The effect of agonists and antagonists on the morphology of non-transformed human smooth muscle cell in vitro

Journal of Submicroscopic Cytology and Pathology

Volumn 33, Issue 1-2, 2001, Pages 141-149

  Aliev, G ^a,b   Seyidova, D ^a,b   Neal, M L ^b   Shi, J ^b   Vigano, T ^c   Hernandez, A ^c   Folco, G ^c   Soas, A H ^b   Zimina, T V ^a   Smith, M A ^b   Perry, G ^b   Lamanna, J C ^a,b   Friedland, Robert P ^b  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF MILAN   (Italy)

Author keywords

Acetylcholine; Bradykinin; Electron microscopy; Human bronchi; Smooth muscle cells; Sulfidopeptide leukotriens

Indexed keywords

ACETYLCHOLINE; ACTIN; BRADYKININ; CALCIUM; DRUG DERIVATIVE; FURA 2; ICATIBANT; LEUKOTRIENE; MUSCARINIC AGENT; MUSCARINIC RECEPTOR BLOCKING AGENT; PROPIONIC ACID DERIVATIVE; QUINOLINE DERIVATIVE; VERLUKAST;

ARTICLE; BRONCHUS; CELL CULTURE; DOSE RESPONSE; DRUG EFFECT; HUMAN; ISOELECTRIC POINT; METABOLISM; MICROVILLUS; PATHOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SCANNING ELECTRON MICROSCOPY; SMOOTH MUSCLE; ULTRASTRUCTURE;

ACETYLCHOLINE; ACTINS; BRADYKININ; BRONCHI; CALCIUM; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FURA-2; HUMANS; ISOELECTRIC POINT; LEUKOTRIENES; MICROSCOPY, ELECTRON, SCANNING; MICROVILLI; MUSCARINIC AGONISTS; MUSCARINIC ANTAGONISTS; MUSCLE, SMOOTH; PROPIONATES; QUINOLINES;

