In vivo and in vitro analyses of the AmyR binding site of the Aspergillus nidulans agdA promoter; requirement of the CGG direct repeat for induction and high affinity binding of AmyR

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 7, 2001, Pages 1568-1574

  Tani, Shuji ^a   Itoh, Tomoyuki ^a   Kato, Masashi ^a   Kobayashi, Tetsuo ^a   Tsukagoshi, Norihiro ^a  

^a NAGOYA UNIVERSITY   (Japan)

Author keywords

Amylase induction; AmyR; Aspergillus nidulans; DNA binding; Transcriptional activator

Indexed keywords

ASPERGILLUS; EMERICELLA NIDULANS; ESCHERICHIA COLI;

ALPHA GLUCOSIDASE; AMYR PROTEIN, ASPERGILLUS; FUNGAL DNA; FUNGAL PROTEIN; RECOMBINANT PROTEIN; TRANSACTIVATOR PROTEIN;

ARTICLE; ASPERGILLUS NIDULANS; BINDING SITE; ENZYMOLOGY; ESCHERICHIA COLI; FUNGAL GENE; GENETICS; METABOLISM; NUCLEOTIDE REPEAT; NUCLEOTIDE SEQUENCE; PROMOTER REGION; SITE DIRECTED MUTAGENESIS;

ALPHA-GLUCOSIDASES; ASPERGILLUS NIDULANS; BASE SEQUENCE; BINDING SITES; DNA, FUNGAL; ESCHERICHIA COLI; FUNGAL PROTEINS; GENES, FUNGAL; MUTAGENESIS, SITE-DIRECTED; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; REPETITIVE SEQUENCES, NUCLEIC ACID; TRANS-ACTIVATORS;

EID: 0035403454     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.1568     Document Type: Article

References (29)

