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1
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0034708480
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Thegenome sequence of Drosophila melanogaster
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Reports the sequencing of most of the ~120 Mb euchromatic portion of the Drosophila genome and describes its prominent features. A total of 13 601 genes (including previously known genes) were identified using computational methods. This paper also surveys genes known to be involved in basic cellular processes including DNA replication, metabolism and solute transport.
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Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., et al. Thegenome sequence of Drosophila melanogaster. Science. 287:2000;2185-2195. Reports the sequencing of most of the ~120 Mb euchromatic portion of the Drosophila genome and describes its prominent features. A total of 13 601 genes (including previously known genes) were identified using computational methods. This paper also surveys genes known to be involved in basic cellular processes including DNA replication, metabolism and solute transport.
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(2000)
Science
, vol.287
, pp. 2185-2195
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Adams, M.D.1
Celniker, S.E.2
Holt, R.A.3
Evans, C.A.4
Gocayne, J.D.5
Amanatides, P.G.6
Scherer, S.E.7
Li, P.W.8
Hoskins, R.A.9
Galle, R.F.10
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2
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0034708444
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Comparative genomics of the eukaryotes
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The proteome of Drosophila is compared to that of the nematode Caenorhabditis elegans and the yeast Saccharomyces cerevisiae. Particular attention is paid to regulators of the cytoskeleton, the cell cycle, apoptosis, cell adhesion and cell signalling. Genes encoding components of the nervous system and the immune system are analyzed. Among 289 selected human disease genes, 62% were found to have Drosophila orthologues.
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Rubin G.M., Yandell M.D., Wortman J.R., Gabor Miklos G.L., Nelson C.R., Hariharan I.K., Fortini M.E., Li P.W., Apweiler R., Fleischmann W., et al. Comparative genomics of the eukaryotes. Science. 287:2000;2204-2215. The proteome of Drosophila is compared to that of the nematode Caenorhabditis elegans and the yeast Saccharomyces cerevisiae. Particular attention is paid to regulators of the cytoskeleton, the cell cycle, apoptosis, cell adhesion and cell signalling. Genes encoding components of the nervous system and the immune system are analyzed. Among 289 selected human disease genes, 62% were found to have Drosophila orthologues.
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(2000)
Science
, vol.287
, pp. 2204-2215
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Rubin, G.M.1
Yandell, M.D.2
Wortman, J.R.3
Gabor Miklos, G.L.4
Nelson, C.R.5
Hariharan, I.K.6
Fortini, M.E.7
Li, P.W.8
Apweiler, R.9
Fleischmann, W.10
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3
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0033668724
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The Drosophila genome
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A succinct summary of the methods used to sequence the Drosophila genome, and of the main findings and their implications.
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Celniker S.E. The Drosophila genome. Curr Opin Genet Dev. 10:2000;612-616. A succinct summary of the methods used to sequence the Drosophila genome, and of the main findings and their implications.
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(2000)
Curr Opin Genet Dev
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Celniker, S.E.1
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4
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0030294408
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Mutations in the human Sonic Hedgehog gene cause holoprosencephaly
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Roessler E., Belloni E., Gaudenz K., Jay P., Berta P., Scherer S.W., TsuiL C., Muenke M. Mutations in the human Sonic Hedgehog gene cause holoprosencephaly. Nat Genet. 14:1996;357-360.
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Nat Genet
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Roessler, E.1
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Berta, P.5
Scherer, S.W.6
Tsuil, C.7
Muenke, M.8
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5
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0031046284
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A human homologue of the Drosophila eyes absent gene underlies branchio- oto-renal (BOR) syndrome and identifies a novel gene family
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Abdelhak S., Kalatzis V., Heilig R., Compain S., Samson D., Vincent C., Weil D., Cruaud C., Sahly I., Leibovici M., et al. A human homologue of the Drosophila eyes absent gene underlies branchio- oto-renal (BOR) syndrome and identifies a novel gene family. Nat Genet. 15:1997;157-164.
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Nat Genet
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Abdelhak, S.1
Kalatzis, V.2
Heilig, R.3
Compain, S.4
Samson, D.5
Vincent, C.6
Weil, D.7
Cruaud, C.8
Sahly, I.9
Leibovici, M.10
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6
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0031021336
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Mutations in TWIST, a basic helix-loop-helix transcription factor, in Saethre-Chotzen syndrome
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Howard T.D., Paznekas W.A., Green E.D., Chiang L.C., Ma N., OrtizdeLuna R.I., Garcia Delgado C., Gonzalez-Ramos M., Kline A.D., Jabs E.W. Mutations in TWIST, a basic helix-loop-helix transcription factor, in Saethre-Chotzen syndrome. Nat Genet. 15:1997;36-41.
