Zymography with caseogram prints: Quantification of pepsinogen

Analytical Biochemistry

Volumn 292, Issue 1, 2001, Pages 22-25

  Till, Olaf ^a   Baumann, Eckehard ^a   Linss, Werner ^a  

^a JENA UNIVERSITY HOSPITAL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

ACID PROTEASE; AGAROSE; DETECTION LIMITS; PROTEASE ACTIVITIES; SKIM MILKS; ZYMOGRAPHY;

ENZYME ACTIVITY;

ACID PROTEINASE; AGAROSE; PEPSINOGEN; PROTEINASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME DEGRADATION; INTERMETHOD COMPARISON; MILK; PRIORITY JOURNAL; REPRODUCIBILITY; ZYMOGRAPHY;

EID: 0035337808     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5055     Document Type: Article

References (13)

1. Quantitative zymography: Detection of picogram quantities of gelatinases
2. Zymography: A singlestep staining method for quantitation of proteolytic activity on substrate gels
3. Detection of proteases by clotting of casein after gel electrophoresis
4. Electrophoretic analysis of a gastric cancer-associated acid proteinase using a highly sensitive detection system
5. Conversion of cathepsin E to enzymatic unstable form in gastric cancer cells
6. Purification of pepsinogens from human urine and electrophoretic analysis by caseogram print
7. Activation of porcine pepsinogen A. The stability of two non-covalent activation inter-mediates at pH 8.5
8. Occurrence of two different pathways in the activation of porcine pepsinogen to pepsin
9. The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin
10. Modification of a discontinuous and highly porous sodium dodecyl sulfate-polyacrylamide gel system for minigel electrophoresis
11. Collagen zymography as a sensitive and specific technique for the determination of subpicogram levels of interstitial collagenase
12. Isolation, crystallization, and properties of swine pepsinogen
13. The origin of the alkaline inactivation of pepsinogen