An immunohistochemical method for the detection of proteins in the vertebrate lens

Journal of Immunological Methods

Volumn 253, Issue 1-2, 2001, Pages 243-252

  Reed, Nathan A ^a   Oh, Dong Jin ^a   Czymmek, Kirk J ^a   Duncan, Melinda K ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

Confocal microscopy; Embryo; Immunohistochemistry; Lens epithelium; Lens fibers; Vertebrate lens

Indexed keywords

ANTIGEN;

ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; BASEMENT MEMBRANE; CELL MEMBRANE; CELL NUCLEUS; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EMBRYO; ENDOPLASMIC RETICULUM; FLUORESCENCE HISTOCHEMISTRY; HUMAN; HUMAN TISSUE; LENS CAPSULE; LENS EPITHELIUM; LIVER; MORPHOLOGY; MOUSE; MUSCLE TISSUE; NONHUMAN; PANCREAS; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN LOCALIZATION; RAT; TISSUE PREPARATION; VERTEBRATE;

ANIMALS; ANIMALS, NEWBORN; EYE PROTEINS; IMMUNOHISTOCHEMISTRY; LENS, CRYSTALLINE; MICE; MICROSCOPY, CONFOCAL; MICROTOMY; RATS; RATS, LONG-EVANS; TISSUE EMBEDDING; VERTEBRATES;

VERTEBRATA;

EID: 0035400353     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(01)00374-X     Document Type: Article

References (8)

1. Chromatin degradation in differentiating fiber cells of the eye lens
2. Targeted disruption of the mouse alpha A-crystallin gene induces cataract and cytoplasmic inclusion bodies containing the small heat shock protein alpha B-crystallin
3. Developmental regulation of the chicken beta B1-crystallin promoter in transgenic mice
4. Truncated forms of Pax-6 disrupt lens morphology in transgenic mice
5. Over expression of PAX6(5A) in lens fiber cells results in cataract and upregulation of α5β1 integrin expression
6. A simple method of reducing the fading of immunofluorescence during microscopy
7. Influence of specific regions in Lp82 calpain on protein stability, activity, and localization within lens
8. Pax-6 is essential for lens-specific expression of zeta-crystallin