Evaluation of human sperm membrane integrity using the water test and the hypoosmotic test

Andrologia

Volumn 33, Issue 2, 2001, Pages 75-77

  Bahamondes, L ^a,b   Fazano, F ^a   De Lucio, M A ^a   Neves, P A ^a   Bottcher Luiz, F ^a   Lorenzetti, G B ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b NONE   (Brazil)

Author keywords

Human sperm; Hypoosmotic swelling test; Water test

Indexed keywords

CITRATE SODIUM; CITRIC ACID; FLUORESCENT DYE; FRUCTOSE; HYPOTONIC SOLUTION; WATER;

ARTICLE; CELL MEMBRANE; HUMAN; MALE; PHYSIOLOGY; SPERMATOZOON; ULTRASTRUCTURE;

CELL MEMBRANE; CITRATES; FLUORESCENT DYES; FRUCTOSE; HUMANS; HYPOTONIC SOLUTIONS; MALE; SPERMATOZOA; WATER;

EID: 0035287565     PISSN: 03034569     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1439-0272.2001.00374.x     Document Type: Review

References (5)

1. Fazano F, Burmeister ML, Lucio MA, Bottcher Luiz F, Neves PA, Bahamondes L (1993) Correlation between hypoosmotic swelling test and 'water test' to assess human sperm membrane integrity. Andrologia 25:351-353.
2. Fuse H, Ohta S, Sakamoto M, Kazama T, Katayama T (1993) Hypoosmotic swelling test with a medium of distilled water. Arch Androl 30:111-116.
3. Jeyendran RS, Van der Ven H, Perez-Pelaez M, Crabo B, Zaneveld LJ (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 70:219-228.
4. Lin M-H, Morshedi M, Srisombut C, Nassar A, Oehninger S (1998) Plasma membrane integrity of cryopreserved human sperm: an investigation of the results of the hypoosmotic swelling test, the water test, and eosin-Y staining. Fertil Steril 70:1148-1155.
5. Lomeo A, Giambersio A (1991) 'Water test': a simple method to assess sperm-membrane integrity. Int J Androl 14: 278-282.