Primary Culture of Porcine Mammary Epithelial Cells as a Model System for Evaluation of Milk Protein Expression

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 9, 2001, Pages 2098-2101

  Kumura, Haruto ^c   Tanaka, Atsushi ^c   Abo, Youichi ^c   Yui, Saori ^a   Shimazaki, Kei Ichi ^c   Kobayashi, Eiji ^b   Sayama, Kazuhiko ^a  

^a SHIZUOKA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

^c HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Epithelial cell; Milk protein expression; Porcine mammary; Primary culture

Indexed keywords

SUIDAE;

HYDROCORTISONE; INSULIN; MILK PROTEIN; PROLACTIN;

ANIMAL; ARTICLE; BAGG ALBINO MOUSE; CELL CULTURE; CELL DIFFERENTIATION; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; EPITHELIUM CELL; FEMALE; GENETICS; METABOLISM; METHODOLOGY; MOUSE; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SWINE; UDDER;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELLS, CULTURED; CULTURE MEDIA, SERUM-FREE; EPITHELIAL CELLS; FEMALE; HYDROCORTISONE; INSULIN; MAMMARY GLANDS, ANIMAL; MICE; MICE, INBRED BALB C; MILK PROTEINS; PROLACTIN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SWINE;

EID: 0035460686     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.2098     Document Type: Article

References (24)

1. Wall, R. J., Kerr, D. E., and Bondioli, K. R., Transgenic dairy cattele: Genetic engineering on a large scale. J. Dairy Sci., 80, 2213-2224 (1997).
2. Simpson, K. J., Bird, P., Shaw, D., and Nicholas, K., Molecular characterisation and hormone-dependent expression of the porcine whey acidic protein gene. J. Mol. Endocrinol., 20, 27-35 (1998).
3. Mercier, J. C. and Vilotte, J. L., Structure and function of milk protein genes. J. Dairy Sci., 76, 3079-3098 (1993).
4. Shamay, A., Solinas, S., Pursel, V. G., McKnight, R. A., Alexander, L., Beattie, C., Hennighausen, L., and Wall, R. J., Production of the mouse whey acidic protein in transgenic pigs during lactation. J. Anim. Sci., 69, 4552-4562 (1991).
5. Wall, R. J., Pursel, V. G., Shamay, A., McKnight, R. A., Pittius, C. W., and Hennighausen, L., High-level synthesis of a heterologous milk protein in the mammary glands of transgenic swine. Proc. Natl. Acad. Sci. USA, 88, 1696-1700 (1991).
6. Velander, W. H., Johnson, J. L., Page, R. L., Russell, C. G., Subramanian, A., Wilkins, T. D., Gwazdauskas, F. C., Pittius, C., and Drohan, W. N., High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C. Proc. Natl. Acad. Sci. USA, 89, 12003-12007 (1992).
7. Jerry, D. J., Stover, R. K., and Kensinger, R. S., Quantitation of prolactin-dependent responses in porcine mammary explants. J. Anim. Sci., 67, 1013-1019 (1989).
8. Houdebine, L. M., Production of pharmaceutical proteins from transgenic animals. J. Biotechnol., 34, 269-287 (1994).
9. Maga, E. A. and Murray, J. D., Mammary gland expression of transgenes and the potential for altering the properties of milk. Bio/Technol., 13, 1452-1457 (1995).
10. Richards, J., Larson, L., Yang, J., Guzman, R., Tomooka, Y., Osborn, R., Imagawa, W., and S. Nandi, Method for culturing mammary epithelial cells in a rat tail collagen gel matrix. J. Tissue Culture Methods, 8, 31-36 (1983).
11. Imagawa, W., Tomooka, Y., Yang, J., Guzman, R., Richard, J., and Nandi, S., Isolation and serum-free cultivation of mammary epithelial cells within a collagen gel matrix. In "Cell Culture Methods for Molecular Cell Biology", Vol. 2, eds. Barnes, D., Sirbasku, D., and Sato, G., Alan R. Liss, Inc., New York, pp. 127-141 (1984).
12. Chomczynski, P. and Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159 (1987).
13. Kumura, H., Sone, T., Shimazaki, K., and Kobayashi, E., Sequence analysis of porcine polymeric immunoglobulin receptor from mammary epithelial cells present in colostrum. J. Dairy Res., 67, 631-636 (2000).
14. Erhardt, V. G., Isolierung und Charakterisierung von Caseinfraktionen sowie deren genetische Varianten in Schweinemilch. Milchwissenschaft, 44, 17-20 (1989).
15. Chu, R. M., Wang, S. R., Weng, C. N., and Pursel, V. G., Isolation and characterization of porcine milk lactoferrin. Am. J. Vet. Res., 54, 1154-1159 (1993).
16. Nishikawa, Y., Xuan, X., and Otsuka, H., Biosynthesis and interaction of glycoproteins E and I of canine herpesvirus. Virus Res., 61, 11-18 (1999).
17. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.), 227, 680-685 (1970).
18. Emerman, J. T., Enami, J., Pitelka, D. R., and Nandi, S., Hormonal effects on intracellular and secreted casein in cultures of mouse mammary epithelial cells on floating collagen membranes. Proc. Natl. Acad. Sci. USA, 74, 4466-4470 (1977).
19. Talhouk, R. S., Neiswander, R. L., and Schanbacher, F. L., Morphological and functional differentiation of cryopreserved lactating bovine mammary cells cultured on floating collagen gels. Tissue & Cell, 25, 799-816 (1993).
20. Levieux, D. and Ollier, A., Bovine immunoglobulin G, beta-lactoglobulin, alpha-lactalbumin and serum albumin in colostrum and milk during the early post partum period. J. Dairy Res., 66, 421-430 (1999).
21. Puissant, C., Attal, J., and Houdebine, L. M., The hormonal control of ovine beta-lactoglobulin gene in cultured ewe mammary explants. Reprod. Nutr. Dev., 30, 245-251 (1990).
22. Nuijens, J. H., Van Berkel, P. H. C., and Schanbacher, F. L., Structure and biological actions of lactoferrin. J. Mammary GlandBiol. Neoplasia, 1, 285-295 (1996).
23. Molenaar, A. J., Kuys, Y. M., Davis, S. R., Wilkins, R. J., Mead, P. E., and Tweedie, J. W., Elevation of lactoferrin gene expression in developing, ductal, resting, and regressing parenchymal epithelium of the ruminant mammary gland. J. Dairy Sci., 79, 1198-1208 (1996).
24. Talhouk, R. S., Neiswander, R. L., and Schanbacher, F. L., In vitro culture of cryopreserved bovine mammary cells on collagen gels: synthesis and secretion of casein and lactoferrin. Tissue & Cell, 22, 583-599 (1990).