO ACID; SERINE ENDOPEPTIDASES;

EID: 0035544118     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/pcp/pce170     Document Type: Article

References (17)

1. Binding and functional properties of four extrinsic proteins of photosystem II from a red alga. Cyanidium caldarium, as studied by release-reconstitution experiments
2. Isolation and characterization of a Photosystem II complex from the red alga Cyanidium caldarium: Association of cytochrome c-550 and a 12 kDa protein with the complex
3. Cross-reconstitution of various extrinsic proteins and photosystem II complexes from cyanobacteria, red algae and higher plants
4. Importance of the N-terminal sequence of the extrinsic 23 kDa polypeptide in Photosystem II in ion retention in oxygen evolution
5. A new 4.8-kDa polypeptide intrinsic to the PS II reaction center, as revealed by modified SDS-PAGE with improved resolution of low-molecular-weight proteins
6. Partial degradation of the 18-kDa protein of the photosynthetic oxygen-evolving complex: A study of a binding site
7. Cloning and sequencing of the psbU gene (Accession No. AF036250) from the cyanobacterium Cyanothece sp. ATCC 51142 (PGR 98-036)
8. Partial degradation of the extrinsic 23-kDa protein of the Photosystem II complex of spinach
9. Cloning, expression of the psbU gene, and functional studies of the recombinant 12-kDa protein of photosystem II from a red alga Cyanidium caldarium
10. Determination of accurate extinction coefficients and simultaneous-equations for assaying chlorophyll-a and chlorophyll-b extracted with 4 different solvents. Verification of the concentration of chlorophyll standards by atomic-absorption spectroscopy
11. The extrinsic polypeptides of Photosystem II
12. Analysis of the psbU gene encoding the 12-kDa extrinsic protein of photosystem II and studies on its role by deletion mutagenesis in Synechocystis sp. PCC 6803
13. Binding and functional properties of two new extrinsic components, cytochrome c-550 and a 12-kDa protein, in cyanobacterial photosystem II
14. The role of cytochrome c-550 as studied through reverse genetics and mutant characterization in Synechocystis sp. PCC 6803
15. Gene sequence for the 9 kDa component of Photosystem II from the cyanobacterium Phormidium laminosum indicates similarities between cyanobacterial and other leader sequences