Use of an Intergenic Region in Pseudomonas syringae pv. Syringae B728a for Site-Directed Genomic Marking of Bacterial Strains for Field Experiments

Applied and Environmental Microbiology

Volumn 67, Issue 8, 2001, Pages 3735-3738

  Hirano, Susan S ^a   Willis, David K ^a,b   Clayton, Murray K ^a,c   Upper, Christen D ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b U S DAIRY FORAGE RESEARCH CENTER   (United States)

^c University of Wisconsin Madison   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; LEMA PROTEIN, BACTERIAL; SPACER DNA; TRANSCRIPTION FACTOR;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL COUNT; BACTERIAL GENOME; DRUG EFFECT; GENETIC MARKER; GENETIC RECOMBINATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; LEGUME; MEDICINAL PLANT; METABOLISM; MICROBIOLOGY; MUTATION; PATHOGENICITY; PLANT DISEASE; PSEUDOMONAS;

BACTERIAL PROTEINS; COLONY COUNT, MICROBIAL; DNA, INTERGENIC; DRUG RESISTANCE, MICROBIAL; FABACEAE; GENETIC MARKERS; GENOME, BACTERIAL; MUTATION; PLANT DISEASES; PLANTS, MEDICINAL; PSEUDOMONAS; RECOMBINATION, GENETIC; TRANSCRIPTION FACTORS;

EID: 0035432251     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.8.3735-3738.2001     Document Type: Article

References (17)

1. Alfano, J. R., D. W. Bauer, T. M. Milos, and A. Collmer. 1996. Analysis of the role of the Pseudomonas syringae pv. syringae HrpZ harpin in elicitation of the hypersensitive response in tobacco using functionally non-polar hrpZ deletion mutations, truncated HrpZ fragments, and hrm4 mutations. Mol. Microbiol. 19:715-728.
2. De Leij, F. A. A. M., C. E. Thomas, M. J. Bailey, J. M. Whipps, and J. M. Lynch. 1998. Effect of insertion site and metabolic load on the environmental fitness of a genetically modified Pseudomonas fluorescens isolate. Appl. Environ. Microbiol. 64:2634-2638.
3. Fellay, R., J. Frey, and H. Krisch. 1987. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52:147-154.
4. Hirano, S. S., L. S. Baker, and C. D. Upper. 1996. Raindrop momentum triggers growth of leaf-associated populations of Pseudomonas syringae on field-grown snap bean plants. Appl. Environ. Microbiol. 62:2560-2566.
5. Hirano, S. S., A. O. Charkowski, A. Collmer, D. K. Willis, and C. D. Upper. 1999. Role of the Hrp type III protein secretion system in growth of Pseudomonas syringae pv. syringae B728a on host plants in the field. Proc. Natl. Acad. Sci. USA. 96:9851-9856.
6. Hirano, S. S., M. K. Clayton, and C. D. Upper. 1994. Estimation of and temporal changes in means and variances of populations of Pseudomonas syringae on snap bean leaflets. Phytopathology 84:934-940.
7. Hirano, S. S., E. V. Nordheim, D. C. Arny, and C. D. Upper. 1982. Lognormal distribution of epiphytic bacterial populations on leaf surfaces. Appl. Environ. Microbiol. 44:695-700.
8. Hirano, S. S., E. M. Ostertag, S. A. Savage, L. S. Baker, D. K. Willis, and C. D. Upper. 1997. Contribution of the regulatory gene lemA to field fitness of Pseudomonas syringae pv. syringae. Appl. Environ. Microbiol. 63:4304-4312.
9. Hrabak, E. M., and D. K. Willis. 1992. The lemA gene required for pathogenicity of Pseudomonas syringae pv. syringae on bean is a member of a family of two-component regulators. J. Bacteriol. 174:3011-3020.
10. Hrabak, E. M., and D. K. Willis. 1993. Involvement of the lemA gene in production of syringomycin and protease by Pseudomonas syringae pv. syringae. Mol. Plant-Microbe Interact. 6:368-375.
11. King, E. O., M. K. Ward, and D. E. Raney. 1954. Two simple media for the demonstration of pyocyanin and fluorescin. J. Lab. Clin. Med. 44:301-307.
12. Kinscherf, T. G., and D. K. Willis. 1999. Swarming by Pseudomonas syringae B728a requires gacS (lemA) and gacA but not the acyl-homoserine lactone biosynthetic gene ahlI. J. Bacteriol. 181:4133-4136.
13. Kitten, T., T. G. Kinscherf, J. L. McEvoy, and D. K. Willis. 1998. A newly-identified regulator is required for virulence and toxin production in Pseudomonas syringae. Mol. Microbiol. 28:917-929.
14. Littell, R. C., G. A. Milliken, W. W. Stroup, and R. D. Wolfinger. 1996. SAS system for mixed models. SAS Institute Inc., Cary, N.C.
15. Prentki, P., and H. M. Krisch. 1984. In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29:303-313.
16. Schweizer, H. P. 1993. Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis. BioTechniques 15:831-833.
17. Willis, D. K., J. J. Holmstadt, and T. G. Kinscherf. 2001. Genetic evidence that loss of virulence associated with gacS or gacA mutations in Pseudomonas syringae B728a does not result from effects on alginate production. Appl. Environ. Microbiol. 67:1400-1403.