Characterization of a differentially expressed protein that shows an unusual localization to intracellular membranes in Leishmania major

Biochemical Journal

Volumn 356, Issue 2, 2001, Pages 335-344

  Knuepfer, E ^a   Stierhof, Y D ^a   McKean, P G ^a   Smith, D F ^a  

^a WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

Author keywords

Differential gene expression; Endoplasmic reticulum; Metacyclic; Mitochondrial protein

Indexed keywords

BIOASSAY; BIOLOGICAL MEMBRANES; MORPHOLOGY; PROTEINS;

CELL FRACTIONATION;

GENES;

PROTEIN; SMALL HYDROPHILIC ENDOPLASMIC RETICULUM ASSOCIATED PROTEIN; UNCLASSIFIED DRUG;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL FRACTIONATION; CONTROLLED STUDY; CROSS LINKING; CYTOSKELETON; ENDOPLASMIC RETICULUM; INTRACELLULAR MEMBRANE; LEISHMANIA MAJOR; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA PRIMERS; GENE EXPRESSION; GENES, PROTOZOAN; HUMANS; INTRACELLULAR MEMBRANES; ISOELECTRIC POINT; LEISHMANIA MAJOR; MICROSCOPY, IMMUNOELECTRON; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PROTEIN STRUCTURE, SECONDARY; PROTOZOAN PROTEINS;

ANIMALIA; LEISHMANIA MAJOR; MAMMALIA; PROTOZOA;

EID: 0035367421     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3560335     Document Type: Article

References (58)

1. Growth cycle-dependent generation of complement-resistant Leishmania promastigotes
2. Identification of cell surface carbohydrate and antigenic changes between noninfective and intective developmental stages of Leishmania major promastigotes
3. Identification of an infective stage of Leishmania promastigotes
4. Biochemical characteristics of the metacyclic forms of Leishmania major and L. mexicana mexicana
5. Developmental modification of lipophosphoglycan during the differentiation of Leishmania major promastigotes to an infectious stage
6. Three distinct RNAs for the surface protease gp63 are differentially expressed during development of Leishmania donovani chagasi promastigotes to an infectious form
7. The multiple cpb cysteine proteinase genes of Leishmania mexicana encode isoenzymes that differ in their stage regulation and substrate preference
8. Isolation of genes showing increased or unique expression in the infective promastigotes of Leishmania major
9. Characterisation and expression of a stage regulated gene of Leishmania major
10. Leishmania: Overexpression and comparative structural analysis of the stage-regulated meta I gene
11. Genomic organisation and expression of a differentially regulated gene family from Leishmania major
12. Characterisation of a second protein encoded by the differentially regulated Lm cDNA16 gene family of Leishmania major
13. A microtubule-associated protein in Drosophila melanogaster: Identification, characterisation and isolation of the coding sequence
14. A shuttle vector which faciliates the expression of transfected genes in Trypanosoma cruzi and Leishmania
15. Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes Proc
16. Tricine-sodium dodecylsulfate polyacrylamide gel electrophoresis for the separation of proteins in the range of 1-100 kDa
17. An indirect immuno-fluorescence procedure for staining the same cryosection with two mouse monoclonal primary antibodies
18. Molecular cloning and cellular localisation of a BiP homologue in Trypanosoma brucei
19. Use of poly(vinylpyrrolidone) and poly(vinyl alcohol) for cryoultramicrotomy
20. Preparation, use and enlargement of ultra-small gold particles in immunoelectron microscopy
21. Analysis of post-transcriptional regulation operating on transcription products of the tandemly-linked Leishmania infantum hsp70 genes
22. Differential expression of Leishmania major beta-tubulin genes during the acquisition of promastigote infectivity
23. Differential regulation of multiple glucose transporter genes in Leishmania mexicana
24. Histone synthesis in Leishmania infantum is tightly linked to DNA replication by a translational control
25. Leishmania major: Differential regulation of the surface metalloproteinase in amastigote and promastigote stages
26. Isolation and structural characterisation of the Leishmania donovani kinetoplastid membrane protein-11, a major immunoreactive membrane glycoprotein
27. Identification of a protein associated with p21ras by chemical crosslinking
28. Expression of a mitochondrial stress-related protein in the protozoan parasite Leishmania major
29. Mitochondrial hsp70/MIM44 complex facilitates protein import
30. Thymosin β4 and Fx, an actin-sequestering peptide, are indistinguishable
31. Toxofilin, a novel actin-binding protein from Toxoplasma gondii, sequesters actin monomers and caps actin filaments
32. Atomic structure of the actin: DNasel complex
33. Purification of overexpressed hexahistidine-tagged BML N431 as oligomeric complexes
34. Genes selectively expressed in the infectious (metacyclic) stage of Leishmania major promastigotes encode a potential basic-zipper structural motif
35. The gene encoding the metacyclogenesis-associated transcript Mat-1 is conserved in the genus Leishmania and shows a tendency to form dimers upon protein expression
36. Bifunctional messenger RNAs in eukaryotes
37. Using 3′ untranslated sequences to identify differentially-expressed genes in Leishmania
38. Glucose transport in amastigotes and promastigotes of Leishmania mexicana mexicana
39. Yeast acetyl-CoA carboxylase is associated with the cytoplasmic surface of the endoplasmic reticulum
40. The specific subcellular localisation of two isoforms of cytochrome b5 suggests novel targeting pathways
41. A role for N-myristoylation in protein targeting: NADH-cytochrome b5 reductase requires myristic acid for association with outer mitochondrial but not ER membranes
42. Assembly of Bcl-2 into microsomal and outer mitochondrial membranes
43. 2+ responses
44. Association between the endoplasmic reticulum and mitochondria of yeast facilitates interorganelle transport of phospholipids through membrane contact
45. Tom 5 functionally links mitochondrial preprotein receptors to the general import pore
46. Molecular architecture of the ER translocase probed by chemical crosslinking of Sss1p to complementary fragments of Sec61p
47. Species-specificity in endoplasmic reticulum signal peptide utilization revealed by proteins from Trypanosoma bruce and Leishmania
48. In vitro import of proteins into mitochondria of Trypanosoma bruce/and Leishmania tarentolae
49. A nuclear encoded tRNA of Trypanosoma brucei is imported into mitochondria
50. Association of mitochondria with microtubules in cultured cells
51. Actin structure and function: Roles in mitochondrial organization and morphogenesis in budding yeast and identification of the phalloidin-binding site