Molecular basis for Rac2 regulation of phagocyte NADPH oxidase

Nature Immunology

Volumn 2, Issue 3, 2001, Pages 211-215

  Diebold, Becky A ^a   Bokoch, Gary M ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME B; FLAVINE ADENINE NUCLEOTIDE; NEUTROPHIL CYTOSOL FACTOR 67K; NEUTROPHIL CYTOSOLIC FACTOR 1; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PHOSPHOPROTEIN; RAC PROTEIN; RAC2 PROTEIN; REACTIVE OXYGEN METABOLITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE;

ANIMAL; ARTICLE; CHEMISTRY; ELECTRON TRANSPORT; ENZYMOLOGY; GENETICS; IMMUNOLOGY; METABOLISM; PHAGOCYTE; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

ANIMALS; CYTOCHROME B GROUP; ELECTRON TRANSPORT; FLAVIN-ADENINE DINUCLEOTIDE; MUTAGENESIS, SITE-DIRECTED; NADP; NADPH OXIDASE; PHAGOCYTES; PHOSPHOPROTEINS; PROTEIN STRUCTURE, TERTIARY; RAC GTP-BINDING PROTEINS; REACTIVE OXYGEN SPECIES;

EID: 0035293023     PISSN: 15292908     EISSN: None     Source Type: Journal    
DOI: 10.1038/85259     Document Type: Article

References (35)

