Presteady-state kinetics of Bacillus 1,3-1,4-β-glucanase: Binding and hydrolysis of a 4-methylumbelliferyl trisaccharide substrate

Biochemical Journal

Volumn 357, Issue 1, 2001, Pages 195-202

  Abel, M ^a   Planas, A ^a   Christensen, U ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Fluorescence; Induced fit; Inhibition; Mechanism; Stopped flow

Indexed keywords

BINDING ENERGY; CATALYSIS; ENZYME KINETICS; FLUORESCENCE; HYDROLYSIS; MUTAGENESIS; RATE CONSTANTS;

MUTANTS;

BIOCHEMISTRY;

4 METHYLUMBELLIFERYL 3 O BETA CELLOBIOSYL BETA DEXTRO GLUCOSIDE; HYMECROMONE; TRISACCHARIDE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BACTERIAL METABOLISM; COMPLEX FORMATION; ENZYME BINDING; ENZYME KINETICS; ENZYME SUBSTRATE; GLYCOSYLATION; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; STEADY STATE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SUBSTITUTION; BACILLUS; GLYCOSIDE HYDROLASES; HYDROLYSIS; KINETICS; MODELS, THEORETICAL; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS);

EID: 0035395783     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3570195     Document Type: Article

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