Comparison of Polymerase Chain Reaction on Fresh Tissue Samples and Fecal Drops on Filter Paper for Detection of Trypanosoma cruzi in Rhodnius prolixus

Memorias do Instituto Oswaldo Cruz

Volumn 96, Issue 4, 2001, Pages 503-505

  Dorn, P L ^a   Flores, J ^a   Brahney, B ^a   Gutierrez, A ^a   Rosales, R ^b   Rodas, A ^b   Monroy, C ^b  

^a LOYOLA UNIVERSITY   (United States)

^b NONE

Author keywords

PCR; Rhodnius prolixus; Trypanosoma cruzi

Indexed keywords

AGAR GEL ELECTROPHORESIS; ANIMAL; ARTICLE; COMPARATIVE STUDY; DISEASE CARRIER; FECES; FILTRATION; INTESTINE; ISOLATION AND PURIFICATION; METHODOLOGY; PAPER; PARASITOLOGY; POLYMERASE CHAIN REACTION; RHODNIUS; TRYPANOSOMA CRUZI;

ANIMALS; ELECTROPHORESIS, AGAR GEL; FECES; FILTRATION; INSECT VECTORS; INTESTINES; PAPER; POLYMERASE CHAIN REACTION; RHODNIUS; TRYPANOSOMA CRUZI;

EID: 0035350718     PISSN: 00740276     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0074-02762001000400010     Document Type: Article

References (5)

1. Brenière SF, Bosseno MF, Telleria J, Carrasco R, Vargas F, Yaksic N, Noireau F 1995. Field application of polymerase chain reaction diagnosis and strain typing of Trypanosoma cruzi in Bolivian triatomines. Am J Trop Med Hyg 53: 179-184.
2. Britto C, Cardoso MA, Wincker P, Morel CM 1993. A simple protocol for the physical cleavage of Trypanosoma cruzi kinetoplast DNA present in blood samples and its use in polymerase chain reaction (PCR)-based diagnosis of chronic Chagas disease. Mem Inst Oswaldo Cruz 88: 171-172.
3. D'Alessandro-Bacigalupo A, Saravia N G 1992. Trypanosoma rangeli. In J Kreier, J Baker (eds), Parasitic Protozoa, Academic Press, San Diego, p. 1-55.
4. Dorn P, Engelke D, Rodas A, Rosales R, Melgar S, Brahney B, Flores J, Monroy C 1999. Utility of the polymerase chain reaction in detection of Trypanosoma cruzi in Guatemalan Chagas' disease vectors. Am J Trop Med Hyg 60: 740-745.
5. Dorn PL, Selgean S, Guillot M 1997. Simplified method for preservation and polymerase chain reaction-amplification of Trypanosoma cruzi DNA in human blood. Mem Inst Oswaldo Cruz 92: 253-255.