Azurin involved in alcohol oxidation system in Pseudomonas putida HK5: Expression analysis and gene cloning

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 7, 2001, Pages 1617-1626

  Toyama, Hirohide ^a   Aoki, Nahoko ^a   Matsushita, Kazunobu ^a   Adachi, Osao ^a  

^a YAMAGUCHI UNIVERSITY   (Japan)

Author keywords

Alcohol dehydrogenase; Azurin; PQQ; Pyrroloquinoline quinone; Quinoprotein

Indexed keywords

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS PUTIDA;

ALCOHOL DERIVATIVE; AZURIN; BACTERIAL DNA; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BINDING SITE; CHROMOSOME MAP; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION REACTION; PSEUDOMONAS PUTIDA; SEQUENCE HOMOLOGY;

ALCOHOLS; AMINO ACID SEQUENCE; AZURIN; BASE SEQUENCE; BINDING SITES; CHROMOSOME MAPPING; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0035403353     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.1617     Document Type: Article

References (34)

1. Adam, E. T., Copper protein structures. Advances in Protein Chemistry, 42, 145-197 (1991).
2. 551 and nitrite reductase. Eur. J. Biochem., 194, 109-118 (1990).
3. Edwards, S. L., Davidson, V. L., Hyun, Y.-L., and Wingfield, P. T., Spectroscopic evidence for a common electron transfer pathway for two tryptophan tryptophylquinone enzymes. J. Biol., Chem., 270, 4293-4298 (1995).
4. Causer, M. J., Hopper, D. J., McIntire, W. S., and Singer, T. P., Azurin from Pseudomonas putida: an acceptor for p-cresol methylhydroxylase. Biochem. Soc. Trans., 12, 1131-1132 (1984).
5. Duine, J. A., Electron transfer from bacterial dehydrogenases. In "From Neural Networks and Biomolecular Engineering to Bioelectronics", ed. Nicolini, C., Plenum, New York, pp. 87-94 (1995).
6. Gak, E. R., Chistoserdov, A. Y., and Lidstrom, M. E., Cloning, sequencing and mutation of a gene for azurin in Methylobacillus flagellatum KT. J. Bacteriol., 177, 4575-4578 (1995).
7. Toyama, H., Fujii, A., Matsushita, K., Shinagawa, E., Ameyama, M., and Adachi, O., Three distinct quinoprotein alcohol dehydrogenases are expressed when Pseudomonasputida is grown on different alcohols. J Bacteriol., 177, 2442-2450 (1995).
8. Matsushita, K., Yamashita, T., Aoki, N., Toyama, H., and Adachi, O., Electron transfer from quinohemoprotein alcohol dehydrogenase to blue copper protein azurin in the alcohol oxidase respiratory chain of Pseudomonas putida HK5. Biochemistry, 38, 6111-6118 (1999).
9. Vijgenboom, E., Busch, J. E., and Canters, G. W., In vivo studies disprove an obligatory role of azurin in denitrification in Pseudomonas aeruginosa and show that azu expression is under control of RpoS and ANR. Microbiology, 143, 2853-2863 (1997).
10. Canters, G. W., The azurin gene from Pseudomonas aeruginosa codes for a pre-protein with a signal peptide. FEBS Lett., 212, 167-172 (1987); Arvidsson, R. H. A., Nordling, M., and Lundberg, L. G., The azurin gene from Pseudomonas aeruginosa. Cloning and characterization. Eur. J. Biochem., 179, 195-200 (1989).
11. Canters, G. W., The azurin gene from Pseudomonas aeruginosa codes for a pre-protein with a signal peptide. FEBS Lett., 212, 167-172 (1987); Arvidsson, R. H. A., Nordling, M., and Lundberg, L. G., The azurin gene from Pseudomonas aeruginosa. Cloning and characterization. Eur. J. Biochem., 179, 195-200 (1989).
12. Hoitink, C. W. G., Woudt, L. P., Turenhout, J. C. M., van de Kamp, M., and Canters, G. W., Isolation and sequencing of the Alcaligenes denitrificans azurin encoding gene: comparison with the genes encoding blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis. Gene, 90, 15-20 (1990).
13. Taguchi, K., Kudo, T., and Tobari, J., Cloning and characterization of the azurin iso-1 gene, concerned with the electron transport chain involved in methylamine/methanol oxidation in the obligate methylotroph Methylomonas sp. strain J. Biosci. Biotechnol. Biochem., 62, 870-874 (1998); Taguchi, K., Kudo, T., Tobari, J., Genetic organization and characterization of the mau gene cluster, which concerned the initial step of electron transport chains involved in methylamine oxidation of the obligate methylotroph Methylomonas sp. strain J. J. Ferment. Bioeng., 83, 502-510 (1997); Ambler, R. P., and Tobari, J, Two distinct azurins function in the electron-transport chain of the obligate methylotroph Methylomonas J. Biochem. J., 261, 495-499 (1989).
14. Taguchi, K., Kudo, T., and Tobari, J., Cloning and characterization of the azurin iso-1 gene, concerned with the electron transport chain involved in methylamine/methanol oxidation in the obligate methylotroph Methylomonas sp. strain J. Biosci. Biotechnol. Biochem., 62, 870-874 (1998); Taguchi, K., Kudo, T., Tobari, J., Genetic organization and characterization of the mau gene cluster, which concerned the initial step of electron transport chains involved in methylamine oxidation of the obligate methylotroph Methylomonas sp. strain J. J. Ferment. Bioeng., 83, 502-510 (1997); Ambler, R. P., and Tobari, J, Two distinct azurins function in the electron-transport chain of the obligate methylotroph Methylomonas J. Biochem. J., 261, 495-499 (1989).
