Characterization of human taurine transporter expressed in insect cells using a recombinant baculovirus

Protein Expression and Purification

Volumn 23, Issue 3, 2001, Pages 389-397

  Miyasaka, Takashi ^a   Kaminogawa, Shuichi ^b   Shimizu, Makoto ^b   Hisatsune, Tatsuhiro ^a   Reinach, Peter S ^c   Miyamoto, Yusei ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c STATE UNIVERSITY OF NEW YORK   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INSECTA; UNIDENTIFIED BACULOVIRUS;

CALCIUM BINDING PROTEIN; CALCIUM BINDING PROTEINS; CALNEXIN; CARRIER PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; SODIUM; TAURINE; TAURINE TRANSPORTER; TUNICAMYCIN; VIRUS DNA;

ANIMAL; ARMYWORM; ARTICLE; BACULOVIRUS; CELL LINE; DNA VECTOR; ENZYME SPECIFICITY; GENETICS; GLYCOSYLATION; HUMAN; IMMUNOBLOTTING; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PROTEIN FOLDING; RABBIT; TRANSPORT AT THE CELLULAR LEVEL;

ANIMAL; BACULOVIRIDAE; BIOLOGICAL TRANSPORT; CALCIUM-BINDING PROTEINS; CALNEXIN; CARRIER PROTEINS; CELL LINE; DNA, VIRAL; GENETIC VECTORS; GLYCOSYLATION; HUMAN; IMMUNOBLOTTING; KINETICS; MEMBRANE GLYCOPROTEINS; PROTEIN FOLDING; RABBITS; RECOMBINANT FUSION PROTEINS; SODIUM; SPODOPTERA; SUBSTRATE SPECIFICITY; TAURINE; TUNICAMYCIN;

EID: 0035543207     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1505     Document Type: Article

References (33)

1. Characterization of taurine transport in human glioma GL15 cell line: Regulation by protein kinase C
2. Regulation of taurine transport in human colon carcinoma cell lines (HT-29 and Caco-2) by protein kinase C
3. Taurine transport in skate hepatocytes. I. Uptake and efflux
4. Properties of taurine transport in a human retinal pigment epithelial cell line
5. Transport of taurine and its regulation by protein kinase C in the JAR human placental choriocarcinoma cell line
6. Cloning and expression of a cDNA encoding the transporter of taurine and β-alanine in mouse brain
7. --dependent taurine transporter that is regulated by hypertonicity
8. Cloning of the human taurine transporter and characterization of taurine uptake in thyroid cells
9. Functional characterization and chromosomal localization of a cloned taurine transporter from human placenta
10. Isolation of a cDNA encoding a taurine transporter in the human retinal pigment epithelium
11. - neurotransmitter transporters
12. Neurotransmitter transporters: New insights into structure, function and pharmacology
13. Sodium-coupled neurotransmitter transport: Structure, function and regulation
14. --dependent serotonin transporter expressed using recombinant baculovirus in insect cells
15. Baculovirus-mediated expression of neurotransmitter transporters
16. Molecular chaperones stimulate the functional expression of the cocaine-sensitive serotonin transporter
17. Promotion of tyrosinase folding in COS 7 cells by calnexin
18. Protein measurement with the Folin phenol reagent
19. -dependent high affinity taurine transporter
20. Molecular characterization and in situ localization of a mouse retinal taurine transporter
21. Substrate-specific regulation of the taurine transporter in human placental choriocarcinoma cells (JAR)
22. Cloning and expression of a high affinity taurine transporter from rat brain
23. Calnexin: A membrane-bound chaperone of the endoplasmic reticulum
24. Promotion of transferrin folding by cyclic interactions with calnexin and calreticulin
25. Endoplasmic reticulum resident protein of 90 kilodaltons associates with the T- and B-cell antigen receptors and major histocompatibility complex antigens during their assembly
26. Trends in the development of baculovirus expression vectors
27. The role of N-glycosylation in the targeting and activity of the GLYT1 glycine transporter
28. Inability to N-glycosylate the human norepinephrine transporter reduces protein stability, surface trafficking, and transport activity but not ligand recognition
29. N-linked oligosaccharides are required for cell surface expression of the norepinephrine transporter but do not influence substrate or inhibitor recognition
30. Secretion and purification of HCV E1 protein forms as glutathione-S-transferase fusion in the baculovirus insect cell system
31. A rapid one-step method to purify baculovirus-expressed human estrogen receptor to be used in the analysis of the oxytocin promoter
32. Recombinant baculovirus vectors expressing glutathione-S-transferase fusion proteins