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Volumn 1, Issue 3, 2001, Pages 271-281

Eutectic phases in ice facilitate nonenzymatic nucleic acid synthesis

Author keywords

Ice eutectic phases; Oligonucleotide synthesis; Oligouridylate synthesis; Prebiotic polymerization; Subzero temperatures

Indexed keywords

ICE; IMIDAZOLE DERIVATIVE; LEAD; MAGNESIUM; MANGANESE; NUCLEIC ACID; OLIGONUCLEOTIDE; PHOSPHATE; POLYMER; RNA; TIN; URIDINE; URIDINE PHOSPHATE;

EID: 0035453348     PISSN: 15311074     EISSN: None     Source Type: Journal    
DOI: 10.1089/15311070152757465     Document Type: Article
Times cited : (107)

References (53)
  • 1
    • 0028036289 scopus 로고
    • Impact melting of frozen oceans on the early Earth: Implications for the origin of life
    • Bada, J.L., Bigham, C., and Miller, S.L. (1994) Impact melting of frozen oceans on the early Earth: implications for the origin of life. Proc. Natl. Acad. Sci. USA 91, 1248-1250.
    • (1994) Proc. Natl. Acad. Sci. USA , vol.91 , pp. 1248-1250
    • Bada, J.L.1    Bigham, C.2    Miller, S.L.3
  • 2
    • 0030113688 scopus 로고    scopus 로고
    • Oligomerization of uridine phosphorimidazolides on montmorillonite: A model for the prebiotic synthesis of RNA on minerals
    • Ding, P.Z., Kawamura, K., and Ferris, J.P. (1996) Oligomerization of uridine phosphorimidazolides on montmorillonite: a model for the prebiotic synthesis of RNA on minerals. Orig. Life Evol. Biosph. 26, 151-171.
    • (1996) Orig. Life Evol. Biosph. , vol.26 , pp. 151-171
    • Ding, P.Z.1    Kawamura, K.2    Ferris, J.P.3
  • 3
    • 0033811986 scopus 로고    scopus 로고
    • Sequence- and regio-selectivity in the montmorillonite-catalyzed synthesis of RNA
    • Ertem, G. and Ferris, J.P. (2000) Sequence- and regio-selectivity in the montmorillonite-catalyzed synthesis of RNA. Orig. Life Evol. Biosph. 30, 411-422.
    • (2000) Orig. Life Evol. Biosph. , vol.30 , pp. 411-422
    • Ertem, G.1    Ferris, J.P.2
  • 5
    • 0033152027 scopus 로고    scopus 로고
    • Prebiotic synthesis on minerals: Bridging the prebiotic and RNA worlds
    • Ferris, J.P. (1999) Prebiotic synthesis on minerals: bridging the prebiotic and RNA worlds. Biol. Bull. 196, 311-314.
    • (1999) Biol. Bull. , vol.196 , pp. 311-314
    • Ferris, J.P.1
  • 6
    • 0024958872 scopus 로고
    • The adsorption of nucleotides and polynucleotides on montmorillonite clay
    • Ferris, J.P., Ertem, G., and Agarwal, V.K. (1989) The adsorption of nucleotides and polynucleotides on montmorillonite clay. Orig. Life Evol. Biosph. 19, 153-164.
    • (1989) Orig. Life Evol. Biosph. , vol.19 , pp. 153-164
    • Ferris, J.P.1    Ertem, G.2    Agarwal, V.K.3
  • 7
    • 0029876064 scopus 로고    scopus 로고
    • Synthesis of long prebiotic oligomers on mineral surfaces
    • Ferris, J.P., Hill, A.R. Jr., Liu, R., and Orgel, L.E. (1996) Synthesis of long prebiotic oligomers on mineral surfaces. Nature 381, 59-61.
    • (1996) Nature , vol.381 , pp. 59-61
    • Ferris, J.P.1    Hill Jr., A.R.2    Liu, R.3    Orgel, L.E.4
  • 8
    • 36849148382 scopus 로고
    • The RNA world
    • Gilbert, W. (1986) The RNA world. Nature 319, 618.