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Nat Genet
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Howard, T.D.1
Paznekas, W.A.2
Green, E.D.3
Chiang, L.C.4
Ma, N.5
Ortizdeluna, R.I.6
Garcia Delgado, C.7
Gonzalez-Ramos, M.8
Kline, A.D.9
Jabs, E.W.10
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7
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0031012353
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Mutations of the TWIST gene in the Saethre-Chotzen syndrome
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el Ghouzzi V, Le Merrer M, Perrin-Schmitt F, Lajeunie E, Benit P, RenierD, Bourgeois P, Bolcato-Bellemin AL, Munnich A, Bonaventure J: Mutations of the TWIST gene in the Saethre-Chotzen syndrome. Nat Genet 1997, 15:42-46.
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Nat Genet
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El Ghouzzi, V.1
Le Merrer, M.2
Perrin-Schmitt, F.3
Lajeunie, E.4
Benit, P.5
Renier, D.6
Bourgeois, P.7
Bolcato-Bellemin, A.L.8
Munnich, A.9
Bonaventure, J.10
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8
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16044362074
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Notch3 mutations in CADASIL, a hereditary adult-onset condition causing stroke and dementia
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Joutel A., Corpechot C., Ducros A., Vahedi K., Chabriat H., Mouton P., Alamowitch S., Domenga V., Cecillion M., Marechal E., et al. Notch3 mutations in CADASIL, a hereditary adult-onset condition causing stroke and dementia. Nature. 383:1996;707-710.
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Joutel, A.1
Corpechot, C.2
Ducros, A.3
Vahedi, K.4
Chabriat, H.5
Mouton, P.6
Alamowitch, S.7
Domenga, V.8
Cecillion, M.9
Marechal, E.10
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9
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0030996903
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Rescue of a Drosophila NF1 mutant phenotype by protein kinase A
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The I., Hannigan G.E., Cowley G.S., Reginald S., Zhong Y., Gusella J.F., Hariharan I.K., Bernards A. Rescue of a Drosophila NF1 mutant phenotype by protein kinase A. Science. 276:1997;791-794.
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(1997)
Science
, vol.276
, pp. 791-794
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The, I.1
Hannigan, G.E.2
Cowley, G.S.3
Reginald, S.4
Zhong, Y.5
Gusella, J.F.6
Hariharan, I.K.7
Bernards, A.8
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10
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0031007708
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Requirement of Drosophila NF1 for activation of adenylyl cyclase by PACAP38-like neuropeptides
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Guo H.F., The I., Hannan F., Bernards A., Zhong Y. Requirement of Drosophila NF1 for activation of adenylyl cyclase by PACAP38-like neuropeptides. Science. 276:1997;795-798.
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Science
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Guo, H.F.1
The, I.2
Hannan, F.3
Bernards, A.4
Zhong, Y.5
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11
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0034708076
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A neurofibromatosis-1- regulated pathway is required for learning in Drosophila
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This and the two preceding papers 9, 10 show that several Drosophila NF1 null mutant phenotypes are insensitive to genetic manipulation of Ras signalling, but rescued by increasing signaling through the adenylyl cyclase/protein kinase A pathway. A role for NF1 in cAMP-mediated learning reported in this paper is intriguing, as an estimated 40% of children with NF1 exhibit a cognitive defect.
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Guo H.F., Tong J., Hannan F., Luo L., Zhong Y. A neurofibromatosis-1- regulated pathway is required for learning in Drosophila. Nature. 403:2000;895-898. This and the two preceding papers 9, 10 show that several Drosophila NF1 null mutant phenotypes are insensitive to genetic manipulation of Ras signalling, but rescued by increasing signaling through the adenylyl cyclase/protein kinase A pathway. A role for NF1 in cAMP-mediated learning reported in this paper is intriguing, as an estimated 40% of children with NF1 exhibit a cognitive defect.
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(2000)
Nature
, vol.403
, pp. 895-898
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Guo, H.F.1
Tong, J.2
Hannan, F.3
Luo, L.4
Zhong, Y.5
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12
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0034710151
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A survey of human disease gene counterparts in the Drosophila genome
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Details of the study described in 2•• that analyzed the Drosophila genome for orthologues of 289 human disease genes. Some of the new disease gene orthologues found by the sequencing effort, including menin and STK11, are highlighted.
