Biological Sensor for Sucrose Availability: Relative Sensitivities of Various Reporter Genes

Applied and Environmental Microbiology

Volumn 67, Issue 3, 2001, Pages 1308-1317

  Miller, William G ^a,b   Brandl, Maria T ^a,b   Quiñones, Beatriz ^a   Lindow, Steven E ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b WESTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOTOPROTEIN; PORIN; REPRESSOR PROTEIN; SCRR PROTEIN, BACTERIA; SCRY PROTEIN, BACTERIA; SUCROSE;

ARTICLE; ERWINIA; GENE EXPRESSION REGULATION; GENETIC PROCEDURES; GENETIC TRANSCRIPTION; GENETICS; LACTOSE OPERON; LEGUME; MEDICINAL PLANT; METABOLISM; MICROBIOLOGY; PHYSIOLOGY; PLASMID; PROMOTER REGION; REPORTER GENE; SALMONELLA TYPHIMURIUM;

BACTERIAL PROTEINS; BIOSENSING TECHNIQUES; ERWINIA; FABACEAE; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; LAC OPERON; LUMINESCENT PROTEINS; PLANTS, MEDICINAL; PLASMIDS; PORINS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; SALMONELLA TYPHIMURIUM; SUCROSE; TRANSCRIPTION, GENETIC;

EID: 0035291227     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.3.1308-1317.2001     Document Type: Article

References (40)