    • (1986) Nature , vol.319 , pp. 618
    • Gilbert, W.1
  • 9
    • 0001697505 scopus 로고
    • Chemical ligation of oligonucleotides in the presence and absence of a template
    • Gryaznov, S.M. and Letsinger, R.L. (1993) Chemical ligation of oligonucleotides in the presence and absence of a template. J. Am. Chem. Soc. 115, 3808-3809.
    • (1993) J. Am. Chem. Soc. , vol.115 , pp. 3808-3809
    • Gryaznov, S.M.1    Letsinger, R.L.2
  • 10
    • 0027746565 scopus 로고
    • The limits of template-directed synthesis with nucleoside-59-phosphoro( 2-methyl)-imidazolides
    • Hill, A.R. Jr., Orgel, L.E., and Wu, T. (1993) The limits of template-directed synthesis with nucleoside-59-phosphoro( 2-methyl)- imidazolides. Orig. Life Evol. Biosph. 23, 285-290.
    • (1993) Orig. Life Evol. Biosph. , vol.23 , pp. 285-290
    • Hill Jr., A.R.1    Orgel, L.E.2    Wu, T.3
  • 11
    • 0000328668 scopus 로고
    • Substituent control of the poly(C)-directed oligomerization of guanosine 59-phosphoroimidazolide
    • Inoue, T. and Orgel, L.E. (1981) Substituent control of the poly(C)-directed oligomerization of guanosine 59-phosphoroimidazolide. J. Am. Chem. Soc. 103, 7666-7667.
    • (1981) J. Am. Chem. Soc. , vol.103 , pp. 7666-7667
    • Inoue, T.1    Orgel, L.E.2
  • 12
    • 0020664144 scopus 로고
    • A nonenzymatic RNA polymerase model
    • Inoue, T. and Orgel, L.E. (1983) A nonenzymatic RNA polymerase model. Science 219, 859-862.
    • (1983) Science , vol.219 , pp. 859-862
    • Inoue, T.1    Orgel, L.E.2
  • 13
    • 0024278974 scopus 로고
    • Non-enzymatic template- directed synthesis on RNA random copolymers. Poly(C,A) templates
    • Joyce, G.F. and Orgel, L.E. (1988) Non-enzymatic template- directed synthesis on RNA random copolymers. Poly(C,A) templates. J. Mol. Biol. 202, 677-681.
    • (1988) J. Mol. Biol. , vol.202 , pp. 677-681
    • Joyce, G.F.1    Orgel, L.E.2
  • 14
    • 0002622819 scopus 로고    scopus 로고
    • Prospects for understanding the origin of the RNA world
    • edited by R.F. Gesteland, T.R. Cech, and J.F. Atkins, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
    • Joyce, G.F. and Orgel, L.E. (1999) Prospects for understanding the origin of the RNA world. In The RNA World, edited by R.F. Gesteland, T.R. Cech, and J.F. Atkins, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 49-77.
    • (1999) The RNA World , pp. 49-77
    • Joyce, G.F.1    Orgel, L.E.2
  • 15
    • 0028066501 scopus 로고
    • Template-directed chemistry and the origins of the RNA world
    • Kanavarioti, A. (1994) Template-directed chemistry and the origins of the RNA world. Orig. Life Evol. Biosph. 24, 479-495.
    • (1994) Orig. Life Evol. Biosph. , vol.24 , pp. 479-495
    • Kanavarioti, A.1
  • 16
    • 0024958879 scopus 로고
    • Magnesium ion catalyzed P-N bond hydrolysis in imidazolide-activated nucleotides. Relevance to template-directed synthesis of polynucleotides
    • Kanavarioti, A., Bernasconi, C.F., Doodokyan, D.L., and Alberas, D.J. (1989) Magnesium ion catalyzed P-N bond hydrolysis in imidazolide-activated nucleotides. Relevance to template-directed synthesis of polynucleotides. J. Am. Chem. Soc. 111, 7247-7257.
    • (1989) J. Am. Chem. Soc. , vol.111 , pp. 7247-7257
    • Kanavarioti, A.1    Bernasconi, C.F.2    Doodokyan, D.L.3    Alberas, D.J.4
  • 17
    • 0025675721 scopus 로고
    • Limiting concentrations of activated mononucleotides necessary for poly(C)-directed elongation of oligoguanylates
    • Kanavarioti, A., Chang, S., and Alberas, D.J. (1990) Limiting concentrations of activated mononucleotides necessary for poly(C)-directed elongation of oligoguanylates. J. Mol. Evol. 31, 462-469.
