A stable DNA tetraloop and its structural tolerance for modification

Chemistry Letters

Volumn , Issue 3, 2001, Pages 258-259

  Kawakami, Junji ^a   Yoneyama, Mari ^a   Miyoshi, Daisuke ^a   Sugimoto, Naoki ^a  

^a KONAN UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0035532122     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2001.258     Document Type: Article

References (13)

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2. F. H. Allain, C. C. Gubser, P. W. Howe, K. Nagai, D. Neuhaus, and G. Varani, Nature, 380, 646 (1996).
3. O. C. Uhlenbeck, Nature, 346, 613 (1990).
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5. H. A. Heus and A. Pardi, Science, 253, 191 (1991).
6. V. P. Antao, S. Y. Lai, and I. Tinoco, Jr., Nucleic Acids Res., 21, 5901 (1991).
7. J. K. James and I. Tinoco, Jr., Nucleic Acids Res., 21, 3287 (1993).
8. E. G. Richerds, "Handbook of Biochemistry and Molecular Biology," 3rd ed., ed. by G. D. Fasman, CRC Press, Cleveland (1975), Nucleic Acids Vol. 1, pp. 596-603.
9. All the DNAs were synthesized by solid phase phosphoramidite method and purified by reverse phase HPLC. Concentration of DNAs was determined from the absorbance at 260 nm at high temperature with extinction coefficients calculated as described in ref 8. It was assumed that extinction coefficient of a hairpin containing abasic site was the sum of two regions separated by the abasic site.
10. V. P. Antao and I. Tinoco, Jr., Nucleic Acids Res. 1992, 20, 819-824.
11. M. Petersheim and D. H. Turner, Biochemistry, 22, 256 (1983).
12. 2EDTA (pH 7.0). All thermodynamic parameters for each hairpin formation were determined by the curve fitting procedure on the collected melting data as described in ref 11. Oligomer concentration for UV analysis was 7 μM.
13. -1 with response time of 4.0 s and bandwidth of 2.0 nm for at least 3 times scan.