1. Amann, R. I. 1995. In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes, p. 3.3.6/1-3.3.6/15. In A. D. L. Akkermans, J. D. Van Elsas, and F. J. de Bruijn (ed.), Molecular microbial ecology manual. Kluwer Academic Publishers, Dordrecht, The Netherlands.
2. Barker, L. P., D. M. Brooks, and P. L. Small. 1998. The identification of Mycobacterium marinum genes differentially expressed in macrophage phagosomes using promoter fusions to green fluorescent protein. Mol. Microbiol. 29:1167-1177.
3. Bolivar, F., R. L. Rodriguez, P. J. Greene, M. C. Betlach, H. L. Heyneker, and H. W. Boyer. 1977. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95-113.
4. Brandi, T. M., and S. E. Lindow. 1998. Contribution of indole-3-acetic acid production to the epiphytic fitness of Erwinia herbicola. Appl. Environ. Microbiol. 64:3256-3263.
5. Burlage, R. S., and C. T. Kuo. 1994. Living biosensors for the management and manipulation of microbial consortia. Annu. Rev. Microbiol. 48:291-309.
6. Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science 263:802-805.
7. Cowan, P. J., H. Nagesha, L. Leonard, J. L. Howard, and A. J. Pittard. 1991. Characterization of the major promoter for the plasmid-encoded sucrose genes scrY, scrA, and scrB. J. Bacteriol. 173:7464-7470.
8. De Weger, L. A., L. C. Dekkers, A. J. Van Der Bij, and B. J. J. Lugtenberg. 1994. Use of phosphate-reporter bacteria to study phosphate limitation in the rhizosphere and in bulk soil. Mol. Plant-Microbe Interact. 7:32-38.
9. Dumas, B., S. Centis, N. Sarrazin, and M. T. Esquerre-Tugaye. 1999. Use of green fluorescent protein to detect expression of an endopolygalacturonase gene of Colletotrichum lindemuthianum during bean infection. Appl. Environ. Microbiol. 65:1769-1771.
10. Duncan, S., L. A. Glover, K. Killham, and J. I. Prosser. 1994. Luminescence-based detection of activity of starved and viable but nonculturable bacteria. Appl. Environ. Microbiol. 60:1308-1316.
11. Green, R. L., and G. J. Warren. 1985. Physical and functional repetition in a bacterial ice nucleation gene. Nature (London) 317:645-648.
12. Heim, R., D. C. Prasher, and R. Y. Tsien. 1994. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA 91:12501-12504.
13. Hirano, S. S., E. V. Nordheim, D. C. Arny, and C. D. Upper. 1982. Lognormal distribution of epiphytic bacterial populations on leaf surfaces. Appl. Environ. Microbiol. 44:695-700.
14. Ishimaru, C., K. M. Eskridge, and A. K. Vidaver. 1991. Distribution analysis of naturally occurring epiphytic populations of Xanthomonas campestris pv. phaseoli on dry beans. Phytopathology 82:262-268.
15. Jacobi, C. A., A. Roggenkamp, A. Rakin, R. Zumbihl, L. Leitritz, and J. Heesemann. 1998. In vitro and in vivo expression studies of yopE from Yersinia enterocolitica using the gfp reporter gene. Mol. Microbiol. 30:865-882.
16. Jaeger, C. H., III, S. E. Lindow, W. Miller, E. Clark, and M. K. Firestone. 1999. Mapping of sugar and amino acid availability in soil around roots with bacterial sensors of sucrose and tryptophan. Appl. Environ. Microbiol. 65: 2685-2690.
17. Jahreis, K., and J. W. Lengeler. 1993. Molecular analysis of two ScrR repressors and of a ScrR-FruR hybrid repressor for sucrose and D-fructose specific regulons from enteric bacteria. Mol. Microbiol. 9:195-209.
18. Kay, R., and J. McPherson. 1987. Hybrid pUC vectors for addition of new restriction enzyme sites to the ends of DNA fragments. Nucleic Acids Res. 15:2778.
19. Kinkel, L., M. Wilson, and S. E. Lindow. 1995. Effects of scale on estimates of epiphytic bacterial populations. Microb. Ecol. 29:283-297.
20. Lindgren, P. B., R. Frederick, A. G. Govindarajan, N. J. Panopoulos, B. J. Staskawicz, and S. E. Lindow. 1989. An ice nucleation reporter gene system: identification of inducible pathogenicity genes in Pseudomonas syringae pv. phaseolicola. EMBO J. 8:1291-1301.
21. Lindow, S. E. 1990. Bacterial ice nucleation activity, p. 185-198. In S. Klement, K. Rudolf, and D. C. Sands (ed.), Methods in phytobacteriology. Akademiai Kiado, Budapest, Hungary.
22. Lindow, S. E. 1995. The use of reporter genes in the study of microbial ecology. Mol. Ecol. 4:555-566.
23. Loper, J. E., and M. D. Henkels. 1997. Availability of iron to Pseudomonas fluorescens in rhizosphere and bulk soil evaluated with an ice nucleation reporter gene. Appl. Environ. Microbiol. 63:99-105.
24. Loper, J. E., and S. E. Lindow. 1994. A biological sensor for iron available to bacteria in their habitats on plant surfaces. Appl. Environ. Microbiol. 60: 1934-1941.
25. Maloney, P. C., and B. Rotman. 1973. Distribution of suboptimally induced -D-galactosidase in Escherichia coli. The enzyme content of individual cells. J. Mol. Biol. 73:77-91.
26. Martinez, D., and Whitehouse, F., Jr. 1973. Selective autocytotoxicity in a model system of Escherichia coli K-12 recombinants. J. Bacteriol. 114:882-884.
27. Meikle, A., L. A. Glover, K. Killham, and J. I. Prossor. 1994. Potential luminescence as an indicator of activation of genetically-modified Pseudomonas fluorescens in liquid culture and in soil. Soil Biol. Biochem. 26:747-755.
28. Mercier, J., and S. E. Lindow. 2000. Role of leaf surface sugars in colonization of plants by bacterial epiphytes. Appl. Environ. Microbiol. 66:369-374.
29. Miller, W. G., and S. E. Lindow. 1997. An improved GFP cloning cassette designed for prokaryotic transcriptional fusions. Gene 191:144-153.
30. Miller, W. G., J. H. J. Leveau, and S. E. Lindow. 2000. Improved gfp and inaZ broad-host-range promoter-probe vectors. Mol. Plant-Microbe Interact. 13:1243-1250.
31. Mo, Y. Y., and D. C. Gross. 1991. Expression in vitro and during plant pathogenesis of the syrB gene required for syringomycin production by Pseudomonas syringae pv. syringae. Mol. Plant-Microbe Interact. 4:28-36.
32. Novick, A., and M. Weiner. 1957. Enzyme induction as an all-or-none phenomenon. Proc. Natl. Acad. Sci. USA 43:553-566.
33. Poulsen, L. K., H. M. Dalton, M. L. Angles, K. C. Marshall, S. Molin, and A. E. Goodman. 1997. Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures. Appl. Environ. Microbiol. 63:3698-3702.
34. Schmid, K., R. Ebner, J. Altenbuchner, R. Schmitt, and J. W. Lengeler. 1988. Plasmid-mediated sucrose metabolism in Escherichia coli K12: mapping of the scr genes of pUR400. Mol. Microbiol. 2:1-8.
35. Schmid, K., M. Schupfner, and R. Schmitt. 1982. Plasmid-mediated uptake and metabolism of sucrose by Escherichia coli K-12. J. Bacteriol. 151:68-76.
36. Siegele, D. A., and J. C. Hu. 1997. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc. Natl. Acad. Sci. USA 94:8168-8172.
37. Simons, R. W., F. Houman, and N. Kleckner. 1987. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53: 85-96.
38. Valdivia, R. H., and S. Falkow. 1496. Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction. Mol. Microbiol. 22:367-378.
39. Webb, C. D., A. Decatur, A. Teleman, and R. Losick. 1995. Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis. J. Bacteriol. 177:5906-5011.
40. Wilson, M., and S. E. Lindow. 1994. Coexistence among epiphytic bacterial populations mediated through nutritional resource partitioning. Appl. Environ. Microbiol. 60:4468-4477.