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Fortini M.E., Skupski M.P., Boguski M.S., Hariharan I.K. A survey of human disease gene counterparts in the Drosophila genome. JCell Biol. 150:2000;F23-F30. Details of the study described in 2•• that analyzed the Drosophila genome for orthologues of 289 human disease genes. Some of the new disease gene orthologues found by the sequencing effort, including menin and STK11, are highlighted.
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(2000)
JCell Biol
, vol.150
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Fortini, M.E.1
Skupski, M.P.2
Boguski, M.S.3
Hariharan, I.K.4
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13
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20244385424
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Drosophila p53 is a structural and functional homolog of the tumor suppressor p53
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1 arrest, whereas expression of dominant negative Dmp53 transgenes blocked X-ray-induced apoptosis, but not cell-cycle arrest. These findings reveal an ancestral role for Dmp53 in apoptosis.
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1 arrest, whereas expression of dominant negative Dmp53 transgenes blocked X-ray-induced apoptosis, but not cell-cycle arrest. These findings reveal an ancestral role for Dmp53 in apoptosis.
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(2000)
Cell
, vol.101
, pp. 91-101
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Ollmann, M.1
Young, L.M.2
Di Como, C.J.3
Karim, F.4
Belvin, M.5
Robertson, S.6
Whittaker, K.7
Demsky, M.8
Fisher, W.W.9
Buchman, A.10
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14
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0034737292
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Drosophila p53 binds a damage response element at the reaper locus
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Results reported in this paper indicate that the proapoptotic reaper gene is a direct transcriptional target of Drosophila p53 following DNA damage. This defines a mechanism by which p53 induces apoptosis in Drosophila cells.
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Brodsky M.H., Nordstrom W., Tsang G., Kwan E., Rubin G.M., Abrams J.M. Drosophila p53 binds a damage response element at the reaper locus. Cell. 101:2000;103-113. Results reported in this paper indicate that the proapoptotic reaper gene is a direct transcriptional target of Drosophila p53 following DNA damage. This defines a mechanism by which p53 induces apoptosis in Drosophila cells.
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(2000)
Cell
, vol.101
, pp. 103-113
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Brodsky, M.H.1
Nordstrom, W.2
Tsang, G.3
Kwan, E.4
Rubin, G.M.5
Abrams, J.M.6
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15
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12944269061
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Identification and characterization of a p53 homologue in Drosophila melanogaster
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Jin S., Martinek S., Joo W.S., Wortman J.R., Mirkovic N., Sali A., Yandell M.D., Pavletich N.P., Young M.W., Levine A.J. Identification and characterization of a p53 homologue in Drosophila melanogaster. Proc Natl Acad Sci USA. 97:2000;7301-7306.
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(2000)
Proc Natl Acad Sci USA
, vol.97
, pp. 7301-7306
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Jin, S.1
Martinek, S.2
Joo, W.S.3
Wortman, J.R.4
Mirkovic, N.5
Sali, A.6
Yandell, M.D.7
Pavletich, N.P.8
Young, M.W.9
Levine, A.J.10
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16
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0034674713
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Gene targeting by homologous recombination in Drosophila
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This paper reports the first successful gene targeting in Drosophila. Until recently, it was impossible to inactivate Drosophila genes by homologous recombination. This paper describes a 'knock in' of the wild-type version of the yellow gene into the mutant yellow locus by homologous recombination. As the yellow locus is close to the telomere, Engels 17 raises the possibility that this apparent homologous recombination resulted from break-induced replication.
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Rong Y.S., Golic K.G. Gene targeting by homologous recombination in Drosophila. Science. 288:2000;2013-2018. This paper reports the first successful gene targeting in Drosophila. Until recently, it was impossible to inactivate Drosophila genes by homologous recombination. This paper describes a 'knock in' of the wild-type version of the yellow gene into the mutant yellow locus by homologous recombination. As the yellow locus is close to the telomere, Engels 17 raises the possibility that this apparent homologous recombination resulted from break-induced replication.
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(2000)
Science
, vol.288
, pp. 2013-2018
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Rong, Y.S.1
Golic, K.G.2
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17
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0034674604
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Reversal of fortune for Drosophila geneticists?
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Engels W.R. Reversal of fortune for Drosophila geneticists? Science. 288:2000;1973-1975.
