Enzyme-assisted Preparation of D-tert.-Leucine

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 9, 2001, Pages 1977-1980

  Laumen, Kurt ^a   Ghisalba, Oreste ^a   Auer, Kurt ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Alcalase ; Bacillus licheniformis; D tert. leucine; Enzymatic hydrolysis; Protease

Indexed keywords

BACILLUS LICHENIFORMIS;

2 AMINO 3,3 DIMETHYLBUTANOIC ACID; 2-AMINO-3,3-DIMETHYLBUTANOIC ACID; DRUG DERIVATIVE; LEUCINE; PROTEINASE; SUBTILISIN; VALINE;

ARTICLE; BACILLUS; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; HYDROLYSIS; METABOLISM; METHODOLOGY; SYNTHESIS;

BACILLUS; BIOCHEMISTRY; ENDOPEPTIDASES; HYDROLYSIS; LEUCINE; SUBTILISINS; VALINE;

EID: 0035460619     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.1977     Document Type: Article

References (26)

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21. Lipase CE (Amano, 4% rel. activ.), Bioprase SP4 (Nagase, 13% rel. activ.), Prozyme 6 (Amano, 15% rel. activ.), Protease N (Amano, 60% rel. activ.), Alcalase (Novo, 100% rel. activ.).
22. Higher pH values are not recommended in order to avoid chemical hydrolysis of the substrate.
23. Laumen, K., Ph.D. Thesis, BUGH-Wuppertal, 1987
24. Novo Nordisk, Alcalase 2.5L, Type DX, PMN04666, 2.67 AU/g proteolytic activity.
25. For the isolation of (S)-2, the aqueous phase from dichloromethane extraction was brought to pH 1 by addling a concentrated HCl solution. The formed precipitate was removed by centrifugation and washed with water. (S)-2 was obtained in 90% yield with an enantiomeric purity of 95.6% (HPLC), but was not further processed.
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