    • (1990) J. Mol. Evol. , vol.31 , pp. 462-469
    • Kanavarioti, A.1    Chang, S.2    Alberas, D.J.3
  • 18
    • 0028783562 scopus 로고
    • Large steric effect in the substitution reactionof amines with phosphoimidazolide-activated nucleosides
    • Kanavarioti, A., Stonach, M.W., Ketner, R.J., and Hurley, T.B. (1995) Large steric effect in the substitution reactionof amines with phosphoimidazolide-activated nucleosides. J. Org. Chem. 60, 632-637.
    • (1995) J. Org. Chem. , vol.60 , pp. 632-637
    • Kanavarioti, A.1    Stonach, M.W.2    Ketner, R.J.3    Hurley, T.B.4
  • 19
    • 0033212688 scopus 로고    scopus 로고
    • Relative reactivity of ribosyl 29-OH vs. 39-OH in concentrated solutions of phosphoimidazolide activated nucleotides
    • Kanavarioti, A., Lee, L.F., and Gangopadhyay, S. (1999) Relative reactivity of ribosyl 29-OH vs. 39-OH in concentrated solutions of phosphoimidazolide activated nucleotides. Orig. Life Evol. Biosph. 29, 473-487.
    • (1999) Orig. Life Evol. Biosph. , vol.29 , pp. 473-487
    • Kanavarioti, A.1    Lee, L.F.2    Gangopadhyay, S.3
  • 20
    • 0033427399 scopus 로고    scopus 로고
    • Clay catalysis of oligonucleotide formation: Kinetics of the reaction of the 59-phosphorimidazolides of nucleotides with the nonbasic heterocycles uracil and hypoxanthine
    • Kawamura, K. and Ferris, J.P. (1999) Clay catalysis of oligonucleotide formation: kinetics of the reaction of the 59-phosphorimidazolides of nucleotides with the nonbasic heterocycles uracil and hypoxanthine. Orig. Life Evol. Biosph. 29, 563-591.
    • (1999) Orig. Life Evol. Biosph. , vol.29 , pp. 563-591
    • Kawamura, K.1    Ferris, J.P.2
  • 21
    • 0034309919 scopus 로고    scopus 로고
    • Nonenzymatic template- directed synthesis of RNA from monomers
    • Kozlov, I.A. and Orgel, L.E. (2000) Nonenzymatic template- directed synthesis of RNA from monomers. Mol. Biol. 34, 781-789.
    • (2000) Mol. Biol. , vol.34 , pp. 781-789
    • Kozlov, I.A.1    Orgel, L.E.2
  • 22
    • 0017745778 scopus 로고
    • Formation of nucleoside 59-phosphoramidates under potentially prebiological conditions
    • Lohrmann, R. (1977) Formation of nucleoside 59-phosphoramidates under potentially prebiological conditions.J . Mol. Evol. 10, 137-154.
    • (1977) J . Mol. Evol. , vol.10 , pp. 137-154
    • Lohrmann, R.1
  • 23
    • 0015891843 scopus 로고
    • Prebiotic activation processes
    • Lohrmann, R. and Orgel, L.E. (1973) Prebiotic activation processes. Nature 244, 418-420.
    • (1973) Nature , vol.244 , pp. 418-420
    • Lohrmann, R.1    Orgel, L.E.2
  • 24
    • 0019333922 scopus 로고
    • Efficient metalion catalyzed template-directed oligonucleotides ynthesis
    • Lohrmann, R., Bridson, P.K., and Orgel, L.E. (1980) Efficient metalion catalyzed template-directed oligonucleotides ynthesis. Science 208, 1464-1465.
    • (1980) Science , vol.208 , pp. 1464-1465
    • Lohrmann, R.1    Bridson, P.K.2    Orgel, L.E.3
  • 25
    • 0032548015 scopus 로고    scopus 로고
    • Surface-promoted replication and exponential amplification of DNA analogues
    • Luther, A., Brandsch, R., and von Kiedrowski, G. (1998) Surface-promoted replication and exponential amplification of DNA analogues. Nature 396, 245-248.
