Detection of Orientia tsutsugamushi (Rickettsiales: Rickettsiaceae) in unengorged chiggers (Acari: Trombiculidae) from oita prefecture, Japan, by nested polymerase chain reaction

Journal of Medical Entomology

Volumn 38, Issue 2, 2001, Pages 308-311

  Pham, X D ^a   Otsuka, Y ^a   Suzuki, H ^b   Takaoka, H ^a  

^a OITA UNIVERSITY   (Japan)

^b NAGASAKI UNIVERSITY   (Japan)

Author keywords

Chigger; Orientia tsutsugamushi; Trombiculid mite; Tsutsugamushi disease; Unengorged larvae

Indexed keywords

ANIMAL; ARTICLE; GENETICS; ISOLATION AND PURIFICATION; JAPAN; METHODOLOGY; MICROBIOLOGY; MITE; ORIENTIA TSUTSUGAMUSHI; POLYMERASE CHAIN REACTION;

ANIMALS; JAPAN; MITES; ORIENTIA TSUTSUGAMUSHI; POLYMERASE CHAIN REACTION;

EID: 0035287245     PISSN: 00222585     EISSN: None     Source Type: Journal    
DOI: 10.1603/0022-2585-38.2.308     Document Type: Article

References (26)

1. Frances, S. P., P. Watcharapichat, D. Phulsuksombati, and P. Tanskul. 2000. Transmission of Orientia tsutsugamushi, the aetiological agent for scrub typhus, to co-feeding mites. Parasitology 120: 601-607.
2. Kawamori, F., M. Akiyama, M. Sugieda, T. Kanda, S. Akahane, K. Uchikawa, Y. Yamada, N. Kumada, Y. Furuya, Y. Yoshida, S. Yamamoto, N. Ohashi, and A. Tamura. 1992. Epidemiology of tsutsugamushi disease in relation to the serotypes of Rickettsia tsutsugamushi isolated from patients, field mice, and unfed chiggers on the eastern slope of Mount Fuji, Shizuoka Prefecture, Japan. J. Clin. Microbiol. 30: 2842-2846.
3. Kawamori, F., M. Akiyama, M. Sugieda, T. Kanda, S. Akahane, S. Yamamoto, N. Ohashi, and A. Tamura. 1993. Two-step polymerase chain reaction for diagnosis of scrub typhus and identification of antigenic variants of Rickettsia tsutsugamushi. J. Vet. Med. Sci. 55: 749-755.
4. Kawamura, A. Jr., H. Tanaka, and A. Tamura. 1995. Tsutsugamushi disease. University of Tokyo Press, Tokyo, Japan.
5. Ogawa, M., S. Tsukamoto, and T. Ono. 1997. Tsutsugamushi disease in Oita Prefecture, 1992-1997. Annual report of Oita Prefecture Institute of Health and Environment, Oita Prefecture 25: 21-26.
6. Ohashi, N., A. Tamura, H. Sakurai, and S. Yamamoto. 1990a. Characterization of a new antigenic type, Kuroki, of Rickettsia tsutsugamushi isolated from a patient in Japan. J. Clin. Microbiol 28: 2111-2113.
7. Ohashi, N., H. Nashimoto, H. Ikeda, and A. Tamura. 1990b. Cloning and sequencing of the gene (tsg56) encoding a type-specific antigen from Rickettsia tsutsugamushi. Gene 91: 119-122.
8. Ohashi, N., H. Nashimoto, H. Ikeda, and A. Tamura. 1992. Diversity of Immunodominant 56-kDa Type-specific Antigen (TSA) of Rickettsia tsutsugamushi. J. Biol. Chem. 267: 12728-12735.
9. Pham, X. D., H. Suzuki, Y. Otsuka, and H. Takaoka. 1999. Trombiculid fauna and seasonal occurrence of Leptotrombidium scutellare and Leptotrombidium pallidum in endemic and non-endemic areas for tsutsugamushi disease in Oita Prefecture, Japan. J. Med. Entomol. Zool. 50: 303-312.
10. Rai, J., and D. Bandopadhyay. 1978. Vertical transmission in chigger borne rickettsiosis. Indian J. Med. Res. 68: 31-38.
11. Rapmund, G., R. W. Upham, Jr., W. D. Kundin, C. Manikumaran, and T. C. Chan. 1969. Transovarial development of scub typhus rickettsiae in a colony of vector mites. Trans. R. Soc. Trop. Med. Hyg. 63: 251-258.
