Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize

Theoretical and Applied Genetics

Volumn 103, Issue 6-7, 2001, Pages 817-826

  Bohorova, N ^a   Frutos, R ^b   Royer, M ^b   Estanol P ^a   Pacheco, M ^a   Rascon Q ^a   McLean, S ^a   Hoisington, D ^a  

^a INTERNATIONAL MAIZE AND WHEAT IMPROVEMENT CENTER CIMMYT   (Mexico)

^b CIRAD   (France)

Author keywords

Bacillus thuringiensis (Bt); Insect resistance; Plant regeneration; Transformation; Tropical maize

Indexed keywords

BACTERIAL GENE; GENE EXPRESSION; GENETIC TRANSFORMATION; HYBRID PROTEIN; INHERITANCE; INSECT RESISTANCE; MAIZE; MICROPROJECTILE BOMBARDMENT; MOLECULAR ANALYSIS; PROGENY; PROMOTER REGION; PROTEIN EXPRESSION; RESISTANCE MANAGEMENT; TRANSGENIC PLANT; UBIQUITIN;

CLONING; PEST CONTROL; PLANTS (BOTANY); PROTEINS;

BACILLUS THURINGIENSIS;

GENES;

BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); DIATRAEA SACCHARALIS; EMBRYOPHYTA; HEXAPODA; INSECTA; MYTHIMNA UNIPUNCTA; SACCHARUM HYBRID CULTIVAR; SPODOPTERA FRUGIPERDA; ZEA MAYS;

EID: 0035207058     PISSN: 00405752     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001220100686     Document Type: Article

References (41)

1. The reconstruction and expression of a Bacillus thuringiensis cryIIIA gene in protoplasts and potato plants
2. Field evaluation of european corn borer control in progeny of 173 transgenic corn events expressing an insecticidal protein from Bacillus thuringiensis
3. Efficacy and deployment of transgenic plants for stemborer management
4. Regeneration potential of tropical, subtropical, midaltitude, and highland maize inbreds
5. Selection and characterization of Mexican strains of Bacillus thuringiensis Berl. active against four major lepidopteran maize pests
6. Susceptibility of four tropical lepidopteran maize pest to Bacillus thuringiensis cryI type insecticidal toxins
7. Production of transgenic tropical maize with cry1Ab and cryIAc genes via microprojectile bombardment of immature embryos
8. Laboratory protocols
9. Maize polyubiquitin genes: Structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation
10. PCR analysis of the cryI insecticidal crystal family genes from Bacillus thuringiensis
11. mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo
12. Transgenic plants: An emerging approach to pest control
13. Quantative analysis of cryIA(b) expression in Bt maize plants, tissue and silage, and stability of expression over successive generations
14. Inheritance and expression of chimeric genes in the progeny of transgenic maize plants
15. Managing insect resistance to plants producing Bacillus thuringiensis toxins
16. Insect-resistant rice generated by the introduction of a modified delta-endotoxin gene of Bacillus thuringiensis
17. Transformation of maize cells and regeneration of fertile transgenic plants
18. A translation fusion product of two different insecticidal crystal protein genes of Bacillus thuringiensis exhibits an enlarged insecticidal spectrum
19. Genetic transformation of maize cells by particle bombardment
20. Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis
21. What determines the instability of c-myc proto-oncogene mRNA
22. Bt resistance management
23. Reduced fusarium ear rot and symptomless infection in kernels of maize genetically engineered for European corn borer resistance
24. Codon usage in plant genes
25. Analysis of unstable RNA transcripts of insecticidal crystal protein genes of Bacillus thuringiensis in transgenic plants and electroporated protoplasts
26. Progress and prospects for field use of Bt genes in crops
27. Insect resistant cotton plants
28. Modification of the coding sequence enhances plant expression of insect control protein genes
29. Genetically improved potatoes: Protection from damage by colorado potato beetles
30. Recursive PCR: A novel technique for total gene synthesis
31. Simultaneous production of the 34-kDa and 40-kDa proteins from Bacillus thuringiensis subsp. Thompsoni is required for the formation of inclusion bodies
32. Construction and rapid testing of synthetic and modified toxin gene sequences cryIA (b and c) by expression in maize endosperm culture
33. A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation
34. Molecular identification and isolation of the Waxy locus in maize
35. Bialaphos selection of stable transformants from maize cell culture
36. A synthetic cry1C gene, encoding a Bacillus thuringiensis-endotoxin, confer Spodoptera resistance in alfalfa and tobacco
37. Managing resistance to Bacillus thuringiensis: Lessons from the diamondback moth (Lepidoptera: Plutellidae)
38. Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: A resistance management strategy
39. Transgenic corn evaluated for resistance to fall army-worm and southweatern corn borer
40. Cloning and analysis of the first cry gene from B. popiliae