Purification and characterization of an oligo-isomaltosaccharide synthase from a Streptococcus sobrinus glucosyltransferase-I deficient mutant

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 6, 2001, Pages 1290-1295

  Shinozaki Kuwahara, Noriko ^a   Hayakawa, Mitsuo ^a   Shiroza, Teruaki ^a   Abiko, Yoshimitsu ^a   Fukushima, Kazuo ^a  

^a NIHON UNIVERSITY   (Japan)

Author keywords

Glucosyltransferase; Oligo isomaltosaccharide; Streptococcus sobrinus

Indexed keywords

ORYCTOLAGUS CUNICULUS; STREPTOCOCCUS SOBRINUS;

GLUCAN; GLUCOSYLTRANSFERASE;

ARTICLE; CHEMISTRY; CULTURE MEDIUM; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; STREPTOCOCCUS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLUCANS; GLUCOSYLTRANSFERASES; MUTATION; STREPTOCOCCUS;

EID: 0035376165     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.1290     Document Type: Article

References (26)

1. Hamada, S., and Slade H. D., Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol. Rev., 44, 331-384 (1980).
2. Loesche, W. J., Role of Streptococcus mutans in human dental decay. Microbiol. Rev., 50, 353-380 (1986).
3. Hirose, H., Hirose, K., Isogai, E., Miura, H., and Ueda, I., Close association between Streptococcus sobrinus in the saliva of young children and smooth-surface caries increment. Caries Res., 27, 292-297 (1993).
4. Shimamura, A., Tsumori, H., and Mukasa, H., Three kinds of extracellular glucosyltransferases from Streptococcus mutans 6715 (serotype g). FEBS Lett., 157, 79-84 (1983).
5. Namiki, Y., Kantake, I., Ikeda, T., and Fukushima, K., Separation of three kinds of glucosyltransferase from Streptococcus mutans strain B13. IRCS. Med. Sci., 13, 501-502 (1985).
6. Yamashita, Y., Hanada, N., and Takehara, T., A novel glucosyltransferase from Streptococcus mutons produces oligo-isomaltosaccharides. Biochem. Biophys. Res. Com., 150, 687-693 (1988).
7. Russell, R. R. B., The application of molecular genetics to the microbiology of dental caries. Caries Res. 28, 69-82 (1994).
8. Fukushima, K., Motoda, R., Takada, K., and Ikeda, T., Resolution of Streptococcus mutons glucosyltransferases into two components essential to water-insoluble glucan synthesis. FEBS Lett., 128, 213-216 (1981).
9. Fukushima, K., Takada, K., Motoda, R., and Ikeda, T., Independence of water-insoluble glucan synthesis and adherence of Streptococcus mutans to smooth surfaces. FEBS Lett., 149, 299-303 (1982).
10. Inoue, M., Koga, T., Sato, S., and Hamada, S., Synthesis of adherent insoluble glucan by the concerted action of the two glucosyltransferase components of Streptococcus mutans. FEBS Lett. 143, 101-104 (1982).
11. Gilmore, K. S., Russell, R. R. B., and Ferretti, J. J., Analysis of the Streptococcus downei gtfS gene, which specifies a glucosyltransferase that synthesizes soluble glucans. Infect. Immun. 58, 2452-2458 (1990).
12. Hayakawa, M., Fukushima, K., Abiko, Y., Ikeda, T., and Takiguchi, H., Cloning of a Streptococcus sobrinus oligo-isomaltosaccharide synthase gene and characterization of its product. Biochem. Mol. Biol. Int., 31, 1167-1175 (1993).
13. Russell R. R. B, Gilpin, M. L., Hanada, N., Yamashita, Y., Shibata, Y., and Takehara T., Characterization of the product of the gtfS gene of Streptococcus downei, a primer-independent enzyme synthesizing oligo-isomaltosaccharides. J. Gen. Microbiol., 136, 1631-1637 (1990).
14. Fukushima, K., Motoda, R., and Ikeda, T., Effects of exogenous insoluble glucan primer on insoluble glucan synthesis by Streptococcus mutans. J. Dent. Res., 60, 1707-1712 (1981).
15. Namiki, Y., Kantake, I., and Fukushima, K., In vitro cariogenicity of α-1,3-glucan synthase deficient mutants of Streptococcus mutans. Japan. J. Conservative Dentistry (in Japanese), 28, 608-615 (1985).
16. Somogyi, M., Notes on sugar determination. J. Biol. Chem., 195, 19-23 (1952).
17. Weber, K., and Osborn, M., The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem., 244, 4406-4412 (1969).
18. Kapitany, R. A., and Zebrowski, E. J., A high resolution PAS stain for polyacrylamide gel electrophoresis. Anal. Biochem., 56, 361-369 (1973).
19. Towbin, H., Staehelin, T., and Gordon, J., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Pro. Natl. Acad. Sci. USA., 76, 4350-4354 (1979).
20. Nanbu, A., Hayakawa, M., Takada, K., Shinozaki, N., Abiko, Y., and Fukushima, K., Production, characterization, and application of monoclonal antibodies which distinguish four glucosyltransferases from Streptococcus sobrinus. FEMS Immun. Med. Microbiol., 27, 9-15 (2000).
21. Fujiwara, T., Tamesada, M., Bian, Z., Kawabata, S., Kimura, S., and Hamada, S., Deletion and reintroduction of glucosyltransferase genes of Streptococcus mutans and role of their gene products in sucrose dependent cellular adherence. Microb. Pathog., 20, 225-233 (1996).
22. Kuramitsu, H. K., Virulence factors of mutans streptococci: Role of molecular genetics. Crit. Rev. Oral Biol. Med., 4 159-176 (1993).
23. Gilmore, K. S., Russell, R. R. B., and Ferretti, J. J., Expression of gtfS is essential for normal insoluble glucan synthesis by Streptococcus downei. Infect. Immun., 61, 1246-1250 (1993).
24. Colby, S. M., McLaughlin, R. E., Ferretti, J. J., and Russell, R. R. B., Effect of inactivation of gtf genes on adherence of Streptococcus downei. Oral Microbiol. Immunol., 14, 27-32 (1999).
25. Yamashita, Y., Hanada, N., and Takehara, T., Purification of a fourth glucosyltransferase from Streptococcus sobrinus. J. Bacteriol., 171, 6265-6270 (1989).
26. Koga, T., Sato, S., Inoue, M., Takeuchi, K., Furuta, T., and Hamada, S., Role of primers in glucan synthesis by glucosyltransferases from Streptococcus mutans strain OMZ176. J. Gen. Microbiol., 129, 751-754 (1983).