Genetic design of stable metal-binding biomolecules, oligomeric metallothioneins

Biocatalysis and Biotransformation

Volumn 19, Issue 5-6, 2001, Pages 399-412

  Murooka, Y ^a   Toyama, M ^a   Hong, S H ^a   Gohya, M ^a   Ono, H ^a   Yamashita, M ^a   Hirayama, N ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Cd; Human metallothionein II; Metal tolerance; Overproduction; Stabilization; Zn

Indexed keywords

BIOREMEDIATION; DETOXIFICATION; DIMERS; GENES; HEAVY METALS; MONOMERS; PROTEINS;

BIOMOLECULES;

ESCHERICHIA COLI;

CADMIUM; CYSTEINE; DITHIOTHREITOL; GLYCINE; HEAVY METAL; METALLOTHIONEIN; OLIGOMER; ZINC;

BACTERIAL GENETICS; BINDING AFFINITY; CELL MEMBRANE RESISTANCE; CELL NUCLEUS INCLUSION BODY; CODON; CONFERENCE PAPER; DETOXIFICATION; DIMERIZATION; ENZYME STABILITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; HUMAN; METAL BINDING; MOLECULAR GENETICS; MOLECULAR INTERACTION; MOLECULAR SIZE; NONHUMAN; OXIDATION; PROKARYOSIS; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA;

EID: 0035213799     PISSN: 10242422     EISSN: None     Source Type: Journal    
DOI: 10.3109/10242420108992026     Document Type: Conference Paper

References (32)

1. Amplification of the metallothionein-I gene in cadmium-resistant mouse cells
2. Efficient expression of the yeast metallothionein gene in Escherichia coli
3. The physiological function of metallothionein
4. Metallothionein protects DNA from oxidative damage
5. Degradation of hepatic zinc-thionein afterparental zinc administration
6. High yield expression and single step purification of human thioneim/metallothioneim
7. Expression of the mouse metallothionein-I gene in Escherichia coli: Increased tolerance to heavy metals
8. Control of zinc transfer between thionein, metallothionein, and zinc proteins
9. Biochemistry of metallothionein
10. Equine hepatic and neural metallothioneins. Purification, molecular weight, amino acid composition, and metal content
11. The expression of a synthetic rainbow trout metallothionein gene
12. Kinetic liability of zinc bound to metallothionein in Ehrlich cells
13. Solid-phase peptide synthesis of the α and β domains of liver metallothionein 2 and the metallothionein of Neurospora crassa
14. Construction of an artificial bifunctional enzyme, beta-galactosidase/galactose dehydrogenase, exhibiting efficient galactose channeling
15. A cadmium protein from equine kidney cortex
16. L promoters in Escherichia coli
17. L functional in enteric bacteria
18. Expression of cloned monkey metallothionein in Escherichia coli
19. Developmental regulation, induction, and embrionic tissue specificity of sea urchin metallothionein gene expression
20. Expression of human metallothionein-2 in Escherichia coli: Cadmium tolerance of transformed cells
21. Amino acids and peptides. VIII. Synthesis of a hexacosapeptide corresponding to the C-terminal sequence 36-61 of human metallothionein II (MTII) and determination of its heavy metal binding activity
22. Synthesis of nanocosapeptide (β-fragment) corresponding to the N-terminal sequence 1-29 of human liver metallothionein II and its heavy metal-binding properties
23. Interprotein metal exchange reactions of metallothionein
24. Reactivity of the thiol group in human and bovine albumin at pH 3-9, as measured by exchange with 2,2′-dithiodipyridine
25. Differential sensitivity of metallothionein-I and -II in liver of zinc-injected rat toward proteolysis
26. Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli
27. Zinc (II), cadmium (II), and mercury (II) thiolate transition in metallothionein
28. Structural and functional studies of the amino terminus of yeast metallothionein
29. Purification and characteristics of recombinant mouse metallothionein-I from Escherichia coli
30. Genetic design of a gene for human metallothionein II and its expression as an active fusion protein in Escherichia coli
31. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of M13mp18 and pUC19 vectors