High-yield synthesis and purification of an α-helical transmembrane domain

Analytical Biochemistry

Volumn 293, Issue 1, 2001, Pages 102-108

  Fisher, Lillian E ^a   Engelman, Donald M ^b  

^a Department of Chemistry ^*  (United States)

^b YALE UNIVERSITY   (United States)

Author keywords

Fmoc; Hydrophobic peptides; Reverse phase HPLC; Solid phase peptide synthesis; SPPS; helix

Indexed keywords

ACTIVATION ANALYSIS; HYDROPHOBICITY; PEPTIDES; PURIFICATION;

FMOC; HYDROPHOBIC PEPTIDES; REVERSE PHASE HPLC; SOLID PHASE PEPTIDE SYNTHESIS; SPPS;

SYNTHESIS (CHEMICAL);

ARTICLE; HYDROPHOBICITY; MASS SPECTROMETRY; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 0035369984     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5122     Document Type: Article

References (16)

1. How many membrane proteins are there?
2. Genome-wide analysis of integral membrane proteins from eubacterial, archean, and eukaryotic organisms
3. Glycophorin A dimerization is driven by specific interactions between transmembrane α-helices
4. Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids
5. Standard Fmoc protocols
6. Coupling reagents and activation
7. Progress in the development of solvent and chromatography systems appropriate for bitopic membrane proteins
8. Synthetic peptides mimic the assembly of transmembrane glycoproteins
9. Preparation of insoluble transmembrane peptides: Glycophorin-A, prion (110-137), and FGFR (368-397)
10. Secondary structure, orientation, oligomerization, and lipid interactions of the transmembrane domain of influenza hemagglutinin
11. Detergents modulate dimerization, but not helicity, of the glycophorin A transmembrane domain
12. Trifluoroacetic acid cleavage and deprotection of resin-bound peptides following synthesis by Fmoc chemistry
13. Simple procedure for reversed-phase high performance liquid chromatographic purification of long hydrophobic peptides that form transmembrane helices
14. Refolding of an integral membrane protein