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(2000)
Science
, vol.288
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Engels, W.R.1
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18
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0035105893
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A targeted gene knockout in Drosophila
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A description of a targeted knockout of the pugilist gene. As pugilist maps far from the telomere, the targeted recombination is unlikely to result from break-induced replication. Thus this method of gene disruption is likely to be applicable to most, if not all, Drosophila genes.
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Rong Y.S., Golic K.G. A targeted gene knockout in Drosophila. Genetics. 157:2001;1307-1312. A description of a targeted knockout of the pugilist gene. As pugilist maps far from the telomere, the targeted recombination is unlikely to result from break-induced replication. Thus this method of gene disruption is likely to be applicable to most, if not all, Drosophila genes.
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(2001)
Genetics
, vol.157
, pp. 1307-1312
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Rong, Y.S.1
Golic, K.G.2
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19
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0029024475
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Par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed
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Guo S., Kemphues K.J. par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. Cell. 81:1995;611-620.
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Cell
, vol.81
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Guo, S.1
Kemphues, K.J.2
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20
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0032545933
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Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
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Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 391:1998;806-811.
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Nature
, vol.391
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Fire, A.1
Xu, S.2
Montgomery, M.K.3
Kostas, S.A.4
Driver, S.E.5
Mello, C.C.6
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21
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0032443021
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Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the Wingless pathway
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Kennerdell J.R., Carthew R.W. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the Wingless pathway. Cell. 95:1998;1017-1026.
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Cell
, vol.95
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Kennerdell, J.R.1
Carthew, R.W.2
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22
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0034673638
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An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells
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The authors show that loss-of-function phenotypes can be created by transfecting Drosophila cells with specific double-stranded RNAs. Cell Extracts contain a nuclease with an essential RNA component that specifically degrades cognate cellular RNAs.
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Hammond S.M., Bernstein E., Beach D., Hannon G.J. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature. 404:2000;293-296. The authors show that loss-of-function phenotypes can be created by transfecting Drosophila cells with specific double-stranded RNAs. Cell Extracts contain a nuclease with an essential RNA component that specifically degrades cognate cellular RNAs.
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(2000)
Nature
, vol.404
, pp. 293-296
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Hammond, S.M.1
Bernstein, E.2
Beach, D.3
Hannon, G.J.4
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23
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0034612276
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Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways
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Clemens J.C., Worby C.A., Simonson-Leff N., Muda M., Maehama T., Hemmings B.A., Dixon J.E. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc Natl Acad Sci USA. 97:2000;6499-6503.
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Proc Natl Acad Sci USA
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Clemens, J.C.1
Worby, C.A.2
Simonson-Leff, N.3
Muda, M.4
Maehama, T.5
Hemmings, B.A.6
Dixon, J.E.7
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24
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0033883114
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Heritable gene silencing in Drosophila using double-stranded RNA
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See annotation 25•
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Kennerdell J.R., Carthew R.W. Heritable gene silencing in Drosophila using double-stranded RNA. Nat Biotechnol. 18:2000;896-898. See annotation 25•
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(2000)
Nat Biotechnol
, vol.18
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Kennerdell, J.R.1
Carthew, R.W.2
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25
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0034710618
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Inducible expression of double-stranded RNA directs specific genetic interference in Drosophila
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Expression of transgenes encoding hairpin RNA structures allows heritable gene silencing and can be used to assess the functions of genes with late developmental roles.
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Lam G., Thummel C.S. Inducible expression of double-stranded RNA directs specific genetic interference in Drosophila. Curr Biol. 24:2000;957-963. Expression of transgenes encoding hairpin RNA structures allows heritable gene silencing and can be used to assess the functions of genes with late developmental roles.
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(2000)
Curr Biol
, vol.24
, pp. 957-963
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Lam, G.1
Thummel, C.S.2
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26
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0034532329
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Specific genetic interference with behavioral rhythms in Drosophila by expression of inverted repeats
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This paper describes tissue-specific genetic interference by expressing inverted-repeat RNAs using the GAL4/UAS binary transcription system. Expression of RNA hairpin loops representing the period gene interfered with circadian clock functioning.
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Martinek S., Young M.W. Specific genetic interference with behavioral rhythms in Drosophila by expression of inverted repeats. Genetics. 156:2000;1717-1725. This paper describes tissue-specific genetic interference by expressing inverted-repeat RNAs using the GAL4/UAS binary transcription system. Expression of RNA hairpin loops representing the period gene interfered with circadian clock functioning.
