Genotoxicity of a crude leaf extract of neem in male germ cells of mice

Cytobios

Volumn 413, Issue 2 SUPPL., 2001, Pages 151-164

  Awasthy, K S ^a  

^a KKM College   (India)

Author keywords

Azadirachtin; Clastogenicity; Genotoxicity; Neem

Indexed keywords

ACYLGLYCEROL; CHEMOSTERILANT; CONTRACEPTIVE AGENT; NEEM OIL; PLANT EXTRACT; TERPENE;

ANIMAL; ARTICLE; CELL SIZE; CHEMISTRY; CHROMOSOME BREAKAGE; DOSE RESPONSE; DRUG EFFECT; GERM CELL; MALE; MEIOSIS; MOUSE; MUTAGEN TESTING; PATHOLOGY; PLANT LEAF; SPERMATOZOON COUNT;

ANIMALS; CELL SIZE; CHEMOSTERILANTS; CHROMOSOME BREAKAGE; CONTRACEPTIVE AGENTS, FEMALE; DOSE-RESPONSE RELATIONSHIP, DRUG; GERM CELLS; GLYCERIDES; MALE; MEIOSIS; MICE; MUTAGENICITY TESTS; PLANT EXTRACTS; PLANT LEAVES; SPERM COUNT; TERPENES;

