An electrotransfection protocol for yeast two-hybrid library screening

Analytical Biochemistry

Volumn 293, Issue 1, 2001, Pages 149-152

  Helmuth, Martin ^a   Altrock, Wilko ^a   Böckers, Tobias M ^b   Gundelfinger, Eckart D ^a   Kreutz, Michael R ^a  

^a LEIBNIZ INSTITUTE FOR NEUROBIOLOGY   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

YEAST TWO HYBRID;

CHEMISTRY;

COMPLEMENTARY DNA;

ARTICLE; DNA TRANSFECTION; ELECTROPORATION; NONHUMAN; PRIORITY JOURNAL; TWO HYBRID SYSTEM; YEAST;

EID: 0035368580     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5107     Document Type: Article

References (9)

1. High efficiency transformation of yeast by electroporation
2. A ten minute protocol for transforming Saccharomyces cerevisiae by electroporation
3. Transfer of episomal and integrated plasmids from Saccharomyces cerevisiae to Escherichia coli by electroporation
4. A novel genetic system to detect protein-protein interactions
5. High efficiency transformation of Saccharomyces cerevisiae by electroporation
6. Direct transfer of plasmid DNA from yeast to E. coli by electroporation
7. Direct plasmid shuttling from yeast into Escherichia coli by electroduction
8. Calcium-mediated DNA adsorption to yeast cells and kinetics of cell transformation by electroporation
9. An improved protocol for the preparation of yeast cells for transformation by electroporation