1. Boel, E., Hansen, M. T., Hjort, I., Hoegh, I., and Fiil, N. P., Two different types of intervening sequences in the glucoamylase gene from Aspergillus niger. EMBO J., 3, 1581-1585 (1984).
2. Gines, M. J., Dove, M. J., and Seligy, V. L., Aspergillus oryzae has two nearly identical Taka-amylase genes, each containing eight introns. Gene, 79, 107-111 (1989).
3. Hata, Y., Tsuchiya, K., Kitamoto, K., Gomi, K., Tamura, G., and Hara, S., Nucleotide sequence and expression of the glucoamylase-encoding gene (glaA) from Aspergillus oryzae. Gene, 108, 145-150 (1991).
4. Minetoki, T., Gomi, K., Kitamoto, K., Kumagai, C., and Tamura, G., Nucleotide sequence and expression of α-glucosidase-encoding gene (agdA) from Aspergillus oryzae. Biosci. Biotechnol. Biochem., 59, 1516-1521 (1995).
5. Tada, S., Iimura, Y., Gomi, K., Takahashi, K., Hara, S., and Yoshizawa, K., Cloning and nucleotide sequence of the genomic Taka-amylase A gene of Aspergillus oryzae. Agric. Biol. Chem., 53, 593-599 (1989).
6. Tsukagoshi, N., Furusawa, M., Nagaba, H., Kirita, N., Tuboi, A., and Udaka, S., Isolation of a cDNA encoding Aspergillus oryzae Taka-amylase A: evidence for multiple related genes. Gene, 84, 319-327 (1989).
7. Wirsel, S., Lachmund, A., Wildhardt, G., and Ruttkowski, E., Three α-amylase genes of Aspergillus oryzae exhibit identical intron organization. Mol. Microbiol., 3, 3-14 (1989).
8. Kanemori, Y., Gomi, K., Kitamoto, K., Kumagai, C., and Tamura, G., Insertion analysis of putative functional elements in the promoter region of the Aspergillus oryzae Taka-amylase A gene (amyB) using a heterologous Aspergillus nidulans amdS-lacZ fusion gene system. Biosci. Biotechnol. Biochem., 63, 180-183 (1999).
9. Minetoki, T., Nunokawa, Y., Gomi, K., Kitamoto, K., Kumagai, C., and Tamura, G., Deletion analysis of promoter elements of the Aspergillus oryzae agdA gene encoding α-glucosidase. Curr. Genet., 30, 432-438 (1996).
10. Gomi, K., Akeno, T., Minetoki, T., Ozeki, K., Kumagai, C., and Iimura, Y., Molecular cloning and characterization of a transcriptional activator gene, amyR, involved in the amylolytic gene expresssion in Aspergillus oryzae. Biosci. Biotechnol. Biochem., 64, 816-827 (2000).
11. Petersen, K. L., Lehmbeck, J., and Christensen, A new transcriptional activator for amylase genes in Aspergillus. Mol. Gen. Genet., 262, 668-676 (1999).
12. Tani, S., Kawaguchi, T., Kato, M., Kobayashi, T., and Tsukagoshi, N., A novel nuclear factor SREB binds to a cis-acting element SRE, required for inducible expression of the Aspergillus oryzae Takaamylase A Gene in A. nidulans. Mol. Gen. Genet., 263, 232-238 (2000).
13. Tani, S., Katsuyama, Y., Hayashi, T., Suzuki, H., Kato, M., Gomi, K., Kobayashi, T., and Tsukagoshi, N., Characterization of the amyR gene encoding a transcriptional activator for the amylase genes in Aspergillus nidulans. Curr. Genet., 39, 10-15 (2001).
14. Ballance, D. J. and Turner, G., Development of a high-frequency transforming vector for Aspergillus nidulans. Gene, 36, 321-331 (1985).
15. Rowlands, R. T. and Turner, G., Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans. Mol. Gen. Genet., 126, 201-206 (1973).
16. Lederberg, E. M. and Cohen, S. N., Transformation of Salmonella typhimurium by plasmid DNA. J. Bacteriol., 119, 1072-1074 (1974).
17. Higuchi, R. Recombinant PCR, ed. M. A. Innis, D. H. Gelfand, J. J. Sininsky and T. J. White, Academic Press, San Diego, pp. 177-183 (1990).
18. Kato, M., Sekine, K., and Tsukagoshi, N., Sequence-specific binding sites in the Taka-amylase A G2 promoter for the CreA represser mediating carbon catabolite repression. Biosci. Biotechnol. Biochem., 60, 1776-1779 (1996).
19. Sambrook, J., Fritsch, E. F., and Maniatis, T., "Molecular cloning: a laboratory manual," 2nd ed Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., (1989).
20. Nelson, N., A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem., 153, 375-380 (1944).
21. Chikamatsu, G., Shirai, K., Kato, M., Kobayashi, T., and Tsukagoshi, N., Structure and expression properties of the endo-β-1,4-glucanase A gene from a filamentous fungus Aspergillus nidulans. FEMS Microbiol. Lett., 179, 239-245 (1999).
22. 6 binuclear cluster DNA binding motif. Fungal Genetics and Biology, 21, 388-405 (1997).
23. 6" proteins distinguish between similar upstream activation sites? Comparison of the DNA-binding specificity of the GAL4 protein in vitro and in vivo. J. Biol. Chem., 268, 24699-24706 (1993).
24. Roy, A., Exinger, F., and Losson, R., cis- and transacting regulatory elements of the yeast URA3 promoter. Mol. Cell. Biol., 10, 5257-5270 (1990).
25. Zhang, L. and Guarente, L., The yeast activator HAP1 - a GAL4 family member - binds DNA in a directly repeated orientation. Genes Dev., 8, 2110-2119 (1994).
26. Nikolaev, I., Lenouvel, F., and Felenbok, B., Unique DNA binding specificity of the binuclear zinc AlcR activator of the ethanol utilization pathway in Aspergillus nidulans. J. Biol. Chem., 274, 9795-9802 (1999).
27. Marmorstein, R., Carey, M., Ptashne, M., and Harrison, S. C., DNA recognition by GAL4: structure of a protein-DNA complex [see comments]. Nature, 356, 408-414 (1992).
28. 6 binuclear cluster. Genes Dev., 8, 2504-2512 (1994).
29. King, D. A., Zhang, L., Guarente, L., and Marmorstein, R., Structure of a HAP1-DNA complex reveals dramatically asymmetric DNA binding by a homodimeric protein. Nat. Struct. Biol., 6, 64-71 (1999).