EID: 0035231554     PISSN: 00114529     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. ADLER I. D. 1982. Mouse spermatogonia and spermatocyte sensitivity to chemical mutagens. Cytogenet. Cell Genet. 33 95-100.
2. ASHBY J. and Morrod R. S. 1991. Detection of human carcinogens. Nature 352 185-6.
3. AWASTHY K. S., Chaurasia O. P. and Sinha S. P. 1995. Genotoxic effects of crude extract of neem (Azadirachta indica) in bone marrow cells of mice. Cytologia 60 189-95.
4. AWASTHY K. S., Chaurasia O. P. and Sinha S. P. 1999. Prolonged murine genotoxic effects of crude extracts from neem. Phytother. Res. 12 81-3.
5. BATEMAN A. J. 1984. The dominant lethal assay in the male mouse. In Handbook of Mutagenic Test Procedures. Second editn. pp 471-84. Edited by B. J. Kilbey, M. Legator, M. Nichols and C. Ramel. Elsevier, Amsterdam.
6. BHUNYA S. P. and Behera B. C. 1987. Relative genotoxicity of trichlore acetic acid as revealed by different cytogenetic assays: bone marrow chromosome aberration, micronucleus and sperm head abnormality in the mouse. Mutat. Res. 188 215-21.
7. BUSHBY W. F. Jr and Wogan G. N. 1984. Aflatoxins. In Chemical Carcinogens. Vol 2, pp 945-1135. Edited by C. F. Searle. American Chemical Society, Washington, D.C.
8. CHATTERJEE S. 1995. How does neem oil make the sperm incompetant: a clue. Int. Sym. Pros of zona pellucide glycoprotein for Immunocontraception; 7th Ann. Conference. Indian Soc. Study of Reprod. Fertil. (N II, New Delhi). 123 pp.
9. CHULASIRI M., Bunyapraphatsara N. and Moongkamdi P. 1992. Mutagenicity and antimutagenicity of hispidulin and hortensin the flavonoids from Millingtonia hortensis. Environ. molec. Mutagen. 20 307-12.
10. COMANDEUR J. N. M. and Vermeulen N. P. E. 1996. Cytotoxicity and cytoprotective activities of natural compounds. The case of curcumin. Xenobiotica 26 667-80.
11. DAS R. K. and Nayak R. 1988. Spermatocyte chromosome alterations in mice inoculated with antirabies vaccine and tetanus toxoid separately and in combination. Mutat. Res. 204 277-82.
12. DHALIWAL G. S., Singh J. and Dilawari V. K. 1993. Potential of neem in insect-pest management in rice. Proc. Fourth World Neem Conference, Bangalore, India. Neem Newsletter (Indian Agric. Res. Inst., New Delhi) 10, 16 (Abstract).
13. DOWNEI N. M. and Heath R. E. 1970. Basic statistical methods. Harper and Row, New York.
14. EHLING U. H. 1989. Germ cell mutations in mice standards for protecting the human genome. Mutat. Res. 212 43-53.
15. EVENSON D. P., Bear R. K., Jost L. K. and Gesch R. W. 1986. Toxicity of thiotepa on mouse spermatogenesis as determined by dual-parameter flow cytometry. Toxicol. appl. Pharmac. 82 151-63.
16. GARG S., Taluja V., Upadhyay S. N. and Talwar G. P. 1993. Studies on the contraceptive efficacy of praneem polyherbal cream. Contraception 4 591-6.
17. HIGASHIMOTO M., Purintrapiban J., Kataoka K., Kinouchi T., Vinit Ketkumnuem U., Akimoto S., Matsumoto H. and Ohnishi Y. 1993. Mutagenicity and antimutagenicity of extracts of three spices and medicinal plant in Thailand. Mutat. Res. 303 135-42.
18. HOLDEN H. E. 1982. Comparison of somatic and germ cell models for cytogenetic screening. J. appl. Toxicol. 2 196-200.
19. HUGENHOLTZ A. P. and Bruce W. R. 1979. Sperm size abnormalities in homozygous and heterozygous in 59 RK mice. Can. J. Genet. Cytol. 21 115-9.
20. KHAN P. K. and Sinha S. P. 1994. Vitamin C mediated amelioration of pesticide genotoxicity in murine spermatocytes. Cytobios 80 199-204.
21. KHAN P. K. and Sinha S. P. 1996. Ameliorating effect of vitamin C on murine sperm toxicity induced by three pesticides (endosulfan, phosphamidon and mancozab). Mutagenesis 11 33-6.
22. KIRKLAND D. J. 1993. Genetic toxicology testing requirements: official and unofficial views from Europe. Molec. Toxicol. 21 8-14.
23. KOUL O., Isman M. B. and Ketkar C. M. 1990. Properties and uses of neem, Azadirachta indica. Can. J. Bot. 68 1-11.
24. KRZANOWSKA H. 1976a. Types of sperm-head abnormalities in four inbred strains of mice. Acta Biol. Sercra Zoologie 19 79-85.
25. KRZANOWSKA H. 1976b. Inheritance of sperm head abnormality types in mice and the role of the Y chromosome. Genet. Res. 28 189-98.
26. KUSAMRAN W. R., Ratnavila A. and Tapesuwan A. 1998. Effect of neem flower. Thai and Chinese bitter ground fruits and sweet basil leaves on hepatic monooxy genases and glutathione s-transferase activity, and in vitro metabolic activation of chemical carcinogens in rats. Food Chem. Toxicol. 36 475-84.
27. MANNA G. K. 1985. The living mutagens. In Laboratory Manual of Instructional Workshop on Perspectives in Chromosome Research. pp 55-72. Edited by S. C. Roy. Calcutta University Press, Calcutta.
28. MEISTRICH M. L. 1989. Evaluation of reproductive toxicity by testicular sperm head counts. J. Am. Coll. Toxicol. 8 55-567.
29. NATIONAL Research Council 1992. Neem: a tree for solving global problems. National Academy Press, Washington, D.C.
30. OAKBERG E. E. 1957. Duration of spermatogenesis in mouse. Nature 180 1137-9.
31. PADMA P. R., Amonkar A. J. and Bhide S. V. 1989. Antimutagenic effects of betel leaf extract against the mutagenicity of two tabacco specific N-nitrosamines. Mutagenesis 4 154-6.
32. RASTOGI P. B. and Levin R. E. 1987. Induction of sperm abnormalities in mice by quercetin. Environmental Mutagenesis 9 255-60.
33. ROSENKRANZ H. S. and Klopman G. 1995a. An examination of the potential genotoxic carcinogenicity of a biopesticide derived from the neem tree. Mutagenesis 26 255-60.
34. ROSENKRANZ H. S. and Klopman G. 1995b. Relationship between electronegativity and genotoxicity. Mutat. Res. 328 215-27.
35. SALVADORI D. M. F., Rebeiro L. R., Pereiro C. A. B. and Bacak W. 1988. Cytogenetic effects of malathion insecticide on somatic and germ cells of mice. Mutat. Res. 204 283-7.
36. SARKAR Datta D., Sharma A. and Talukdar G. 1996. Clastogenic activity of pure chlorophyll and anticlastogenic effects of equivalent amount of crude extract of Indian spinach leaf and chlorophyllin following dietary supplementation to mice. Environ. molec. Mutagen. 28 121-6.
37. SHARMA S., Stutzman J. D., Kelloff G. J. and Steel V. E. 1994. Screening of potential chemopreventive agents using biochemical markers of carcinogenesis. Cancer Res. 54 5848-55.
38. SIES H. 1993. Strategies of antioxidant defense. Eur. J. Biochem. 215 213-9.
39. SINGH R. N., Karnan P., Kulshrestha V. and Sinha S. S. 1993. Neem to control pests of tasar. Indian Silk 32 42-4.
40. SINHA S. P. and Prasad V. 1990. Effect of dietary concentration of crude aflatoxin on meiotic chromosomes, sperm morphology and sperm count in mice, Mus musculus. Proc. Indian natn. Sci. Acad. B56 269-76.
41. 1-containing Aspergillus flavus infested food. Cytobios 79 85-95.
42. STONE R. 1992. A biopesticidal tree begins to blossom. Science 255 1070-1.
43. TOPHAM J. C. 1980. Do induced sperm head abnormalities in mice specifically identify mammalian mutagens rather than carcinogens? Mutat. Res. 74 379-87.
44. UKEMS 1990. Basic mutagenic test. In UKEMS Recommended Procedures. Edited by D. S. Kirkland. Cambridge University Press, Cambridge, England.
45. UPADHYAY S. N. and Talwar G. P. 1993. Antifertility effect of neem, A. indica, oil in male rats by single intra-vas administration. An alternate approach to vasectomy. J. Androl. 14 275-81.
46. VAN DER NAT M. G., Van Der Sluis K., Desilva T. D. and Labdie R. P. 1991. Ethnopharmocognostical survey of A. indica Juss (Meliaceae). J. Ethnopharm. 35 1-24.
47. WYROBEK A. J. and Bruce W. R. 1978. The induction of sperm shape abnormalities in mice and humans. In Chemical Mutagens: Principles and Methods for their Detection. Vol 5, pp 257-85. Edited by A. Hollaender. Plenum Press, New York.