Modulation of choline acetyltransferase synthesis by okadaic acid, a phosphatase inhibitor, and KN-62, a CaM kinase inhibitor, in NS-20Y neuroblastoma

Neurochemistry International

Volumn 38, Issue 1, 2001, Pages 75-82

  Pahud, Gabrielle ^a   Bontron, Séverine ^b   Eder Colli, Lorenza ^a  

^a R Michel S   (Switzerland)

^b UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

Author keywords

Choline acetyltransferase; Cholinergic neuroblastoma; Phosphoprotein kinase; Phosphoprotein phosphatase

Indexed keywords

1 [N,O BIS(5 ISOQUINOLINESULFONYL) N METHYLTYROSYL] 4 PHENYLPIPERAZINE; CHOLINE ACETYLTRANSFERASE; MESSENGER RNA; OKADAIC ACID;

ANIMAL CELL; ARTICLE; CELL STRUCTURE; CONTROLLED STUDY; DEPHOSPHORYLATION; ENZYME ACTIVITY; ENZYME SYNTHESIS; MOUSE; NEUROBLASTOMA CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION;

1-(5-ISOQUINOLINESULFONYL)-2-METHYLPIPERAZINE; ANIMALS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CHOLINE O-ACETYLTRANSFERASE; ENZYME INDUCTION; ENZYME INHIBITORS; GENE EXPRESSION REGULATION, NEOPLASTIC; MICE; NEOPLASM PROTEINS; NEUROBLASTOMA; OKADAIC ACID; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHORYLATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; RNA, MESSENGER; RNA, NEOPLASM; TUMOR CELLS, CULTURED;

EID: 0035241610     PISSN: 01970186     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0197-0186(00)00064-4     Document Type: Article

References (20)

1. Amano T., Richelson E., Nirenberg M. Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase-acetylcholinesterase-tyrosine hydroxylase-axons-dendrites). PNAS. 69:1972;258-263.
2. Barochovsky O., Docherty M., Bradford H.F. Membrane-bound choline acetyltransferase is a cell surface marker of the differentiated NS-20Y cholinergic cell line. Neurosci. Lett. 90:1988;320-327.
3. Bruce G., Hersh L.B. The phosphorylation of choline acetyltransferase. Neurochem. Res. 14:1989;613-620.
4. Cooke L.J., Rylett R.J. Inhibitors of serine/threonine phosphatases increase membrane-bound choline acetyltransferase activity and enhance acetylcholine synthesis. Brain Res. 751:1997;232-238.
5. Fonnum F. A radiochemical method for the estimation of choline acetyltransferase. Biochem. J. 100:1966;479-484.
6. Hagiwara M., Alberts A., Brindle P., Meinkoth J., Feramisco J., Deng T., Karin M., Shenolikar S., Montminy M. Transcriptional attenuation following cAMP induction requires PP-1-mediated dephosphorylation of CREB. Cell. 70:1992;105-113.
7. Inoue H., Li Y.P., Wagner J.A., Hersh L.B. Expression of the choline acetyltransferase gene depends on protein kinase A activity. J. Neurochem. 64:1995;985-990.
8. Johnson M.K. The intracellular distribution of glycolytic and other enzymes in rat brain homogenates and mitochondrial preparations. Biochem. J. 77:1960;610-618.
9. 2+-mediated endonuclease activation. Science. 242:1988;256-259.
10. Misawa H., Takahashi R., Deguchi T. Transcriptional regulation of choline acetyltransferase gene by cyclic AMP. J. Neurochem. 60:1993;1383-1387.
11. Otten L.A., Steimle V., Bontron S., Mach B. Quantitative control of MHC class II expression by the transactivator CIITA. Eur. J. Immunol. 28:1998;473-478.
12. Quirin-Stricker C., Nappey V., Simoni P., Toussaint J.L., Schmidt M. Trans-activation by thyroid hormone receptors of the 5' flanking region of the human ChAT gene. Molec. Brain Res. 23:1994;253-265.
13. Rieckmann P.C., Thevenin M., Kehrl J.H. Okadaic acid is a potent inducer of AP-1, NF-KB, and tumor necrosis factor-α in human B lymphocytes. Biochem. Biophys. Res. Commun. 187:1992;51-57.
14. Rossier J., Bauman A., Benda P. Improved purification of rat brain choline acetyltransferase by using an immunoabsorbent. FEBS Lett. 32:1973;231-234.
15. Salvaterra P.M., McCaman R.E. Choline acetyltransferase and acetylcholine levels in Drosophila melanogaster: a study using two temperature-sensitive mutants. J. Neurosci. 5:1985;903-910.
16. Schmidt B.M., Rylett J.R. Phosphorylation of rat brain choline acetyltransferase and its relationship to enzyme activity. J. Neurochem. 61:1993;1774-1781.
17. Sheng M., Thompson M.A., Greenberg M.E. CREB: a Ca(2+)-regulated transcription factor phosphorylated by calmodulin-dependent kinases. Science. 252:1991;1427-1430.
18. Tucek S. Regulation of acetylcholine synthesis in the brain. J. Neurochem. 44:1985;11-22.
19. Wadzinski B.E., Wheat W.H., Jaspers S., Peruski L.F., Lickteig R.L., Johnson G.L., Klemm D.J. Nuclear protein phosphatase 2A dephosphorylates protein kinase A-phosphorylated CREB and regulates CREB transcriptional stimulation. Mol. Cell Biol. 13:1993;2822-2834.
20. Wu D., Hersh L.B. Choline acetyltransferase: celebrating its fiftieth year. J. Neurochem. 62:1994;1653-1663.