12. Rapmund, G. 1984. Rickettsial disease of the Far East: new perspectives. J. Infec. Dis. 149: 330-338.
13. Stover, C. K., D. P. Marana, J. M. Carter, B. A. Roe, E. Mardis, and E. V. Oaks. 1990. The 56-Kilodalton major protein antigen of Rickettsia tsutsugamushi: molecular cloning and sequence analysis of the sta56 gene and precise identification of a strain-specific epitope. Infect. Immun. 58: 2076-2084.
14. Takahashi, M., M. Murata, S. Nogami, E. Hori, A. Kawamura, Jr., and H. Tanaka. 1988. Transovarial transmission of Rickettsia tsutsugamushi in Leptotrombidium pallidum successively reared in the laboratory. Jpn. J. Exp. Med. 58: 213-218.
15. Takahashi, M., M. Murata, E. Hori, H. Tanaka, and A. Kawamura, Jr. 1990. Transmission of Rickettsia tsutsugamushi from Apodemus speciosus, a wild rodent, to larval trombiculid mites during the feeding process. Jpn. J. Exp. Med. 60: 203-208.
16. Takahashi, M., M. Murata, H. Misumi, E. Hori, A. Kawamura, Jr., and H. Tanaka. 1994. Failed vertical transmission of Rickettsia tsutsugamushi (Rickettsiales: Rickettsiaceae) acquired from rickettsemic mice by Leptotrombidium pallidium (Acari: Trombiculidae). J. Med. Entomol. 31: 212-216.
17. Tamura, A., K. Takahashi, T. Tsuruhara, H. Urakami, S. Miyamura, H. Sekikawa, M. Kenmotsu, M. Shibata, S. Abe, and H. Nezu. 1984. Isolation of Rickettsia tsutsugamushi antigenically different from Kato, Karp and Gilliam strains from patients. Microbiol. Immunol. 28: 873-882.
18. Tamura, A., N. Ohashi, H. Urakami, K. Takahashi, and M. Oyanagi. 1985. Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting. Infect. Immunol. 48: 671-675.
19. Toyokawa, K. 1972. Transmission of Rickettsia orientalis from experimentally infected mice to vector mites. J. Med. Entomol. 9: 593.
20. Traub, R., C. L. Wisseman, Jr., M. R. Jones, and J. J. O'Keefe. 1975. The acquisition of Rickettsia tsutsugamushi by chiggers (trombiculid mites) during the feeding process. Ann. N.Y. Acad. Sci. 266: 91-114.
21. Urakami, H., M. Takahashi, A. Tamura, and E. Hori. 1988. Electron microscopic observations of the embryo Leptotrombidium (Leptotrombidium) pallidum naturally infected with Rickettsia tsutsugamushi. Microbiol. Immunol. 32: 967-972.
22. Urakami, H., M. Takahashi, E. Hori, and A. Tamura. 1994. An ultrastructural study of vertical transmission of Rickettsia tsutsugamushi during oogenesis and spermatogenesis in Leptotrombidium pallidum. Am. J. Trop. Med. Hyg. 50: 219-228.
23. Urakami, H., and A. Tamura. 1996. Symbiotic relationship between Orientia tsutsugamushi and vector mites. Jpn. J. Bacteriol. 51: 497-511.
24. Walker, J. S., C. T. Chan, C. Manikumaran, and B. L. Elisberg. 1975. Attempts to infect and demonstrate transovarial transmission of R. tsutsugamushi in three species of Leptotrombidium mites. Ann. N.Y. Acad. Sci. 266: 80-90.
25. Yamamoto, S., N. Kawabata, A. Tamura, H. Urakami, N. Ohashi, M. Murata, Y. Yoshida, and A. Kawamura, Jr. 1986. Immunological properties of Rickettsia tsutsugamushi, Kawasaki strain, isolated from a patient in Kyushu. Microbiol. Immunol. 30: 611-620.
26. Yamamoto, S., N. Kawabata, K. Ooura, M. Murata, and Y. Minamishima. 1989. Antigenic types of Rickettsia tsutsugamushi isolated from patients with tsutsugamushi fever and their distribution in Miyazaki Prefecture. J. Jpn. Assoc. Infect. Dis. 63: 109-117.