Optimisation of an ELISA for the serodiagnosis of visceral leishmaniasis using in vitro derived promastigote antigens

Journal of Immunological Methods

Volumn 252, Issue 1-2, 2001, Pages 105-119

  Rajasekariah, G Halli R ^a   Ryan, Jeffrey R ^b   Hillier, Scott R ^a   Yi, Lisa P ^b   Stiteler, John M ^b   Cui, Liwang ^b   Smithyman, Anthony M ^a   Martin, Samuel K ^b  

^a Cellabs Pty Ltd   (Australia)

^b WALTER REED ARMY INSTITUTE OF RESEARCH   (United States)

Author keywords

Anti human HRP conjugate; ELISA; Endemic sera; ESM antigen; Field ELISA; Leishmania donovani; Promastigote; Signal noise ratio; Specific antibody reactivity; Visceral leishmaniasis

Indexed keywords

ANTIBODY CONJUGATE; CASEIN; CHROMOGENIC SUBSTRATE; MONOCLONAL ANTIBODY; PARASITE ANTIGEN;

ANTIBODY DETECTION; ANTIGEN ANTIBODY REACTION; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; LEISHMANIA DONOVANI; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROMASTIGOTE; SERODIAGNOSIS; VISCERAL LEISHMANIASIS;

ANIMALS; ANTIBODIES, PROTOZOAN; ANTIGENS, PROTOZOAN; BUFFERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; LEISHMANIA DONOVANI; LEISHMANIASIS, VISCERAL; SEROLOGIC TESTS; SODIUM DODECYL SULFATE; SOLUTIONS;

LEISHMANIA DONOVANI; NUCLEOPOLYHEDROVIRUS;

EID: 0035371663     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(01)00341-6     Document Type: Article

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