Ca2+-sensitive cytosolic nucleases prevent efficient delivery to the nucleus of injected plasmids

Journal of Gene Medicine

Volumn 3, Issue 2, 2001, Pages 153-164

  Pollard, Hélène ^a   Toumaniantz, Gilles ^a   Amos, Jean Luc ^a   Avet Loiseau, Hervé ^a   Guihard, Gilles ^a   Behr, Jean Paul ^b   Escande, Denis ^a  

^a HÔTEL DIEU   (France)

^b CNRS   (France)

Author keywords

Gene therapy; Non viral vectors; Nucleases; Plasmid degradation; Poly(ethyleneimine)

Indexed keywords

MAMMALIA;

CALCIUM; COMPLEMENTARY DNA; ENZYME; PRIMER DNA;

ANIMAL; ARTICLE; CELL LINE; CELL NUCLEUS; CYTOSOL; ENZYMOLOGY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE TRANSFER; HUMAN; METABOLISM; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION;

ANIMALS; BASE SEQUENCE; CALCIUM; CELL LINE; CELL NUCLEUS; CYTOSOL; DNA PRIMERS; DNA, COMPLEMENTARY; ENZYMES; GENE TRANSFER TECHNIQUES; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; PLASMIDS; POLYMERASE CHAIN REACTION;

EID: 0035287881     PISSN: 1099498X     EISSN: None     Source Type: Journal    
DOI: 10.1002/jgm.160     Document Type: Article

References (31)

1. Wrobel I, Collins D. Fusion of cationic liposomes with mammalian cells occurs after endocytosis. Biochim Biophys Acta 1995; 1235: 296-304.
2. Zhou X, Huang L. DNA transfection mediated by cationic liposomes containing lipopolysine: characterization and mechanism of action. Biochim Biophys Acta 1994; 1189: 195-203.
3. Zabner J, Fasbender AJ, Moninger T, et al. Cellular and molecular barriers to gene transfer by a cationic lipid. J Biol Chem 1995; 270: 18997-19007.
4. Coonrod A, Li FQ, Horwitz M. On the mechanism of DNA transfection: efficient gene transfer without viruses. Gene Ther 1997; 4: 1313-1321.
5. Ross GF, Bruno MD, Uyeda M, et al. Enhanced reporter gene expression in cells transfected in the presence of DMI-2, an acid nuclease inhibitor. Gene Ther 1998; 5: 1244-1250.
6. Bielinska AU, Kukowska-Latallo JF, Baker JR. The interaction of plasmid DNA with polyamidoamine dendrimers: mechanism of complex formation and analysis of alterations induced in nuclease sensitivity and transcriptional activity of the complexed DNA. Biochim Biophys Acta 1997; 1353: 180-190.
7. Xu Y, Szoka FC. Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection. Biochemistry 1996; 35: 4616-5623.
8. Lechardeur D, Sohn KJ, Haardt M, et al. Metabolic instability of plasmid DNA in the cytosol: a potential barrier to gene transfer. Gene Ther 1999; 6: 482-497.
9. Pollard H, Remy JS, Loussouarn G, et al. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells. J Biol Chem 1998; 273: 7507-7511.
10. Demolombe S, Baró I, Péréon Y, et al. A Dominant negative isoform of the long QT syndrome 1 gene product. J Biol Chem 1998; 273: 6837-6843.
11. 2+ release channels? FEBS Lett 1997; 414: 89-94.
12. Wong TK, Decker GL, Lennarz WJ. Localization of dolichol in the lysosomal fraction of rat liver. J Biol Chem 1982; 257: 6614-6618.
13. Kharbanda KK, McVicker DL, Zetterman RK, et al. Ethanol consumption alters trafficking of lysosomal enzymes and affects the processing of procathepsin L in rat liver. Biochim Biophys Acta 1996; 1291: 45-52.
14. Dowty ME, Williams P, Zhang G, et al. Plasmid DNA entry into postmitotic nuclei of primary rat myotubes. Proc Natl Acad Sci U S A 1995; 92: 4572-4576.
15. Ciolina C, Byk G, Blanche F, et al. Coupling of nuclear localization signals to plasmid DNA and specific interaction of the conjugates with Importin α. Bioconjug Chem 1999; 10: 49-55.
16. Kadonaga JT, Carner KR, Masiarz FR, et al. Isolation of cDNA encoding transcription Sp1 and functional analysis of the DNA binding domain. Cell 1987; 51: 1079-1090.
17. 2+ to discriminate endonucleases activated during apoptosis. Biochimie 1997; 79: 435-138.
18. Crook K, McLachlan G, Stevenson BJ, et al. Plasmid DNA molecules complexed with cationic liposomes are protected from degradation by nucleases and shearing by aerosolisation. Gene Ther 1996; 3: 834-839.
19. Labat-Moleur F, Steffan AM, Brisson C, et al. An electron microscopy study into the mechanism of gene transfer with lipopolyamines. Gene Ther 1996; 3: 1010-1017.
20. Escriou V, Ciolina C, Helbling-Leclerc A, et al. Cationic lipid-mediated transfer: analysis of cellular uptake and nuclear import of plasmid DNA. Cell Biol Toxicol 1998; 14: 95-104.
21. Mirzayans R, Aubin RA, Paterson MC. Differential expression and stability of foreign genes introduced into human fibroblasts by nuclear versus cytoplasmic microinjection. Mutat Res 1992; 281: 115-122.
22. Barry ME, Pinto-Gonzalez D, Orson FM, et al. Role of endogenous endonucleases and tissue site in transfection and CpG-mediated immune activation after naked DNA injection. Hum Gene Ther 1999; 10: 2461-2480.
23. Dissing J, Rangaard B, Christensen U. Activity modulation of the fast and slow isozymes of human cytosolic low-molecular-weight acid phosphatase (ACP1) by purines. Biochim Biophys Acta 1993; 1162: 275-282.
24. Terada T. Characterization of multiple acid phosphatases in bovine liver cytosol and lysosome. Inactivation of cytosolic enzymes by disulfides and its redox regulation by thioltransferase. Int J Biochem Cell Biol 1997; 29: 985-992.
25. Chen CH, Chen SC. Evidence of acid phosphatase in the cytoplasm as a distinct entity. Biochim Biophys Acta 1988; 262: 427-438.
26. Dong DL, Hart GW. Purification and characterization of an O-GlcNAc selective N-acetyl-beta-D-glucosaminidase from rat spleen cytosol. J Biol Chem 1994; 269: 19321-19330.
27. Krieser RJ, Eastman A. The cloning and expression of human Deoxyribonuclease II. J Biol Chem 1998; 273: 30909-30914.
28. Fraser MJ, Tynan SJ, Papaioannou A, et al. Endo-exonuclease of human leukaemic cells: evidence for a role in apoptosis. J Cell Sci 1996; 109: 2343-2360.
29. Lacks SA. Deoxyribonuclease I in mammalian tissues. J Biol Chem 1981; 256: 2644-2648.
30. Tanuma SI, Shiokawa D. Multiple forms of nuclear deoxyribonuclease in rat thymocytes. Biochim Biophys Res Commun 1994; 203: 789-797.
31. Couture C, Chow TY. Purification and characterization of a mammalian endo-exonuclease. Nucleic Acids Res 1992; 20: 4355-4361.