Congo red agar plate method: Improved accuracy and new extended application to Staphylococcus aureus

New Microbiologica

Volumn 24, Issue 4, 2001, Pages 355-363

  Arciola, C R ^a,b   Campoccia, D ^a   Borrelli, A M ^a   Donati, M E ^a   Montanaro, L ^a,b  

^a RIZZOLI ORTHOPAEDIC INSTITUTE   (Italy)

^b UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Biofilm; CRA test; Ico locus; Polymerase chain reaction (PCR); Slime; Staphylococcus aureus; Staphylococcus epidermidis

Indexed keywords

AGAR; COLORING AGENT; CONGO RED; N ACETYLGLUCOSAMINYLTRANSFERASE; N ACETYLLACTOSAMINIDE BETA 1,6 N ACETYLGLUCOSAMINYLTRANSFERASE; N-ACETYLLACTOSAMINIDE BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE;

ARTICLE; GENETIC SCREENING; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; PHENOTYPE; POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS;

AGAR; COLORING AGENTS; CONGO RED; GENETIC SCREENING; MICROBIOLOGICAL TECHNIQUES; N-ACETYLGLUCOSAMINYLTRANSFERASES; PHENOTYPE; POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 0035487626     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. AMMENDOLIA, M.G., DI ROSA, R., MONTANARO, L., ARCIOLA, C.R., and BALDASSARRI, L. (1999). Slime production and expression of the slime-associated antigen by staphylococcal clinical isolates. Journal of Clinical Microbiology, 37, 32355-3238.
2. ARCIOLA, C.R., COLLAMATI, S., DONATI, E., and MONTANARO, L. (2001). A rapid PCR-method for the detection of slime-producing strains of Staphylococcus epidermidis and Staphylococcus aureus in periprosthesis infections. Diagnostic Molecular Pathology, 10, 130-137.
3. ARCIOLA, C.R., BALDASSARRI, L., and MONTANARO, L. (2001). Presence of icaA and icaD genes and slime production in a collection of staphylococcal strains from catheter-associated infections. Journal of Clinical Microbiology, 39, 2151-2156.
4. CRISTHENSEN, G.D., SIMPSON, W.A., BISNO, A.L., and BEACHEY, E.H. (1982). Adherence of slime-producing Staphyloccus epidermidis to smooth surfaces. Infection and Immunity 37, 318-326.
5. CHRISTENSEN, G.D., SIMPSON, W.A., ANGLEN, J.O., and GAINOR, B.J. (2000). Methods for evaluating attached bacteria and biofilms. In: An YH and Friedmann RJ, editors. Handbook of bacterial adhesion. Principles, Methods, and Applications. Totowa, New Jersey: Humana Press Inc., pp. 213-233.
6. CRAMTON, S.E., GERKE, C., SCHNELL, N.F., NICHOLS, W.W., and GOTZ, F. (1999). The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infection and Immunity, 67, 5427-33.
7. FREEMAN, D.J., FALKINER, F.R., and KEANE, C.T. (1989). New method for detecting slime production by coagulase-negative staphylococci. Journal of Clinical Pathology, 42, 872-874.
8. GERKE, C., KRAFT, A., SUSSMUTH, R., SCHWEITZER, O., and GOTZ, F. (1998). Characterisation of the N-acetylglucosaminyltransferase activity involved in the biosynthesis of the Staphylococcus epidermidis polysaccharide intercellular adhesin. The Journal of Biological Chemestry, 273, 18586-18593.
9. MACK, D., HAEDER, M., SIEMSSEN, N., and LAUFS, R. (1996). Association of biofilm production of coagulase-negative staphylococci with expression of a specific polysaccharide intercellular adhesin. Journal of Infections Diseases, 174 (4), 881-4.
10. MACK, K.D., BARTSCHT, S., DOBINSKY, M.A., HORSTKOTTE, H., KIEL, K.-M., KNOBLOCH, A., and SCHAFER, P. (2000). Staphylococcal factors involved in adhesion and biofilm formation on biomaterials. In: An YH and Friedmann RJ, editors. Handbook of bacterial adhesion. Principles, Methods, and Applications. Totowa, New Jersey: Humana Press Inc. pp. 307-330.
11. MCKENNEY, D., HÜBNER, J., MULLER, E., WANG, Y., GOLDMANN, D.A., and PIER, G.B. (1998). The ica locus of Staphylococcus epidermidis encodes production of the capsular polysaccharide/adhesin. Infection and Immunity, 66(10), 4711-20.
12. MONTANARO, L., ARCIOLA, C.R., BALDASSARRI, L., and BORSETTI, E. (1999). Presence and expression of collagen adhesin gene (cna) and slime production in Staphylococcus aureus strains from orthopaedic prosthesis infections. Biomaterials, 20(20), 1945-9.
13. MULLER, E., TAKEDA, S., SHIRO, H., GOLDMANN, D., and PIER, G.B. (1993). Occurrence of capsular polysaccharide/adhesine among clinical isolates of coagulase-negative staphylococci. Journal of Infections Diseases, 168(5), 1211-18.
14. ZIEBUHR, W., HEILMANN, C., GÖTZ, F., MEYER, P., WILMS, K., STRAUBE, E., and HACKER, J. (1997). Detection of the intercellular adhesion gene cluster (ica) and phase variation in Staphylococcus epidermidis blood culture strains and mucosal isolates. Infection and Immunity, 65(3), 890-896.