15. Taguchi, K., Kudo, T., and Tobari, J., Cloning and characterization of the azurin iso-1 gene, concerned with the electron transport chain involved in methylamine/methanol oxidation in the obligate methylotroph Methylomonas sp. strain J. Biosci. Biotechnol. Biochem., 62, 870-874 (1998); Taguchi, K., Kudo, T., Tobari, J., Genetic organization and characterization of the mau gene cluster, which concerned the initial step of electron transport chains involved in methylamine oxidation of the obligate methylotroph Methylomonas sp. strain J. J. Ferment. Bioeng., 83, 502-510 (1997); Ambler, R. P., and Tobari, J, Two distinct azurins function in the electron-transport chain of the obligate methylotroph Methylomonas J. Biochem. J., 261, 495-499 (1989).
16. Chen, Z. W., Barber, M. J., McIntire, W. S., and Mathews, F. S., Crystallographic study of azurin from Pseudomonas putida. Acta Cryst., D54, 253-268 (1998).
17. Matsushita, K., Yakushi, T., Toyama, H., Shinagawa, E., and Adachi, O., Function of multiple heme c moieties in intramolecular electron transport and ubiquinone reduction in the quinohemoprotein alcohol dehydrogenase-cytochrome c complex of Gluconobacter suboxydans. J. Biol. Chem., 271, 4850-4857 (1996).
18. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 227, 680-685 (1970).
19. Thomas, P. E., Ryan, D., and Levin, W., An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels. Anal. Biochem., 75, 168-176 (1976).
20. Dulley, J. R., and Grieve, P. A., A simple technique for estimation interference by detergents in the Lowry method of protein determination. Anal. Biochem., 64, 136-141 (1975).
21. Marmur, J., A procedure for the isolation of deoxynucleic acid from micro-organisms. J. Mol. Biol., 3, 208-218 (1961).
22. nd Ed., Cold Spring Harbar, N.Y. (1989).
23. Dodd, F. E., Hasmain, S. S., Hunter, W. N., Abraham, Z. H. L., Debenham, M., Kanzler, H., Eldridge, M., Eady, R. R., Ambler, R. P., and Smith, B. E., Evidence for two distinct azurins in Alcaligenes xylosoxidans (NCIMB 11015): Potential electron donors to nitrite reductase. Biochemistry, 34, 10180-10186 (1995).
24. Yamamoto, K., Uozumi, T., and Beppu, T., The blue copper protein gene of Alcaligenes faecalis S-6 directs secretion of blue copper protein from Escherichia coli cells. J. Bacteriol., 169, 5648-5652 (1987).
25. Leimg, Y.-C., Chan, C., Reader, J. S., Willis, A. C., van Spanning, R. J. M., Ferguson, S. J., and Radford,S. E., The pseudoazurin gene from Thiosphaera pantotropha: analysis of upstream putative regulatory sequences and overexpression in Escherichia coli. Biochem. J., 321, 699-705 (1997).
26. Yamanaka, T., Ota, A., and Okunuki, K., A nitrite reducing system reconstructed with purified cytochrome components of Pseudomonas aeruginosa. Biochim. Biophys. Acta, 53, 294-308 (1961).
27. 54-dependent regulators: derepression as a control mechanism,. Mol. Microbiol., 19, 409-416 (1996).
28. Stover, C. K., Pham, X. Q., Erwin, A. L., Mizoguchi, S. D., Warrener, P., Hickey, M. J., Brinkman, F. S., Hufnagle, W. O., Kowalik, D. J., Lagrou, M., Garber, R. L., Goltry, L., Tolentino, E., Westbrock-Wadman, S., Yuan, Y., Brody, L. L., Coulter, S. N., Folger, K. R., Kas, A., Larbig, K., Lim, R., Smith, K., Spencer, D., Wong, G. K., Wu, Z., and Paulsen, I. T., Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature, 406, 959-964 (2000).
29. Bauer, C. E., Elsen, S., and Bird, T. H., Mechanisms for redox control of gene expression. Ann. Rev. Microbiol., 53, 495-523 (1999).
30. Cotter, P. A., Chepuri, V., Gennis, R. B., and Gunsalus, R. P., Cytochrome o (cyoABCDE) and d (cydAB) oxidase gene expression in Escherichia coli is regulated by oxygen, pH and the fnr gene product. J. Bacteriol., 172, 6333-6338 (1990).
31. Spiro, S., Roberts, R. E., and Guest, J. R., FNR-dependent repression of ndh gene of Escherichia coli and metal requirement for FNR-regulated gene expression. Mol. Microbiol., 3, 601-608 (1989).
32. Winteler H. V., and Haas, D., The homologous regulators ANR of Pseudomonas aeruginosa and FNR of Escherichia coli have overlapping but distinct specificities for anaerobically inducible promoters. Microbiology, 142, 685-693 (1996).
33. Chen, Z. W., Baruch, P., Mathews, F. S., Matsushita, K., Yamashita, T., Toyama, H., and Adachi, O., Crystallization and preliminary diffraction studies of two quinoprotein alcohol dehydrogenases (ADHs): a soluble monomeric ADH from Pseudomonas putida HK5 (ADH-IIB) and a heterotrimeric membrane-bound ADH from Gluconobacter suboxydans (ADH-GS). Acta Crystallogr. D Biol. Crystallogr., 55, 1933-1936 (1999).
34. 6 and PQQ-dependent proteins", eds. Iriarte, A., Kagan, H. M., and Martinez-Carrion, M., Birkhauser, Basel-Boston-Berlin, pp. 219-224 (2000).