Gene expression analysis of an H2O2-resistant lens epithelial cell line

Free Radical Biology and Medicine

Volumn 31, Issue 1, 2001, Pages 90-97

  Carper, Deborah ^a   John, Molykutty ^a   Chen, Zhengguang ^a   Subramanian, Saradha ^a   Wang, Ren Rong ^b   Ma, Wanchao ^b   Spector, Abraham ^b  

^a NATIONAL EYE INSTITUTE   (United States)

^b Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

Differential display; Free radicals; Gene expression; Hydrogen peroxide; Lens cell line; RT PCR

Indexed keywords

ALDEHYDE REDUCTASE; ALPHA CRYSTALLIN; ANTIOXIDANT; CALCIUM BINDING PROTEIN; CATALASE; CELL ENZYME; CHAPERONE; FERRITIN; GENE PRODUCT; GLUTATHIONE PEROXIDASE; HYDROGEN PEROXIDE; MESSENGER RNA; METAL; RETICULOCALBIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIOXIDANT ACTIVITY; ARTICLE; CELL PROTECTION; CELL SURVIVAL; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME REGULATION; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; LENS EPITHELIUM; MOUSE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; REGULATORY MECHANISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

ANIMALS; CALCIUM-BINDING PROTEINS; CATALASE; CELL LINE; CRYSTALLINS; DNA PRIMERS; DRUG RESISTANCE; EPITHELIAL CELLS; FERRITINS; GENE EXPRESSION; GENE EXPRESSION PROFILING; GLUTATHIONE PEROXIDASE; HYDROGEN PEROXIDE; LENS, CRYSTALLINE; MICE; OXIDATIVE STRESS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; UP-REGULATION;

EID: 0035399869     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(01)00561-5     Document Type: Article

References (62)

1. Oxidative stress-induced cataract: Mechanism of action
2. A forty-two year voyage through vision research
3. Glutathione: A vital lens antioxidant
4. Sulfur oxidation in selected human cortical cataracts and nuclear cataracts
5. Quantitation of high molecular weight protein aggregates in opaque and transparent parts from the same human cataractous lens
6. Hydrogen peroxide and human cataract
7. Molecular mechanism of cataractogenesis: III. Toxic metabolites of oxygen as initiators of lipid peroxidation and cataract
8. Radio-isotopic determination of hydrogen peroxide in aqueous humor and urine
9. Effect of chronic near-ultraviolet radiation on the gray squirrel lens in vivo
10. Effects of UVA light in vivo and in vitro on lenses of the guinea pig and rabbit
11. Ultraviolet radiation and cataract
12. The optical properties of the anterior segment of the eye: Implications for cortical cataract
13. Cross-linking of lens crystallins in a photodynamic system: A process mediated by singlet oxygen
14. The generation of superoxide anion by the UVA irradiation of human lens proteins
15. Superoxide dismutase of the eye, relative functions of superoxide dismutase and catalase in protecting the ocular lens from oxidative damage
16. A brief photochemically induced oxidative insult causes irreversible lens damage and cataract. I. Transparency and epithelial cell layer
17. A brief photochemically induced oxidative insult causes irreversible lens damage and cataract. II. Mechanism of action
18. The effects of hyperbaric oxygen on the crystallins of cultured rabbit lenses: A possible catalytic role for copper
19. The role of the lens epithelium in development of UV cataract
20. Lens epithelium: A primary target of UVB irradiation
21. 2-resistant immortal lens epithelial cell line
22. Oxidative stress induces differential gene expression in a human lens epithelial cell line
23. Differential display analysis of hydrogen peroxide resistant αTN4 mouse lens epithelial cells
24. 2 conditioned cells
25. Amizol-induced cataract and inhibition of lens catalase in rabbit
26. The effect of catalase amplification on immortal lens epithelial cell lines
27. Characteristics of catalase transgenics controlled by a lens specific promoter
28. Peroxide-induced damage in lenses of transgenic mice with deficient and elevated levels of glutathione peroxidase
29. The effect of photochemical stress upon the lenses of normal and glutathione peroxidase-1 knockout mice
30. Differential display detects altered gene expression between cataractous and normal human lenses
31. Oxygen toxicity, oxygen radicals, transition metals and disease
32. Iron detoxifying activity of ferritin: Effects of H and L human apoferritins on lipid peroxidation in vitro
33. The effect of ascorbic acid and ferric ammonium citrate on iron uptake and storage in lens epithelial cells
34. Structural changes in bovine lens crystallins induced by ascorbate, metal, and oxygen
35. 2 and chelated iron
36. Prevention of hepatocyte injury and lipid peroxidation by iron chelators and alpha-tocopherol in isolated iron-loaded rat hepatocytes
37. Enhanced γ-glutamyl transpeptidase expression and selective loss of CuZn superoxide dismutase in hepatic iron overload
38. 2+-binding protein with multiple EF-hand motifs and a carboxyl-terminal HDEL sequence
39. The reticulocalbin gene maps to the WAGR region in human and to the Small Eye Harwell deletion in mouse
40. Differential display of reticulocalbin in the highly invasive cell, MDA-MB-435, versus the poorly invasive cell line, MCF-7
41. α-Crystallin can function as a molecular chaperone
42. Alpha crystallin prevents irreversible protein denaturation and acts cooperatively with other heat-shock proteins to renature the stabilized partially denatured protein in an ATP-dependent manner
43. Immunoreactive alpha A crystallin in rat non-lenticular tissues detected with a sensitive immunoassay method
44. αA-crystallin is expressed in non-ocular tissues
45. αB subunit of lens-specific protein α-crystallin is present in other ocular and non-ocular tissues
46. Expression of the murine αB-crystallin gene is not restricted to the lens
47. αB-crystallin is expressed in non-lenticular tissues and accumulates in Alexander's disease brain
48. Alpha B-crystallin is a small heat shock protein
49. Hypertonic stress induces αB-crystallin expression
50. Coordinate and independent regulation of αB-crystallin and HSP27 expression in response to physiological stress
51. The molecular chaperone alphaB-crystallin enhances amyloid beta neurotoxicity
52. Downregulation of mammalian mitochondrial RNAs during oxidative stress
53. Characterization of an oxygen-tolerant cell line derived from chinese hamster ovary. Antioxygenic enzyme levels and ultrastructural morphometry of peroxisomes and mitochondria
54. 2-resistant chinese hamster fibroblast cell lines
55. Amplification and hyperexpression of the catalase gene in selenoperoxidase-deficient leukemia cells
56. Expression of catalase and myeloperoxidase genes in hydrogen peroxide-resistant HL-60 cells
57. Genomic instability and catalase gene amplification induced by chronic exposure in oxidative stress
58. 2-resistant fibroblasts
59. L in peroxide-resistant A549 and U87MG cells
60. Some characteristics of hyperoxia-adapted HeLA cells. A tissue culture model for cellular oxygen tolerance
61. 2 plus tertiary butyl hydroperoxide