A simple and highly efficient fixation method for Chrysochromulina polylepis (Prymnesiophytes) for analytical flow cytometry

Cytometry

Volumn 44, Issue 2, 2001, Pages 126-132

  Shapiro, Howard M ^a,b   Perlmutter, Nancy G ^a  

^a ISAC XX ^*  (United States)

^b ISAC XX   (France)

Author keywords

Aldehydes; Chrysochromulina polylepis; Fixation; Flow cytometry; Glutaraldehyde

Indexed keywords

ALDEHYDE; GLUTARALDEHYDE; METHANOL;

ALGA; ARTICLE; CELL DENSITY; CENTRIFUGATION; CHRYSOCHROMULINA POLYLEPIS; CONTROLLED STUDY; FLOW CYTOMETRY; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY; PRYMNESIOPHYTE; STORAGE;

ALDEHYDES; ALGAE; FLOW CYTOMETRY; METHANOL; TISSUE FIXATION;

EID: 0035371272     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20010601)44:2<126::AID-CYTO1091>3.0.CO;2-N     Document Type: Article

References (28)

1. Species-specific phytoplankton growth rates via diel DNA synthesis cycles. II. DNA quantification and model verification in the dinoflagellate Heterocapsa triquetra
2. Cytometric measurement of the DNA cell cycle in the presence of chlorophyll autofluorescence in marine eukaryotic phytoplankton by the blue-light excited dye YOYO-1
3. Species-specific phytoplankton growth rates via diel DNA synthesis cycles. V. Application to natural populations in Long Island Sound
4. In situ growth rates of Emiliania huxleyi in enclosures with different phosphate loadings revealed by diel changes in DNA content
5. Growth of Prochlorococcus, a photosynthetic prokaryote in the equatorial Pacific Ocean
6. Flow cytometric determination of cell cycles and growth rates of Prorocentrum spp
7. Sinking rates of marine phytoplankton measured with a fluorometer
8. The Chrysochromulina polylepis bloom in Scandinavian waters during spring 1988
9. Long-term preservation of picophytoplankton for counting by fluorescence microscopy
10. A simple method to preserve oceanic phytoplankton for flow cytometric analyses
11. Ultraphytoplankton in the eastern Mediterranean Sea: Towards deriving phytoplankton biomass from flow cytometric measurements of abundance, fluorescence and light scatter
12. Quantitative enumeration of paraformaldehyde preserved Prochlorococcus by flow cytometry
13. Flow cytometric analysis of coastal lagoon bacterioplankton and picophytoplankton: Fixation and storage effects
14. Flow cytometric analysis of marine bacteria with Hoechst 33342
15. A note on losses of Prochlorococcus cells during preservation
16. Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1 and PicoGreen for flow cytometric analyses of marine prokaryotes
17. Picoplankton population dynamics in coastal waters of the northwestern Mediterranean Sea
18. Photosynthetic picoplankton community structure in the subtropical North Pacific Ocean near Hawaii (station ALOHA)
19. Photosynthetic picoplankton in French Polynesian Atoll lagunes: Estimation of taxa contribution to biomass and production by flow cytometry
20. Prochlorococcus and Synechococcus growth rates and contributions to production in the Arabian Sea during the 1995 Southwest and Northeast monsoons
21. Characterization of oceanic photosynthetic picoeukaryotes by flow cytometry
22. Cellular DNA content of marine phytoplankton using two new fluorochromes: Taxonomic and ecological implications
23. The chemical fixation of biological materials for electron microscopy
24. The current status of fixation for electron microscopy: A review