Simultaneous flow cytometric measurement of K-562 megakaryocytic differentiation and CD56 + large granular lymphocyte cytotoxicity

Journal of Immunological Methods

Volumn 253, Issue 1-2, 2001, Pages 37-44

  Baker, E J ^a   Ichiki, A T ^a   Day, N E ^a   Andrews, R B ^a   Bamberger, E G ^a   Lozzio, C B ^a  

^a UNIVERSITY OF TENNESSEE MEDICAL CENTER   (United States)

Author keywords

Flow cytometry; Human CD56 cells; Human CD61; K 562; Phorbol ester

Indexed keywords

BETA3 INTEGRIN; CD56 ANTIGEN; CD61 ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; PHORBOL 13 ACETATE 12 MYRISTATE;

ANTIGEN BINDING; ANTIGEN EXPRESSION; ARTICLE; CELL DEATH; CELL DIFFERENTIATION; CELL STRAIN K 562; CELL VIABILITY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LARGE GRANULAR LYMPHOCYTE; LYMPHOCYTOTOXICITY; MAJOR HISTOCOMPATIBILITY COMPLEX; MEGAKARYOCYTE; PHENOTYPE; PRIORITY JOURNAL; TARGET CELL;

ANTIGENS, CD; ANTIGENS, CD56; CELL DEATH; CELL DIFFERENTIATION; COLOR; CYTOTOXICITY TESTS, IMMUNOLOGIC; FLOW CYTOMETRY; HUMANS; INTEGRIN BETA3; K562 CELLS; KILLER CELLS; KINETICS; LEUKEMIA, MYELOID, CHRONIC; MEGAKARYOCYTES; PLATELET MEMBRANE GLYCOPROTEINS; TETRADECANOYLPHORBOL ACETATE;

EID: 0035400282     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(01)00373-8     Document Type: Article

References (23)

1. Induced differentiation of K562 leukemia cells: A model for studies of gene expression in early megakaryoblasts
2. Enumeration of lymphocyte-target cell conjugates by cytofluorometry
3. An improved double fluorescence flow cytometry method for the quantification of killer cell/target cell conjugate formation
4. Variation amongst K562 cell cultures
5. K562 cells induced to differentiate by phorbol ester tumor promotors resist NK lysis
6. Natural killer cell resistance in K-562 cell sublines
7. Induction of hemoglobin synthesis in original K 562 cell line
8. Population distributions of natural killer and K562 target cell conjugates
9. Protective effects of differentiation inducers on natural killer sensitivity of K 562 cells: Analysis at a single-cell level
10. Diversity of cell surface hematopoietic antigens on K-562 sublines identified with monoclonal antibodies
11. Differential regulation of interleukin-1alpha and interleukin-1beta in K-562 cells
12. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome
13. Flow cytometric measurement of NK cell immunoconjugates by pulse width processing
14. Natural killer function in flow cytometry, I. Evaluation of NK lytic activity on K562 cell line
15. Effects of protein kinase C (PK-C) activators and inhibitors on human large granular lymphocytes (LGL): Role of PK-C on natural killer (NK) activity
16. The measurement of specific cell: Cell interactions by dual-parameter flow cytometry
17. The application of flow cytometry in the study of natural killer cell cytotoxicity
18. Megakaryoblastic differentiation of proerythroblastic K562 cell-line cells
19. Biology of natural killer cells
20. Natural killer function in flow cytometry, II. Evaluation of NK lytic activity by means of target cell morphological changes detected by right angle light scatter
21. Natural killer function in flow cytometry: Identification of human lymphoid subsets able to bind to the NK sensitive target K562
22. Natural killer function in flow cytometry, III. Surface marker determination of K562-conjugated lymphocytes by dual laser flow cytometry
23. Kinetics of in vitro natural killer activity against K562 cells as detected by flow cytometry