EID: 0035236951     PISSN: 11229497     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. ALIEV G., CIRILLO R., SALVATICO E., PARO M. and PROSDOCIMI M., 1993. Changes in vessel ultrastructure during ischemia and reperfusion of rabbit hindlimb: implications for therapeutic intervention. Microvasc. Res., 46, 65-76.
2. ALIEV G., MIAH S., TURMAINE M. and BURNSTOCK G., 1995. An ultrastructural and immunocytochemical study of thoracic aortic endothelium in aged Sprague-Dawley rats. J. Submicrosc. Cytol. Pathol., 27, 477-490.
3. ALIEV G., RALEVIC V. and BURNSTOCK G., 1996. Depression of endothelial nitric oxide synthase but increased expression of endothelin-1 immunoreactivity in rat thoracic aortic endothelium associated with long-term, but not short-term, sympathectomy. Circ. Res., 79, 317-323.
4. ALIEV G., BODIN P. and BURNSTOCK G., 1998. Free radical generators cause changes in endothelial and inducible nitric oxide synthases and endothelin-1 immunoreactivity in endothelial cells from hyperlipidemic rabbits. Mol. Genet. Metab., 63, 191-197.
5. BATHON J.M., CROGHAN J.C., MACGLASHAN D.W. JR. and PROUD D., 1994. Bradykinin is a potent and relatively selective stimulus for cytosolic calcium elevation in human synovial cells. J. Immunol., 153, 2600-2608.
6. FAY F.S. and DELISE C.M., 1973. Contraction of isolated smooth-muscle cells-structural changes. Proc. Natl. Acad. Sci. U.S.A., 70, 641-645.
7. GABBIANI G., SCHMID E., WINTER S., CHAPONNIER C., DE CKHASTONAY C., VANDEKERCKHOVE J., WEBER K. and FRANKE W.W., 1981. Vascular smooth muscle cells differ from other smooth muscle cells: predominance of vimentin filaments and a specific alpha-type actin. Proc. Natl. Acad. Sci. U.S.A., 78, 298-302.
8. GARRELS J.I. and GIBSON W., 1976. Identification and characterization of multiple forms of actin. Cell, 9, 793-805.
9. GEPPETTI P., 1993. Sensory neuropeptide release by bradykinin: mechanisms and pathophysiological implications. Regul. Pept., 47, 1-23.
10. GIANAZZA E C.F.M.S.E.C.R.P., 2000a. Formulations for immobilized pH gradients including pH extremes. Electrophoresis, 10, 806-808.
11. GIANAZZA E T.G.C.N.B.L.R.P., 2000b. Properties of thin-rod immobilized pH gradients. Electrophoresis, 9, 172-182.
12. GLUKHOVA M.A., KABAKOV A.E., FRID M.G., ORNATSKY O.I., BELKIN A.M., MUKHIN D.N., OREKHOV A.N., KOTELIANSKY V.E. and SMIRNOV V.N., 1988. Modulation of human aorta smooth muscle cell phenotype: a study of muscle-specific variants of vinculin, caldesmon, and actin expression. Proc. Natl. Acad. Sci. U.S.A., 85, 9542-9546.
13. GRYNKIEWICZ G., POENIE M. and TSIEN R.Y., 1985. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J. Biol. Chem., 260, 3440-3450.
14. HALL I.P., DAYKIN K. and WIDDOP S., 1993. Beta 2-adrenoceptor desensitization in cultured human airway smooth muscle. Clin. Sci. (Colch.), 84, 151-157.
15. IVES H.E., SCHULTZ G.S., GALARDY R.E. and JAMIESON J.D., 1978. Preparation of functional smooth muscle cells from the rabbit aorta. J. Exp. Med., 148, 1400-1413.
16. JONES T.R. and MASSON P., 1985. Comparative study of the pulmonary effects of intravenous leukotrienes and other bronchoconstrictors in anaesthetized guinea pigs. Prostaglandins, 29, 799-817.
17. KAWAMOTO S. and ADELSTEIN R.S., 1987. Characterization of myosin heavy chains in cultured aorta smooth muscle cells. A comparative study. J. Biol. Chem., 262, 7282-7288.
18. LAEMMLI U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
19. LARSON D.M., FUJIWARA K., ALEXANDER R.W. and GIMBRONE M.A. JR., 1984. Myosin in cultured vascular smooth muscle cells: immunofluorescence and immunochemical studies of alterations in antigenic expression. J. Cell Biol., 99, 1582-1589.
20. MARSH K.A. and HILL S.J., 1993. Characteristics of the bradykinin-induced changes in intracellular calcium ion concentration of single bovine tracheal smooth muscle cells. Br. J. Pharmacol., 110, 29-35.
21. MARTHAN R., MARTIN C., AMEDEE T. and MIRONNEAU J., 1989a. Calcium channel currents in isolated smooth muscle cells from human bronchus. J. Appl. Physiol., 66, 1706-1714.
22. MATTOLI S., MEZZETTI M., RIVA G., ALLEGRA L. and FASOLI A., 1990. Specific binding of endothelin on human bronchial smooth muscle cells in culture and secretion of endothelin-like material from bronchial epithelial cells. Am. J. Respir. Cell Mol. Biol., 3, 145-151.
23. MOLIMARD M., MARTIN C.A., NALINE E., HIRSCH A. and ADVENIER C., 1994. Contractile effects of bradykinin on the isolated human small bronchus. Am. J. Respir. Crit. Care Med., 149, 123-127.
24. PANETTIERI R.A., MURRAY R.K., DEPALO L.R., YADVISH P.A. and KOTLIKOFF M.I., 1989b. A human airway smooth muscle cell line that retains physiological responsiveness. Am. J. Physiol., 256, C329-C335.
25. RICCIARDOLO FL. T.M.G.P.N.J.A.S.B.C., 2000. Role of nitric oxide and septide-insensitive NK(1) receptors in bronchoconstriction induced by aerosolised neurokinin A in guinea pigs. Br. J. Pharmacol., 129, 915-920.
26. SHIRINSKY V.P., BIRUKOV K.G., SOBOLEVSKY A.V., VEDERNIKOV Y.P., POSIN E.Y. and POPOV E.G., 1992. Contractile rabbit aortic smooth muscle cells in culture. Preparation and characterization. Am. J. Hypertens., 5, 124S-130S.
27. STEWART A.G., TOMLINSON P.R. and WILSON J., 1993. Airway wall remodelling in asthma: a novel target for the development of anti-asthma drugs. Trends Pharmacol. Sci., 14, 275-279.
28. TAKETA K I.E.H.T., 2000. A tetrazolium method for staining peroxidase labels in blotting assays. J. Immunol Methods, 95, 71-77.
29. TOKUNO H. and TOMITA T., 1987. Collagenase eliminates the electrical responses of smooth muscle to catecholamines. Eur. J. Pharmacol., 141, 131-133.
30. TOWBIN H., STAEHELIN T. and GORDON J., 1992. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. 1979 [classical article]. Biotechnology, 24, 145-149.
31. TSIEN R.Y., POZZAN T. and RINK T.J., 1982. Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator. J. Cell Biol., 94, 325-334.
32. VERZOLA B C.F.M.G.R.P., 2000. Monitoring equilibria and kinetics of protein folding/unfolding reactions by capillary zone electrophoresis. Anal. Biochem., 282, 239-244.
33. VIGANO T., HERNANDEZ A., CORSINI A., GRANATA A., BELLONI P., FUMAGALLI R., PAOLETTI R. and FOLCO G., 1995. Mevalonate pathway and isoprenoids regulate human bronchial myocytes proliferation. Eur. J. Pharmacol., 291, 201-203.
34. XUAN W P.J.T.B.M.G.B.G.W.A., 2000. Lung function growth and its relation to airway hyperresponsiveness and recent wheeze. Results from a longitudinal population study. Am. J. Respir. Crit. Care Med., 161, 1820-1824.