    • (1998) Nature , vol.396 , pp. 245-248
    • Luther, A.1    Brandsch, R.2    Von Kiedrowski, G.3
  • 26
    • 0013934926 scopus 로고
    • Studies on some interactionsa nd reactions of oligonucleotides in aqueous solution
    • Naylor, R. and Gilham, P.T. (1966) Studies on some interactionsa nd reactions of oligonucleotides in aqueous solution. Biochemistry 5, 2722-2728.
    • (1966) Biochemistry , vol.5 , pp. 2722-2728
    • Naylor, R.1    Gilham, P.T.2
  • 27
  • 28
    • 0029258250 scopus 로고
    • Unnatural selection in chemical systems
    • Orgel, L.E. (1995) Unnatural selection in chemical systems. Accounts Chem. Res. 28, 109-118.
    • (1995) Accounts Chem. Res. , vol.28 , pp. 109-118
    • Orgel, L.E.1
  • 29
    • 0032103374 scopus 로고    scopus 로고
    • Polymerization on the rocks: Theoretical introduction
    • Orgel, L.E. (1998) Polymerization on the rocks: theoretical introduction. Orig. Life Evol. Biosph. 28, 227-234.
    • (1998) Orig. Life Evol. Biosph. , vol.28 , pp. 227-234
    • Orgel, L.E.1
  • 30
    • 0022330784 scopus 로고
    • RNA catalysis and the origin of life
    • Pace, N.R. and Marsh, T.L. (1985) RNA catalysis and the origin of life. Orig. Life 16, 97-116.
    • (1985) Orig. Life , vol.16 , pp. 97-116
    • Pace, N.R.1    Marsh, T.L.2
  • 31
    • 0028782887 scopus 로고
    • Effect of phosphate activating group on oligonucleotide formation on montmorillonite-the regioselective formation of 39,59-linked oligoadenylates
    • Prabahar, K.J., Cole, T.D., and Ferris, J.P. (1994) Effect of phosphate activating group on oligonucleotide formation on montmorillonite-the regioselective formation of 39,59-linked oligoadenylates. J. Am. Chem. Soc. 116,10914-10920.
    • (1994) J. Am. Chem. Soc. , vol.116 , pp. 10914-10920
    • Prabahar, K.J.1    Cole, T.D.2    Ferris, J.P.3
  • 32
    • 0033133734 scopus 로고    scopus 로고
    • Nucleoside phosphorylation: A feasible step in the prebiotic pathway to RNA
    • Reimann, R. and Zubay, G. (1999) Nucleoside phosphorylation: a feasible step in the prebiotic pathway to RNA. Orig. Life Evol. Biosph. 29, 229-247.
    • (1999) Orig. Life Evol. Biosph. , vol.29 , pp. 229-247
    • Reimann, R.1    Zubay, G.2
  • 33
    • 0031003257 scopus 로고    scopus 로고
    • Efficient oligomerization of negatively-charged b-amino acids at 220°C
    • Riu, R. and Orgel, L.E. (1997) Efficient oligomerization of negatively-charged b-amino acids at 220°C. J. Am. Chem. Soc. 119, 4791-4792.
    • (1997) J. Am. Chem. Soc. , vol.119 , pp. 4791-4792
    • Riu, R.1    Orgel, L.E.2
  • 34
    • 0028989062 scopus 로고
    • Prebiotic synthesis of 5-substituted uracils: A bridge between the RNA world and the DNA-protein world
    • Robertson, M.P. and Miller, S.L. (1995a) Prebiotic synthesis of 5-substituted uracils: a bridge between the RNA world and the DNA-protein world. Science 268, 702-705.
    • (1995) Science , vol.268 , pp. 702-705
    • Robertson, M.P.1    Miller, S.L.2
  • 35
    • 0029014714 scopus 로고
    • An efficient prebiotic synthesis of cytosine and uracil
    • Robertson, M.P. and Miller, S.L. (1995b) An efficient prebiotic synthesis of cytosine and uracil. Nature 375, 772-774.
    • (1995) Nature , vol.375 , pp. 772-774
    • Robertson, M.P.1    Miller, S.L.2
  • 36
    • 0030003509 scopus 로고    scopus 로고
    • Kinetica nd mechanistic analysis of nonenzymatic, template-directed oligoribonucleotide ligation
    • Rohatgi, R., Bartel, D.P., and Szostak, J.W. (1996) Kinetica nd mechanistic analysis of nonenzymatic, template-directed oligoribonucleotide ligation. J. Am. Chem. Soc. 118, 3332-3339.
