High transfection efficiency of human umbilical vein endothelial cells using an optimized calcium phosphate method

Analytical Biochemistry

Volumn 296, Issue 1, 2001, Pages 143-147

  Segura, Inmaculada ^a   González, Manuel A ^a   Serrano, Antonio ^a   Abad, José Luis ^a   Bernad, Antonio ^a   Riese, Hans H ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM PHOSPHATE; ENDOTHELIAL CELLS;

HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; TRANSFECTION EFFICIENCY;

MOLECULAR BIOLOGY;

CALCIUM PHOSPHATE;

ANGIOGENESIS; CELL CULTURE; CONTROLLED STUDY; ENDOTHELIUM CELL; GENETIC TRANSFECTION; HISTOPATHOLOGY; HUMAN; HUMAN CELL; IN VITRO STUDY; METHODOLOGY; NEWBORN; NOTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; UMBILICAL VEIN; VASCULAR ENDOTHELIUM;

EID: 0035450476     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5200     Document Type: Article

References (7)

1. Culture of human endothelial cells derived from umbilical veins
2. Generation of retroviral vectors for clinical studies using transient transfection
3. High efficiency reporter gene transfection of vascular tissue in vitro and in vivo using a cationic lipid-DNA complex
4. High efficiency transformation of human endothelial cells by apoE-mediated transfection with plasmid DNA
5. Optimization of transfection of human endothelial cells
6. Efficient gene transfer into human umbilical vein endothelial cells allows functional analysis of the human tissue factor gene promoter