Validation of flow cytometric competitive binding protocols and characterization of fluorescently labeled ligands

Cytometry

Volumn 45, Issue 2, 2001, Pages 102-114

  Waller, Anna ^a,c   Pipkorn, David ^b,c   Sutton, Karyn L ^c   Linderman, Jennifer J ^b,c   Omann, Geneva M ^a,b,c  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^c VA ANN ARBOR HEALTHCARE SYSTEM   (United States)

Author keywords

Binding kinetics; Flow cytometry; Fluorescence calibration; G protein coupled receptor; N formyl peptide receptor; Neutrophils

Indexed keywords

FLUORESCEIN; FORMYLNORLEUCYLLEUCYLPHENYLALANYLNORLEUCYLTYROSYLLYSINE FLUORESCEIN; FORMYLNORLEUCYLLEUCYLPHENYLALANYLNORLEUCYLTYROSYLLYSINE TETRAMETHYLRHODAMINE; FORMYLPEPTIDE; GUANINE NUCLEOTIDE BINDING PROTEIN; LIGAND; RHODAMINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; COMPETITIVE INHIBITION; DESENSITIZATION; DISSOCIATION CONSTANT; DRUG RECEPTOR BINDING; FLOW CYTOMETRY; LIGAND BINDING; MOLECULAR INTERACTION; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; RECEPTOR AFFINITY; SPECTROFLUOROMETRY; VALIDATION PROCESS;

BINDING, COMPETITIVE; CALIBRATION; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; LIGANDS; NEUTROPHILS; OLIGOPEPTIDES; PROTEIN BINDING; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, FLUORESCENCE;

EID: 0035477153     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20011001)45:2<102::AID-CYTO1152>3.0.CO;2-Z     Document Type: Article

References (24)

1. Kinetic modeling approaches to understanding ligand efficacy
2. Ligand/receptor internalization: A spectroscopic analysis and a comparison of ligand binding, cellular response, and internalization by human neutrophils
3. Real-time analysis of the assembly of ligand, receptor, and G-protein by quantitative fluorescence flow cytometry
4. Ligand binding, endocytosis, and processing
5. Competitive binding kinetics in ligand-receptor-competitor systems: Rate parameters for unlabeled ligands for the formyl peptide receptor
6. Interconverting receptor states at 4 degrees C for the neutrophil N-formyl peptide receptor
7. Strategies for positioning fluorescent probes and crosslinkers on formyl peptide ligands
8. Formyl peptide chemoattractants: A model of the receptor on rabbit neutrophils
9. A series of six ligands for the human formyl peptide receptor: Tetrapeptides with high chemotactic potency and efficacy
10. Real-time spectroscopic analysis of ligand-receptor dynamics
11. Three states for the formyl peptide receptor on intact cells
12. Fluoresceinated chemotactic peptide and high-affinity antifluorescein antibody as a probe of the temporal characteristics of neutrophil stimulation
13. Receptor mediated internalization of fluorescent chemotactic peptide by human neutrophils
14. Use of resonance interaction in the study of the chain folding of insulin in solution
15. Neutrophil degranulation detected by right angle light scattering: Spectroscopic methods suitable for simultaneous analyses of degranulation or shape change, elastase release, and cell aggregation
16. A high yield, high-purity elutriation method for preparing human granulocytes demonstrating enhanced experimental lifetimes
17. Interactions of un-ionized fluorescein. I. Color discharge by solvents and colloids
18. Fluorescence analysis of the size of a binding pocket of a peptide receptor at natural abundance
19. Analysis of ligand-receptor interactions with the fluorescence activated cell sorter
20. Receptor up-regulation, internalization, and interconverting receptor states. Critical components of a quantitative description of N-formyl peptide-receptor dynamics in the neutrophil
21. The dynamics of ligand-receptor interactions. Real-time analyses of association, dissociation, and internalization of an N-formyl peptide and its receptors on the human neutrophil
22. Evidence for protonation in the human neutrophil formyl peptide receptor binding pocket