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(2000)
Genetics
, vol.156
, pp. 1717-1725
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Martinek, S.1
Young, M.W.2
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28
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0028896185
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An inverse PCR screen for the detection of P element insertions in cloned genomic intervals in Drosophila melanogaster
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Dalby B., Pereira A.J., Goldstein L.S. An inverse PCR screen for the detection of P element insertions in cloned genomic intervals in Drosophila melanogaster. Genetics. 139:1995;757-766.
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Genetics
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Dalby, B.1
Pereira, A.J.2
Goldstein, L.S.3
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29
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0033760528
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Targeted recovery of mutations in Drosophila
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The authors describe the isolation of novel abnormal wing discs mutants by random EMS mutagenesis followed by mutation detection by denaturing high-performance liquid chromatography.
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Bentley A., MacLennan B., Calvo J., Dearolf C.R. Targeted recovery of mutations in Drosophila. Genetics. 156:2000;1169-1173. The authors describe the isolation of novel abnormal wing discs mutants by random EMS mutagenesis followed by mutation detection by denaturing high-performance liquid chromatography.
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(2000)
Genetics
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Bentley, A.1
MacLennan, B.2
Calvo, J.3
Dearolf, C.R.4
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30
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0026418313
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Targeted gene replacement in Drosophila via P element-induced gap repair
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Gloor G.B., Nassif N.A., Johnson-Schlitz D.M., Preston C.R., Engels W.R. Targeted gene replacement in Drosophila via P element-induced gap repair. Science. 253:1991;1110-1117.
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Gloor, G.B.1
Nassif, N.A.2
Johnson-Schlitz, D.M.3
Preston, C.R.4
Engels, W.R.5
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31
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0027488367
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Preferential transposition of Drosophila P elements to nearby chromosomal sites
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Tower J., Karpen G.H., Craig N., Spradling A.C. Preferential transposition of Drosophila P elements to nearby chromosomal sites. Genetics. 133:1993;347-359.
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Genetics
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Tower, J.1
Karpen, G.H.2
Craig, N.3
Spradling, A.C.4
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32
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0023653271
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Phenocopies induced with antisense RNA identify the wingless gene
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Cabrera C.V., Alonso M.C., Johnston P., Phillips R.G., Lawrence P.A. Phenocopies induced with antisense RNA identify the wingless gene. Cell. 50:1987;659-663.
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Cell
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Cabrera, C.V.1
Alonso, M.C.2
Johnston, P.3
Phillips, R.G.4
Lawrence, P.A.5
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33
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0027375250
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Generating loss-of-function phenotypes of the fushi tarazu gene with a targeted ribozyme in Drosophila
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Zhao J.J., Pick L. Generating loss-of-function phenotypes of the fushi tarazu gene with a targeted ribozyme in Drosophila. Nature. 365:1993;448-451.
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Nature
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Zhao, J.J.1
Pick, L.2
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34
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0032883802
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The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes
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Spradling A.C., Stern D., Beaton A., Rhem E.J., Laverty T., Mozden N., Misra S., Rubin G.M. The Berkeley Drosophila Genome Project gene disruption project: single P-element insertions mutating 25% of vital Drosophila genes. Genetics. 153:1999;135-177.
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Genetics
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Spradling, A.C.1
Stern, D.2
Beaton, A.3
Rhem, E.J.4
Laverty, T.5
Mozden, N.6
Misra, S.7
Rubin, G.M.8
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35
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0034329159
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Molecular genetics: Unmasking polyglutamine triggers in neurodegenerative disease
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Gusella J.F., MacDonald M.E. Molecular genetics: unmasking polyglutamine triggers in neurodegenerative disease. Nat Rev Neurosci. 1:2000;109-115.
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Nat Rev Neurosci
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Gusella, J.F.1
MacDonald, M.E.2
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36
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18544392423
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Expanded polyglutamine protein forms nuclear inclusions and causes neural degeneration in Drosophila
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See annotation 37•.
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Warrick J.M., Paulson H.L., Gray-Board G.L., Bui Q.T., Fischbeck K.H., Pittman R.N., Bonini N.M. Expanded polyglutamine protein forms nuclear inclusions and causes neural degeneration in Drosophila. Cell. 93:1998;939-949. See annotation 37•.
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(1998)
Cell
, vol.93
, pp. 939-949
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Warrick, J.M.1
Paulson, H.L.2
Gray-Board, G.L.3
Bui, Q.T.4
Fischbeck, K.H.5
Pittman, R.N.6
Bonini, N.M.7
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37
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0032168160
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Polyglutamine-expanded human huntingtin transgenes induce degeneration of Drosophila photoreceptor neurons
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