Establishment of Specific Pathogen-Free (SPF) rat colonies using gnotobiotic techniques

Experimental Animals

Volumn 50, Issue 4, 2001, Pages 293-298

  Yanabe, Makoto ^a   Shibuya, Masaharu ^a   Gonda, Toshihiko ^a   Asai, Hidekazu ^a   Tanaka, Toshio ^a   Sudou, Katuko ^b   Narita, Teruo ^c   Itoh, Kikuji ^d  

^a Japan SLC Inc   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c DAIICHI SANKYO CO   (Japan)

^d UNIVERSITY OF TOKYO   (Japan)

Author keywords

Cecal microflora; Gnotobiote; Limited flora rats; Normalization; SPF rats

Indexed keywords

BACTEROIDACEAE; CLOSTRIDIA; CLOSTRIDIUM; CLOSTRIDIUM DIFFICILE; ESCHERICHIA COLI; LACTOBACILLUS; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; RATTUS NORVEGICUS;

ANIMAL; ANIMAL HUSBANDRY; ARTICLE; BACTEROIDACEAE; CECUM; ESCHERICHIA COLI; FECES; FEMALE; GERMFREE ANIMAL; LACTOBACILLUS; MALE; MICROBIOLOGY; POPULATION DYNAMICS; RAT; WISTAR RAT;

ANIMAL HUSBANDRY; ANIMALS; BACTEROIDACEAE; CECUM; ESCHERICHIA COLI; FECES; FEMALE; LACTOBACILLUS; MALE; POPULATION DYNAMICS; RATS; RATS, WISTAR; SPECIFIC PATHOGEN-FREE ORGANISMS;

EID: 0035409520     PISSN: 13411357     EISSN: None     Source Type: Journal    
DOI: 10.1538/expanim.50.293     Document Type: Article

References (23)

1. Deerberg, F. and Pittermann, W. 1978. The effect of germfree and SPF maintenance conditions on the lifespan and diseases of small laboratory animals. J. South African Vet. Assoc. 49: 179-182.
2. Hazenberg, M.P. and Custers-van Lieshout, L.M.C. 1976. Conversion of germ-free mice to the normal state by clostridia. Z. Versuchstierk. 18: 185-190.
3. Heine, W. 1978. Twelve years experience in establishing larger colonies of small laboratory animals under SPF conditions by means of germfree technology. J. South African Vet. Assoc. 49: 175-177.
4. Hirayama, K., Endo, K., Kawamura, S., and Mitsuoka, T. 1990. Comparison of the intestinal bacteria in specific pathogen free mice from different breeders. Exp. Anim. 39: 263-267.
5. Itoh, K. and Mitsuoka, T. 1980. Production of gnotobiotic mice with normal physiological functions. I. Selection of useful bacteria from feces of conventional mice. Z. Versuchstierk. 22: 173-178.
6. Itoh, K., Mitsuoka, T., Sudo, K., and Suzuki, K. 1983. Comparison of fecal flora of mice based upon different strains and different housing conditions. Z. Versuchstierk. 25: 135-146.
7. Itoh, K., Mitsuoka, T., Sudo, K., and Suzuki, K. 1983. Comparison of fecal lactobacilli in mice of different strains under different housing conditions. Z. Versuchstierk. 25: 193-200.
8. Itoh, K. and Mitsuoka, T. 1985. Characterization of clostridia isolated from faeces of limited flora mice and their effect on caecal size when associated with germ-free mice. Lab. Animals 19: 111-118.
9. Itoh, K., Urano, T., and Mitsuoka, T. 1986. Colonization resistance against Pseudomonas aeruginosa in gnotobiotic mice. Lab. Animals 20: 197-201.
10. Itoh, K., Lee, W.K., Kawamura, H., Mitsuoka, T., and Magaribuchi, T. 1986. Isolation of Clostridium difficile from various colonies of laboratory mice. Lab. Animals 20: 266-270.
11. Itoh, K., Lee, W.K., Kawamura, H., Mitsuoka, T., and Magaribuchi, T. 1987. Intestinal bacteria antagonistic to Clostridium difficile in mice. Lab. Animals 21: 20-25.
12. Itoh, K. and Freter, R. 1989. Control of Escherichia coli populations by a combination of indigenous clostridia and lactobacilli in gnotobiotic mice and continuous-flow cultures. Infect. Immun. 57: 559-565.
13. Koopman, J.P., Janssen, F.G.J., and van Druten, J.A.M. 1977. The relation between the intestinal microflora and intestinal parameters in mice. Z. Versuchstierk 19: 54-61.
14. Koopman, J.P., Kennis, H.M., Hectors, M.P.C., Lankhorst, A., Stadhouders, A.J., and de Boer, H. 1984. Reciprocal "normalization" of intestinal parameters by indigenous intestinal microflora of the rat and mouse. Z. Versuchstierk. 26: 289-295.
15. Koopman, J.P., Kennis, H.M., Mullink, J.W.M.A., Prins, R.A., Stadhouders, A.M., de Boer, H., Hectors, M.O.C., and van der Logt, J.T.M. 1984. Association of germ-free rats with different microfloras. Z. Versuchstierk. 26: 49-56.
16. Maejima, K., Urano, T., Itoh, K., Fujiwara, K., and Homma, J. Y. 1972. Methods for detection and isolation of Pseudomonas aeruginosa in mouse breeding colonies. Exp. Anim. 21: 13-18.
17. Morishita, Y. and Miyaki, K. 1979. Effects of age and starvation on the gastrointestinal microflora and the heat resistance of fecal bacteria in rats. Microbiol. Immunol. 23: 455-470.
18. O'rourke, J., Lee, A., and McNeill, J. 1988. Differences in the gastrointestinal microbiota of specific pathogen free mice: An often unknown variable in biomedical research. Lab. Animals 22: 297-303.
19. Trexler, P. C. 1978. A rationale for the development of gnotobiotics. Lab. Animals 12: 257-262.
20. Van der Waaiji, D., Berghuis-de Vries, J.M., and Lekkerkerk-van der Wees, J.E.C. 1971. Colonization resistance of digestive tract in conventional and antibiotic-treated mice. J. Hyg. 69: 405-411.
21. Walburg, H.E. Jr., Mynatt, E.I., Cosgrove, G.E., Tyndall, R.L., and Robie, D.M. 1965. Microbiological evaluation of an isolation facility for the production of specific-pathogen-free mice. Lab. Animal Care 15: 208-216.
22. Watanabe, T., Morotomi, M., Suegara, N., Kawai, Y., and Mutai, M. 1977. Distribution of indigenous lactobacilli in the digestive tract of conventional and gnotobiotic rats. Microbiol. Immunol. 21: 183-191.
23. Yanabe, M., Shibuya, M., Gonda, T., Asai, H., Tanaka, T., Narita, T., Sudo, K., Matsui, T., and Itoh, K. 1999. Production of ex-germfree rabbits for establishment of specific pathogen-free (SPF) colonies. Exp. Anim. 48: 79-86.