Using 2′, 7′-dichlorodihydrofluorescein-diacetate to assess polysaccharides as immunomodulating agents

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 19, Issue 2, 2001, Pages 125-131

  Bland, E J ^a   Keshavarz, T ^a   Bucke, C ^a  

^a UNIVERSITY OF WESTMINSTER   (United Kingdom)

Author keywords

2 , 7 Dichlorodihydrofluorescein diacetate; Immunomodulation; Neutrophils; Polymorphonuclear leukocytes; Polysaccharides; Reactive oxygen species

Indexed keywords

DICHLORODIHYDROFLUORESCEIN DIACETATE; FLUORESCEIN DERIVATIVE; IMMUNOMODULATING AGENT; POLYSACCHARIDE; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG; 2',7' DICHLORODIHYDROFLUORESCEIN DIACETATE; 2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE; DYE; IMMUNOLOGICAL ADJUVANT;

ANALYTIC METHOD; ARTICLE; HUMAN; IMMUNOMODULATION; NEUTROPHIL; CHEMICAL MODEL; CHEMISTRY; METABOLISM; SACCHAROMYCES CEREVISIAE; SALMONELLA TYPHIMURIUM; SPECTROFLUOROMETRY; TIME;

ADJUVANTS, IMMUNOLOGIC; DYES; FLUORESCEINS; HUMAN; MODELS, CHEMICAL; NEUTROPHILS; POLYSACCHARIDES; REACTIVE OXYGEN SPECIES; SACCHAROMYCES CEREVISIAE; SALMONELLA TYPHIMURIUM; SPECTROMETRY, FLUORESCENCE; TIME FACTORS;

EID: 0035490510     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:19:2:125     Document Type: Article

References (15)

1. Lipopolysaccharide isolated from Porphyromonas gingivalis grown in hemin-limited chemostat conditions has a reduced capacity for neutrophil priming
2. Soluble β-glucan polysaccharide binding to the lectin site of neutrophil or natural killer cell complement receptor 3 (CD11b/CD18) generates a primed state of the receptor capable of mediating cytotoxicity of iC3b-opsonized target cells
3. Influence of polysaccharides on neutrophil function: Specific antagonists suggest a model for cooperative saccharide-associated inhibition of immune complex-triggered superoxide production
4. Leukocyte oxidase: Defective activity in chronic granulomatous disease
5. A new quantitative nitroblue tetrazolium reduction assay based on kinetic colorimetry
6. 2R123)
7. Induction of tumoricidal activity of polymorphonuclear leukocytes by a linear β-1,3-D-glucan and other immunomodulators in murine cells
8. Functional evaluation of human neutrophils. Is the bactericidal activity correlated with nitroblue tetrazolium reduction?
9. Further characterization of the neutrophil oxidative burst by flow cytometry
10. Fluorescent microplate assay for respiratory burst of PMNs challenged in vitro with orthopaedic metals
11. Flow cytometric analysis of the granulocyte respiratory burst: A comparison study of fluorescent probes
12. Flow cytometric analysis of nitric oxide production in neutrophils using dichlorofluorescein diacetate in the presence of a calmodulin inhibitor
13. A microplate assay for the detection of oxidative products using 2′,7′-dichlorofluorescin-diacetate