1. Finkel, T. Signal transduction by reactive oxygen species in non-phagocytic cells. J. Leukoc. Biol. 65, 337-340 (1999).
2. Babior, B. M. NADPH oxidase: an update. Blood 93, 1464-1476 (1999).
3. Suh, Y. A. et al. Cell transformation by the superoxide-generating oxidase Mox1. Nature 401, 79-82 (1999).
4. + channel generated through alternative splicing of the NADPH oxidase homolog NOH-1. Science 287, 138-142 (2000).
5. De Deken, X. et al. Cloning of two human thyroid cDNAs encoding new members of the NADPH oxidase family. J. Biol. Chem. 275, 23227-23233 (2000).
6. Bokoch, G. M. Regulation of the human neutrophil NADPH oxidase by the Rac GTP-binding proteins. Curr. Opin. Cell Biol. 6, 212-218 (1994).
7. Dorseuil, O. et al. Inhibition of superoxide production in B lymphocytes by Rac antisense oligonucleotides. J. Biol. Chem. 267, 20540-20542 (1992).
8. Roberts, A. W. et al. Deficiency of the hematopoietic cell-specific Rho family GTPase Rac2 is characterized by abnormalities in neutrophil function and host defense. Immunity 10, 183-196 (1999).
9. Ambruso, D. et al. Human neutrophil immunodeficiency syndrome is associated with an inhibitory Rac2 mutation. Proc. Natl Acad. Sci. USA 97, 4654-4659 (2000).
10. Voncken, J. W. et al. Increased neutrophil respiratory burst in Bcr null mutants. Cell 80, 719-728 (1995).
11. Quinn, M. T., Evans, T., Loetterle, L. R., Jesaitis, A. J. & Bokoch, G. M. Translocation of Rac correlates with NADPH oxidase activation: Evidence for equimolar translocation of oxidase components. J. Biol. Chem. 268, 20983-20987 (1993).
12. Philips, M. R., Feoktistov, A., Pillinger, M. H. & Abramson, S. B. Translocation of p21rac2 from cytosol to plasma membrane is neither necessary nor sufficient for neutrophil NADPH oxidase activity. J. Biol. Chem. 270, 11514-11521 (1995).
13. Dorseuil, O., Quinn, M. T. & Bokoch, G. M. Dissociation of Rac translocation from p47phox/p67phox movements in human neutrophils by tyrosine kinase inhibitors. J. Leukoc. Biol. 58, 108-113 (1995).
14. Heyworth, P. G. et al. Requirement for post-translational processing of Rac GTP-binding proteins for activation of human neutrophil NADPH oxidase. Mol. Biol. Cell 4, 261-269 (1993).
15. -254. J. Biol. Chem. 267, 16767-16770 (1992).
16. Cross, A. R., Yarchover, J. L. & Curnutte, J. T. The superoxide-generating system of human neutrophils possesses a novel diaphorase activity: Evidence for distinct regulation of electron flow within NADPH oxidase by p67phox and p47phox. J. Biol. Chem. 269, 21448-21454 (1994).
17. Cross, A., Heyworth, P., Rae, J. & Curnutte, J. A variant x-linked chronic granulomatous disease patient (X91+) with partially functional cytochrome b. J. Biol. Chem. 270, 8194-5200 (1995).
18. 245 of the neutrophil superoxide-generating sytem contains two non-identical hemes. J. Biol. Chem. 270, 17075-17077 (1995).
19. Bromberg, Y. & Pick, E. Activation of NADPH-dependent superoxide production in a cell-free system by sodium dodecyl sulfate. J. Biol. Chem. 260, 13539-13545 (1985).
20. Cross, A. R. & Curnutte, J. T. The cytosolic factors p47-phox and p67-phox have distinct roles in the regulation of electron flow in NADPH oxidase. J. Biol. Chem. 270, 6543-6548 (1995).
21. Freeman, J. L. & Lambeth, J. D. NADPH oxidase activity is independent of p47-phox in vitro. J. Biol. Chem. 271, 22578-22582 (1996).
22. Koshkin, V., Lotan, O. & Pick, E. The cytosolic component p47(phox) is not a sine qua non participant in the activation of NADPH oxidase but is required for optimal superoxide production. J. Biol. Chem. 271, 30326-30329 (1996).
23. Diekmann, D., Abo, A., Johnston, C., Segal, A. W. & Hall, A. Interaction of Rac with p67phox and regulation of phagocytic NADPH oxidase activity. Science 265, 531-533 (1994).
24. Xu, X., Barry, D. C., Settleman, J., Schwartz, M. A. & Bokoch, G. M. Differing structural requirements for GTPase-activating protein responsiveness and NADPH oxidase activation by Rac. J. Biol. Chem. 269, 23569-23574 (1994).
25. Nisimoto, Y., Freeman, J. L. R., Motalebi, S. A., Hirshberg, M. & Lambeth, J. D. Rac binding to p67phox: Structural basis for interactions of the Rac1 effector region and insert region with components of the respiratory burst oxidase. J. Biol. Chem. 272, 18834-18841 (1997).
26. 558. J. Biol. Chem. 269, 30749-30752 (1994).
27. Freeman, J. L., Abo, A. & Lambeth, J. D. Rac "insert region" is a novel effector region that is implicated in the activation of NADPH oxidase, but not PAK65. J. Biol. Chem. 271, 19794-19801 (1996).
28. Joneson, T. & Bar-Sagi, D. A Rac1 effector site controlling mitogenesis through superoxide production. J. Biol. Chem. 273, 17991-17994 (1998).
29. Hiratsuka, T. New ribose-modified fluorescent analogs of adenine and guanine nucleotides available as substrates for various enzymes. Biochim. Biophys. Acta 742, 496-508 (1983).
30. Han, C. H., Freeman, J. L., Lee, T., Motalebi, S. A. & Lambeth, J. D. Regulation of the neutrophil respiratory burst oxidase. Identification of an activation domain in p67(phox). J. Biol. Chem. 273, 16663-16668 (1998).
31. 558. J. Biol. Chem. 274, 22999-23005 (1999).
32. -245 are required for the activation of NADPH oxidase by anionic amphiphiles. J. Biol. Chem. 274, 15519-15525 (1999).
33. Guan, K. & Dixon, J. Eucaryotic protein expression in E. coli: An improved thrombin cleavage and purification procedure effusion proteins with glutathione S-transferase. Anal. Biochem. 192, 262-267 (1991).
34. phox. J. Biol. Chem. 273, 15693-15701 (1998).
35. -245 is a flavocytochrome containing FAD and the NADPH-binding site of the microbicidal oxidase of phagocytes. Biochem. J. 284, 781-788 (1992).