Mutations lowering the phosphatase activity of HPr kinase/phosphatase switch off carbon metabolism

EMBO Journal

Volumn 20, Issue 15, 2001, Pages 3928-3937

  Monedero, Vicente ^a   Poncet, Sandrine ^a   Mijakovic, Ivan ^a   Fieulaine, Sonia ^b   Dossonnet, Valérie ^a   Martin Verstraete, Isabelle ^a   Nessler, Sylvie ^b   Deutscher, Josef ^a  

^a CNRS   (France)

^b LABORATOIRE D ENZYMOLOGIE ET BIOCHIMIE STRUCTURALES   (France)

Author keywords

Bifunctional enzymes; Carbohydrate metabolism; HPr; HPr kinase:P Ser HPr phosphatase; PEP:glycose phosphotransferase system

Indexed keywords

MUTANT PROTEIN; PHOSPHOENOLPYRUVATE; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHOTRANSFERASE; PROTEIN KINASE; PROTEIN SUBUNIT; SERINE;

ALLELE; ARTICLE; BINDING SITE; CARBOHYDRATE METABOLISM; DEPHOSPHORYLATION; ENZYME ACTIVITY; GENE EXPRESSION; GENE MUTATION; GRAM POSITIVE BACTERIUM; LACTOBACILLUS CASEI; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION;

ALLELES; AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CARBOHYDRATE METABOLISM; CARBON; CONSERVED SEQUENCE; DNA-BINDING PROTEINS; GENE EXPRESSION; GENES, REPORTER; LAC OPERON; LACTOBACILLUS CASEI; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN-SERINE-THREONINE KINASES; REPRESSOR PROTEINS;

EID: 0035421189     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.15.3928     Document Type: Article

References (51)

1. ATP-dependent protein kinase-catalyzed phosphorylation of a seryl residue in HPr, a phosphate carrier protein of the phosphotransferase system in Streptococcus pyogenes
2. The bacterial phosphoenolpyruvate-dependent phosphotransferase system: P-Ser-HPr and its possible regulatory function
3. Streptococcal phosphoenolpyruvate-sugar phosphotransferase system: Amino acid sequence and site of ATP-dependent phosphorylation of HPr
4. Loss of protein kinase-catalyzed phosphorylation of HPr, a phosphocarrier protein of the phosphotransferase system, by mutation of the ptsH gene confers catabolite repression resistance to several catabolic genes of Bacillus subtilis
5. Protein kinase-dependent HPr/CcpA interaction links glycolytic activity to carbon catabolite repression in Gram-positive bacteria
6. Carbohydrate uptake and metabolism
7. Phosphorylation of HPr by the bifunctional HPr kinase/P-Ser-HPr phosphatase from Lactobacillus casei controls catabolite repression and inducer exclusion, but not inducer expulsion
8. Cloning, characterization and expression of a bifunctional fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase from potato
9. Site-directed mutagenesis with the ptsH gene of Bacillus subtilis. Isolation and characterization of heat-stable proteins altered at the ATP-dependent regulatory phosphorylation site
10. The catabolite control protein CcpA controls ammonium assimilation in Bacillus subtilis
11. X-ray structure of HPr kinase: A bacterial protein kinase with a P-loop nucleotide-binding domain
12. Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr
13. The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression
14. New protein kinase and protein phosphatase families mediate signal transduction in bacterial catabolite repression
15. Phosphorylation of either Crh or HPr mediates binding of CcpA to the Bacillus subtilis xyn cre and catabolite repression of the xyn operon
16. Cascade control of Escherichia coli glutamine synthetase. Purification and properties of PII uridylyltransferase and uridylyl-removing enzyme
17. Replacement of isoleucine-47 by threonine in the HPr protein of Streptococcus salivarius abrogates the preferential metabolism of glucose and fructose over lactose and melibiose but does not prevent the phosphorylation of HPr on serine-46
18. The crystal structure of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase reveals distinct domain homologies
19. Sequence and structure of the human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase heart isoform gene (PFKFB2)
20. Catabolite repression of α-amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the Escherichia coli lacl and galR repressors
21. Characterization of an HPr kinase mutant of Staphylococcus xylosus
22. Isocitrate dehydrogenase kinase/phosphatase: AceK alleles that express kinase but not phosphatase activity
23. The two opposing activities of adenylyl transferase reside in distinct homologous domains, with intramolecular signal transduction
24. The HPr kinase from Bacillus subtilis is a homo-oligomeric enzyme which exhibits strong positive cooperativity for nucleotide and fructose 1,6-bisphosphate binding
25. Adenylylation/deadenylylation control of the glutamine synthetase of Rhodopseudomonas capsulata
26. Binding of the catabolite repressor protein CcpA to its DNA target is regulated by phosphorylation of its corepressor HPr
27. The hprK gene of Enterococcus faecalis encodes a novel bifunctional enzyme: The HPr kinase/phosphatase
28. A protein with kinase and phosphatase activities involved in regulation of tricarboxylic acid cycle
29. Crystal structure of a trapped phosphoenzyme during a catalytic reaction
30. Induction and metabolite regulation of levanase synthesis in Bacillus subtilis
31. Phosphorylation of HPr and Crh by HprK, early steps in the catabolite repression signalling pathway for the Bacillus subtilis levanase operon
32. Locations of the regulatory sites for isocitrate dehydrogenase kinase/phosphatase
33. Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis
34. Catabolite repression mediated by CcpA protein in Bacillus subtilis: Novel modes of regulation revealed by whole-genome analyses
35. Phosphoenol-pyruvate: Carbohydrate phosphotransferase systems of bacteria
36. Catabolite regulation of the pta gene as part of carbon flow pathways in Bacillus subtilis
37. A novel protein kinase that controls carbon catabolite repression in bacteria
38. Novel phosphotransferase system genes revealed by genome analysis - The complete complement of PTS proteins encoded within the genome of Bacillus subtilis
39. Regulation of carbon catabolism in Bacillus species
40. Induction of the Bacillus subtilis ptsGHl operon by glucose is controlled by a novel antiterminator, GlcT
41. 14C fluorography in studies of glycolysis and regulation of pyruvate kinase in Streptococcus lactis
42. Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3 Å resolution
43. A vector for systematic gene inactivation in Bacillus subtilis
44. Enzyme I and HPr from Lactobacillus casei: Their role in sugar transport, carbon catabolite repression and inducer exclusion
45. Characterization of phosphorylated histidine-containing protein (HPr) of the bacterial phosphoenolpyruvate: Sugar phosphotransferase system
46. Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis
47. + symport permease of Lactobacillus brevis
48. Regulation of sugar uptake via the phosphoenolpyruvate-dependent phosphotransferase system in Bacillus subtilis and Lactococcus lactis is mediated by ATP-dependent phosphorylation of seryl residue 46 in HPr
49. Trans-acting factors affecting carbon catabolite repression of the hut operon in Bacillus subtilis
50. Phosphatase activity of histidine kinase EnvZ without kinase catalytic domain