    • (1996) J. Am. Chem. Soc. , vol.118 , pp. 3332-3339
    • Rohatgi, R.1    Bartel, D.P.2    Szostak, J.W.3
  • 37
    • 0013929987 scopus 로고
    • Conditions for purine synthesis: Did prebiotic synthesis occur at low temperatures?
    • Sanchez, R., Ferris, J.P., and Orgel, L.E. (1966) Conditions for purine synthesis: did prebiotic synthesis occur at low temperatures? Science 153, 72-73.
    • (1966) Science , vol.153 , pp. 72-73
    • Sanchez, R.1    Ferris, J.P.2    Orgel, L.E.3
  • 38
    • 0017316922 scopus 로고
    • Catalysis of internucleotide bond formation by divalent metalions
    • Sawai, H. (1976) Catalysis of internucleotide bond formation by divalent metalions. J. Am. Chem. Soc. 98, 7037-7039.
    • (1976) J. Am. Chem. Soc. , vol.98 , pp. 7037-7039
    • Sawai, H.1
  • 39
    • 0023900257 scopus 로고
    • Oligonucleotide formation catalyzed by divalent metal ions. the uniqueness of the ribosyl system
    • Sawai, H. (1988) Oligonucleotide formation catalyzed by divalent metal ions. The uniqueness of the ribosyl system. J. Mol. Evol. 27, 181-186.
    • (1988) J. Mol. Evol. , vol.27 , pp. 181-186
    • Sawai, H.1
  • 40
    • 0025280564 scopus 로고
    • Divalent metal ion-mediated phosphodiester bond formation from adenosine-59-phosphorimidazolide with glycolic acid or lactic acid in aqueous solution-a nonenzymatic model reaction for nucleotidyl transfer
    • Sawai, H. (1990) Divalent metal ion-mediated phosphodiester bond formation from adenosine-59-phosphorimidazolide with glycolic acid or lactic acid in aqueous solution-a nonenzymatic model reaction for nucleotidyl transfer. Bull. Chem. Soc. Jpn. 63, 692-696.
    • (1990) Bull. Chem. Soc. Jpn. , vol.63 , pp. 692-696
    • Sawai, H.1
  • 41
    • 0019835159 scopus 로고
    • Synthesis of 29-59-linked oligouridylates in aqueous medium using the Pb21 ion
    • Sawai, H. and Ohno, M. (1981) Synthesis of 29-59-linked oligouridylates in aqueous medium using the Pb21 ion. Chem. Pharm. Bull. 29, 2237-2245.
    • (1981) Chem. Pharm. Bull. , vol.29 , pp. 2237-2245
    • Sawai, H.1    Ohno, M.2
  • 42
    • 0034527460 scopus 로고    scopus 로고
    • Nonenzymatic templatedirected condensation of short chained oligouridylates on a poly(A) template
    • Sawai, H. and Wada, M. (2000) Nonenzymatic templatedirected condensation of short chained oligouridylates on a poly(A) template. Orig. Life Evol. Biosph. 30, 503-511.
    • (2000) Orig. Life Evol. Biosph. , vol.30 , pp. 503-511
    • Sawai, H.1    Wada, M.2
  • 43
    • 0000440002 scopus 로고
    • Uranyl ions as a highly effective catalyst for internucleotide bond formation
    • Sawai, H., Kuroda, K., and Hojo, T. (1989) Uranyl ions as a highly effective catalyst for internucleotide bond formation. Bull. Chem. Soc. Jpn. 62, 2018-2023.
    • (1989) Bull. Chem. Soc. Jpn. , vol.62 , pp. 2018-2023
    • Sawai, H.1    Kuroda, K.2    Hojo, T.3
  • 44
    • 0031292281 scopus 로고    scopus 로고
    • Helical structure formation between complementary oligonucleotides. Minimum chain length required for the TDsynthesis of oligonucleotides
    • Sawai, H., Totuka, S., and Yamamoto, H. (1997) Helical structure formation between complementary oligonucleotides. Minimum chain length required for the TDsynthesis of oligonucleotides. Orig. Life Evol. Biosph. 27, 525-533.
    • (1997) Orig. Life Evol. Biosph. , vol.27 , pp. 525-533
    • Sawai, H.1    Totuka, S.2    Yamamoto, H.3
  • 45
    • 0031582714 scopus 로고    scopus 로고
    • Speculation on the RNA precursor problem
    • Schwartz, A.W. (1997) Speculation on the RNA precursor problem. J. Theor. Biol. 187, 523-527.
    • (1997) J. Theor. Biol. , vol.187 , pp. 523-527
    • Schwartz, A.W.1
  • 46
    • 0032734004 scopus 로고    scopus 로고
    • RNA structure, metal ions and catalysis
    • Scott, W.G. (1999) RNA structure, metal ions and catalysis. Curr. Opin. Chem. Biol. 3, 705-709.
    • (1999) Curr. Opin. Chem. Biol. , vol.3 , pp. 705-709
    • Scott, W.G.1
  • 47
    • 0018341059 scopus 로고
    • The catalysis of nucleotide polymerization by compounds of divalent lead
    • Sleeper, H.L. and Orgel, L.E. (1979) The catalysis of nucleotide polymerization by compounds of divalent lead. J. Mol. Evol. 12, 357-364.
    • (1979) J. Mol. Evol. , vol.12 , pp. 357-364
    • Sleeper, H.L.1    Orgel, L.E.2
  • 48
    • 0017814505 scopus 로고
    • Formation of the imidazolides of dinucleotides under potentially prebiotic conditions
    • Sleeper, H.L., Lohrmann, R., and Orgel, L.E. (1978) Formation of the imidazolides of dinucleotides under potentially prebiotic conditions. J. Mol. Evol. 11, 87-93.
    • (1978) J. Mol. Evol. , vol.11 , pp. 87-93
    • Sleeper, H.L.1    Lohrmann, R.2    Orgel, L.E.3
  • 49
    • 0000952356 scopus 로고
    • Uracil in carbonaceous meteorites
    • Stocks, P.G. and Schwartz, A.W. (1979) Uracil in carbonaceous meteorites. Nature 282, 709-710.
    • (1979) Nature , vol.282 , pp. 709-710
    • Stocks, P.G.1    Schwartz, A.W.2
  • 50
    • 0026051902 scopus 로고
    • Template-directed synthesis of oligonucleotides under eutectic conditions
    • Stribling, R. and Miller, S.L. (1991a) Template-directed synthesis of oligonucleotides under eutectic conditions. J. Mol. Evol. 32, 289-295.
    • (1991) J. Mol. Evol. , vol.32 , pp. 289-295
    • Stribling, R.1    Miller, S.L.2
  • 51
    • 0025995311 scopus 로고
    • Attempted nonenzymatic template-directed oligomerizations on a polyadenylic acid template: Implications for the nature of the first genetic material
    • Stribling, R. and Miller, S.L. (1991b) Attempted nonenzymatic template-directed oligomerizations on a polyadenylic acid template: implications for the nature of the first genetic material. J. Mol. Evol. 32, 282-289.
    • (1991) J. Mol. Evol. , vol.32 , pp. 282-289
    • Stribling, R.1    Miller, S.L.2
  • 52
    • 85171969389 scopus 로고
    • Eine Neue Synthese von Imidazol-Derivaten
    • Weidenhagen, R. and Herrmann, R. (1935) Eine Neue Synthese von Imidazol-Derivaten. Chem. Ber. 68, 1953-1961.
    • (1935) Chem. Ber. , vol.68 , pp. 1953-1961
    • Weidenhagen, R.1    Herrmann, R.2
  • 53
    • 11944254904 scopus 로고
    • Nonenzymatic template-directed synthesis on hairpin oligonucleotides. 3. Incorporation of adenosine and uridine residues
    • Wu, T. and Orgel, L.E. (1992) Nonenzymatic template-directed synthesis on hairpin oligonucleotides. 3. Incorporation of adenosine and uridine residues. J. Am. Chem. Soc. 114, 7963-7969.
    • (1992) J. Am. Chem. Soc. , vol.114 , pp. 7963-7969
    • Wu, T.1    Orgel, L.E.2


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